Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain
Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associ...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Centers for Disease Control and Prevention
2017-12-01
|
Series: | Emerging Infectious Diseases |
Subjects: | |
Online Access: | https://wwwnc.cdc.gov/eid/article/23/12/15-1146_article |
id |
doaj-0852d8c062c6445eaabfbca52457d33a |
---|---|
record_format |
Article |
spelling |
doaj-0852d8c062c6445eaabfbca52457d33a2020-11-25T01:55:11ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592017-12-0123122013201610.3201/eid2312.151146Characterization of Streptococcus pyogenes from Animal Clinical Specimens, SpainAna Isabel VelaPilar VillalónJuan Antonio Sáez-NietoGema ChacónLucas DomínguezJosé Francisco Fernández-GarayzábalStreptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited the M macrolide resistance phenotype and the mefA/ermB genotype. Isolates were grouped into 2 clones on the basis of emm-MLST-PFGE-virulence gene profile combinations: clone 1, characterized by the combined genotype emm12-ST36-pulsotype A-speG; and clone 2, characterized by the genotype emm77-ST63-pulsotype B-speC. Our results do not show conclusively that animals may represent a new reservoir of S. pyogenes but indicate the ability of human-derived S. pyogenes isolates to colonize and infect animals.https://wwwnc.cdc.gov/eid/article/23/12/15-1146_articleStreptococcus pyogenesanimalscharacterizationdetectionbacteriaSpain |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana Isabel Vela Pilar Villalón Juan Antonio Sáez-Nieto Gema Chacón Lucas Domínguez José Francisco Fernández-Garayzábal |
spellingShingle |
Ana Isabel Vela Pilar Villalón Juan Antonio Sáez-Nieto Gema Chacón Lucas Domínguez José Francisco Fernández-Garayzábal Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain Emerging Infectious Diseases Streptococcus pyogenes animals characterization detection bacteria Spain |
author_facet |
Ana Isabel Vela Pilar Villalón Juan Antonio Sáez-Nieto Gema Chacón Lucas Domínguez José Francisco Fernández-Garayzábal |
author_sort |
Ana Isabel Vela |
title |
Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain |
title_short |
Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain |
title_full |
Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain |
title_fullStr |
Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain |
title_full_unstemmed |
Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain |
title_sort |
characterization of streptococcus pyogenes from animal clinical specimens, spain |
publisher |
Centers for Disease Control and Prevention |
series |
Emerging Infectious Diseases |
issn |
1080-6040 1080-6059 |
publishDate |
2017-12-01 |
description |
Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited the M macrolide resistance phenotype and the mefA/ermB genotype. Isolates were grouped into 2 clones on the basis of emm-MLST-PFGE-virulence gene profile combinations: clone 1, characterized by the combined genotype emm12-ST36-pulsotype A-speG; and clone 2, characterized by the genotype emm77-ST63-pulsotype B-speC. Our results do not show conclusively that animals may represent a new reservoir of S. pyogenes but indicate the ability of human-derived S. pyogenes isolates to colonize and infect animals. |
topic |
Streptococcus pyogenes animals characterization detection bacteria Spain |
url |
https://wwwnc.cdc.gov/eid/article/23/12/15-1146_article |
work_keys_str_mv |
AT anaisabelvela characterizationofstreptococcuspyogenesfromanimalclinicalspecimensspain AT pilarvillalon characterizationofstreptococcuspyogenesfromanimalclinicalspecimensspain AT juanantoniosaeznieto characterizationofstreptococcuspyogenesfromanimalclinicalspecimensspain AT gemachacon characterizationofstreptococcuspyogenesfromanimalclinicalspecimensspain AT lucasdominguez characterizationofstreptococcuspyogenesfromanimalclinicalspecimensspain AT josefranciscofernandezgarayzabal characterizationofstreptococcuspyogenesfromanimalclinicalspecimensspain |
_version_ |
1724984581705170944 |