Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro
Zuo Gui Wan (ZGW) and You Gui Wan (YGW) are two classic formulas used in clinical treatment of infertility in traditional Chinese medicine (TCM). However, the actions of the formulas remain to be proven at the cellular and molecular levels. In this study, we investigate whether the two formulas have...
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doaj-086e3efa3883483db261713c6e20cab12020-11-24T23:58:39ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882015-01-01201510.1155/2015/825805825805Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In VitroXiang Hu0Hua Lu1Yan-li Deng2Qian Wan3Shang-mian Yie4Department of Gynecology and Obstetrics, The Second Medical College/Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Remin Nanlu Road, Chengdu, Sichuan 610041, ChinaDepartment of Gynecology and Obstetrics, The Second Medical College/Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Remin Nanlu Road, Chengdu, Sichuan 610041, ChinaDepartment of Gynecology and Obstetrics, The Second Medical College/Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Remin Nanlu Road, Chengdu, Sichuan 610041, ChinaDepartment of Gynecology and Obstetrics, The Second Medical College/Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Remin Nanlu Road, Chengdu, Sichuan 610041, ChinaDepartment of Gynecology and Obstetrics, The Second Medical College/Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Remin Nanlu Road, Chengdu, Sichuan 610041, ChinaZuo Gui Wan (ZGW) and You Gui Wan (YGW) are two classic formulas used in clinical treatment of infertility in traditional Chinese medicine (TCM). However, the actions of the formulas remain to be proven at the cellular and molecular levels. In this study, we investigate whether the two formulas have any effect on germ cell formation and differentiation by culturing rat medicated serums containing YGW or ZGW with stem cells derived from human first trimester umbilical cord. Our results showed that while the normal rat serums had no significant effects, the rat medicated serums had significant effects on the differentiation of the stem cells into oocyte-like cells (OLCs) based on (1) cell morphological changes that resembled purative cumulus-oocyte complexes (COCs); (2) expressions of specific markers that were indicative of germ cell formation and oocyte development; and (3) estradiol production by the COC-like cells. Furthermore, ZGW medicated serums exhibited more obvious effects on specific gene expressions of germ cells, whereas YGW medicated serums showed stronger effects on estradiol production. Accordingly, our study provides evidence demonstrating for the first time that one of molecular and cellular actions of YGW or ZGW in treating human reproductive dysfunctions may be through an enhancement of neooogenesis.http://dx.doi.org/10.1155/2015/825805 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiang Hu Hua Lu Yan-li Deng Qian Wan Shang-mian Yie |
spellingShingle |
Xiang Hu Hua Lu Yan-li Deng Qian Wan Shang-mian Yie Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro Evidence-Based Complementary and Alternative Medicine |
author_facet |
Xiang Hu Hua Lu Yan-li Deng Qian Wan Shang-mian Yie |
author_sort |
Xiang Hu |
title |
Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro |
title_short |
Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro |
title_full |
Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro |
title_fullStr |
Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro |
title_full_unstemmed |
Effect of Rat Medicated Serum Containing Zuo Gui Wan and/or You Gui Wan on the Differentiation of Stem Cells Derived from Human First Trimester Umbilical Cord into Oocyte-Like Cells In Vitro |
title_sort |
effect of rat medicated serum containing zuo gui wan and/or you gui wan on the differentiation of stem cells derived from human first trimester umbilical cord into oocyte-like cells in vitro |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2015-01-01 |
description |
Zuo Gui Wan (ZGW) and You Gui Wan (YGW) are two classic formulas used in clinical treatment of infertility in traditional Chinese medicine (TCM). However, the actions of the formulas remain to be proven at the cellular and molecular levels. In this study, we investigate whether the two formulas have any effect on germ cell formation and differentiation by culturing rat medicated serums containing YGW or ZGW with stem cells derived from human first trimester umbilical cord. Our results showed that while the normal rat serums had no significant effects, the rat medicated serums had significant effects on the differentiation of the stem cells into oocyte-like cells (OLCs) based on (1) cell morphological changes that resembled purative cumulus-oocyte complexes (COCs); (2) expressions of specific markers that were indicative of germ cell formation and oocyte development; and (3) estradiol production by the COC-like cells. Furthermore, ZGW medicated serums exhibited more obvious effects on specific gene expressions of germ cells, whereas YGW medicated serums showed stronger effects on estradiol production. Accordingly, our study provides evidence demonstrating for the first time that one of molecular and cellular actions of YGW or ZGW in treating human reproductive dysfunctions may be through an enhancement of neooogenesis. |
url |
http://dx.doi.org/10.1155/2015/825805 |
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