Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily

The way we study cortical development has undergone a revolution in the last few years following the ability to use shRNA in the developing brain of the rodent embryo. The first gene to be knocked-down in the developing brain was doublecortin (Dcx). Here we will review knockdown experiments in the d...

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Bibliographic Details
Main Authors:	Raanan Greenman, Orly Reiner, Anna Gorelik
Format:	Article
Language:	English
Published:	MDPI AG 2012-11-01
Series:	Genes
Subjects:	doublecortin DCX shRNA in utero electroporation neuronal migration
Online Access:	http://www.mdpi.com/2073-4425/3/4/759

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spelling	doaj-087920985e664b58b41a378a1f74338d2020-11-24T23:10:40ZengMDPI AGGenes2073-44252012-11-013475977810.3390/genes3040759Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin SuperfamilyRaanan GreenmanOrly ReinerAnna GorelikThe way we study cortical development has undergone a revolution in the last few years following the ability to use shRNA in the developing brain of the rodent embryo. The first gene to be knocked-down in the developing brain was doublecortin (Dcx). Here we will review knockdown experiments in the developing brain and compare them with knockout experiments, thus highlighting the advantages and disadvantages using the different systems. Our review will focus on experiments relating to the doublecortin superfamily of proteins.http://www.mdpi.com/2073-4425/3/4/759doublecortinDCXshRNAin utero electroporationneuronal migration
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Raanan Greenman Orly Reiner Anna Gorelik
spellingShingle	Raanan Greenman Orly Reiner Anna Gorelik Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily Genes doublecortin DCX shRNA in utero electroporation neuronal migration
author_facet	Raanan Greenman Orly Reiner Anna Gorelik
author_sort	Raanan Greenman
title	Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_short	Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_full	Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_fullStr	Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_full_unstemmed	Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily
title_sort	use of rna interference by in utero electroporation to study cortical development: the example of the doublecortin superfamily
publisher	MDPI AG
series	Genes
issn	2073-4425
publishDate	2012-11-01
description	The way we study cortical development has undergone a revolution in the last few years following the ability to use shRNA in the developing brain of the rodent embryo. The first gene to be knocked-down in the developing brain was doublecortin (Dcx). Here we will review knockdown experiments in the developing brain and compare them with knockout experiments, thus highlighting the advantages and disadvantages using the different systems. Our review will focus on experiments relating to the doublecortin superfamily of proteins.
topic	doublecortin DCX shRNA in utero electroporation neuronal migration
url	http://www.mdpi.com/2073-4425/3/4/759
work_keys_str_mv	AT raanangreenman useofrnainterferencebyinuteroelectroporationtostudycorticaldevelopmenttheexampleofthedoublecortinsuperfamily AT orlyreiner useofrnainterferencebyinuteroelectroporationtostudycorticaldevelopmenttheexampleofthedoublecortinsuperfamily AT annagorelik useofrnainterferencebyinuteroelectroporationtostudycorticaldevelopmenttheexampleofthedoublecortinsuperfamily
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Use of RNA Interference by In Utero Electroporation to Study Cortical Development: The Example of the Doublecortin Superfamily

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