Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus
An enzymatic mixture of cellulases and xylanases was produced by Pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of Arundo donax bioconversion. The Plackett-Burman screening design was applied to identify the most signif...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2015-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2015/951871 |
id |
doaj-088b68a14dfb4b9ab3365df4b6a778c6 |
---|---|
record_format |
Article |
spelling |
doaj-088b68a14dfb4b9ab3365df4b6a778c62020-11-24T22:25:12ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/951871951871Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatusRossana Liguori0Elena Ionata1Loredana Marcolongo2Luciana Porto de Souza Vandenberghe3Francesco La Cara4Vincenza Faraco5Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, ItalyInstitute of Agro-Environment and Forest Biology, National Research Council (CNR), Via Pietro Castellino 111, 80131 Naples, ItalyInstitute of Agro-Environment and Forest Biology, National Research Council (CNR), Via Pietro Castellino 111, 80131 Naples, ItalyDepartment of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Coronel Francisco H. dos Santos Avenue 210, 81531-990 Curitiba, PR, BrazilInstitute of Agro-Environment and Forest Biology, National Research Council (CNR), Via Pietro Castellino 111, 80131 Naples, ItalyDepartment of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, ItalyAn enzymatic mixture of cellulases and xylanases was produced by Pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of Arundo donax bioconversion. The Plackett-Burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreated A. donax. As the most significant influence during the enzymatic hydrolysis of A. donax was exercised by the temperature (°C), pH, and time, the combined effect of these factors in the bioconversion by P. ostreatus cellulase and xylanase was analyzed by a 33 factorial experimental design. It is worth noting that the best result of 480.10 mg of sugars/gds, obtained at 45°C, pH 3.5, and 96 hours of incubation, was significant also when compared with the results previously reached by process optimization with commercial enzymes.http://dx.doi.org/10.1155/2015/951871 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rossana Liguori Elena Ionata Loredana Marcolongo Luciana Porto de Souza Vandenberghe Francesco La Cara Vincenza Faraco |
spellingShingle |
Rossana Liguori Elena Ionata Loredana Marcolongo Luciana Porto de Souza Vandenberghe Francesco La Cara Vincenza Faraco Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus BioMed Research International |
author_facet |
Rossana Liguori Elena Ionata Loredana Marcolongo Luciana Porto de Souza Vandenberghe Francesco La Cara Vincenza Faraco |
author_sort |
Rossana Liguori |
title |
Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus |
title_short |
Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus |
title_full |
Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus |
title_fullStr |
Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus |
title_full_unstemmed |
Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus |
title_sort |
optimization of arundo donax saccharification by (hemi)cellulolytic enzymes from pleurotus ostreatus |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
An enzymatic mixture of cellulases and xylanases was produced by Pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of Arundo donax bioconversion. The Plackett-Burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreated A. donax. As the most significant influence during the enzymatic hydrolysis of A. donax was exercised by the temperature (°C), pH, and time, the combined effect of these factors in the bioconversion by P. ostreatus cellulase and xylanase was analyzed by a 33 factorial experimental design. It is worth noting that the best result of 480.10 mg of sugars/gds, obtained at 45°C, pH 3.5, and 96 hours of incubation, was significant also when compared with the results previously reached by process optimization with commercial enzymes. |
url |
http://dx.doi.org/10.1155/2015/951871 |
work_keys_str_mv |
AT rossanaliguori optimizationofarundodonaxsaccharificationbyhemicellulolyticenzymesfrompleurotusostreatus AT elenaionata optimizationofarundodonaxsaccharificationbyhemicellulolyticenzymesfrompleurotusostreatus AT loredanamarcolongo optimizationofarundodonaxsaccharificationbyhemicellulolyticenzymesfrompleurotusostreatus AT lucianaportodesouzavandenberghe optimizationofarundodonaxsaccharificationbyhemicellulolyticenzymesfrompleurotusostreatus AT francescolacara optimizationofarundodonaxsaccharificationbyhemicellulolyticenzymesfrompleurotusostreatus AT vincenzafaraco optimizationofarundodonaxsaccharificationbyhemicellulolyticenzymesfrompleurotusostreatus |
_version_ |
1725758902497181696 |