Genetic diversity of Halla horses using microsatellite markers
Abstract Background Currently about 26,000 horses are breeding in Korea and 57.2% (14,776 horses) of them are breeding in Jeju island. According to the statistics published in 2010, the horses breeding in Jeju island are subdivided into Jeju horse (6.1%), Thoroughbred (18.8%) and Halla horse (75.1%)...
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Korean Society of Animal Sciences and Technology
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doaj-08a29427ee6b4ff0afd32826e86ef9742020-11-25T02:40:22ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2055-03912016-11-015811510.1186/s40781-016-0120-6Genetic diversity of Halla horses using microsatellite markersJoo-Hee Seo0Kyung-Do Park1Hak-Kyo Lee2Hong-Sik Kong3Genomic Informatics Center, Hankyong National UniversityChonBuk National UniversityChonBuk National UniversityGenomic Informatics Center, Hankyong National UniversityAbstract Background Currently about 26,000 horses are breeding in Korea and 57.2% (14,776 horses) of them are breeding in Jeju island. According to the statistics published in 2010, the horses breeding in Jeju island are subdivided into Jeju horse (6.1%), Thoroughbred (18.8%) and Halla horse (75.1%). Halla horses are defined as a crossbreed between Jeju and Thoroughbred horses and are used for horse racing, horse riding and horse meat production. However, little research has been conducted on Halla horses because of the perception of crossbreed and people’s weighted interest toward Jeju horses. Method Using 17 Microsatellite (MS) Markers recommended by International Society for Animal Genetics (ISAG), genomic DNAs were extracted from the hair roots of 3,880 Halla horses breeding in Korea and genetic diversity was identified by genotyping after PCR was performed. Results and conclusion In average, 10.41 alleles (from 6 alleles in HTG7 to 17 alleles in ASB17) were identified after the analysis using 17 MS Markers. The mean value of Hobs was 0.749 with a range from 0.612(HMS1) to 0.857(ASB2). Also, it was found that Hexp and PIC values were lowest in HMS1 (0.607 and 0.548, respectively), and highest in LEX3(0.859 and 0.843, respectively), and the mean value of Hexp was 0.760 and that of PIC was 0.728. 17 MS markers used in this studies were considered as appropriate markers for the polymorphism analysis of Halla horses. The frequency for the appearance of identical individuals was 5.90 × 10−20 when assumed as random mating population and when assumed as half-sib and full-sib population, frequencies were 4.08 × 10−15 and 3.56 × 10−8, respectively. Based on these results, the 17 MS markers can be used adequately for the Individual Identification and Parentage Verification of Halla horses. Remarkably, allele M and Q of ASB23 marker, G of HMS2 marker, H and L of HTG6 marker, L of HTG7 marker, E of LEX3 marker were the specific alleles unique to Halla horses.http://link.springer.com/article/10.1186/s40781-016-0120-6Microsatellite markerHalla HorsePolymorphismAllele frequency |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joo-Hee Seo Kyung-Do Park Hak-Kyo Lee Hong-Sik Kong |
spellingShingle |
Joo-Hee Seo Kyung-Do Park Hak-Kyo Lee Hong-Sik Kong Genetic diversity of Halla horses using microsatellite markers Journal of Animal Science and Technology Microsatellite marker Halla Horse Polymorphism Allele frequency |
author_facet |
Joo-Hee Seo Kyung-Do Park Hak-Kyo Lee Hong-Sik Kong |
author_sort |
Joo-Hee Seo |
title |
Genetic diversity of Halla horses using microsatellite markers |
title_short |
Genetic diversity of Halla horses using microsatellite markers |
title_full |
Genetic diversity of Halla horses using microsatellite markers |
title_fullStr |
Genetic diversity of Halla horses using microsatellite markers |
title_full_unstemmed |
Genetic diversity of Halla horses using microsatellite markers |
title_sort |
genetic diversity of halla horses using microsatellite markers |
publisher |
Korean Society of Animal Sciences and Technology |
series |
Journal of Animal Science and Technology |
issn |
2055-0391 |
publishDate |
2016-11-01 |
description |
Abstract Background Currently about 26,000 horses are breeding in Korea and 57.2% (14,776 horses) of them are breeding in Jeju island. According to the statistics published in 2010, the horses breeding in Jeju island are subdivided into Jeju horse (6.1%), Thoroughbred (18.8%) and Halla horse (75.1%). Halla horses are defined as a crossbreed between Jeju and Thoroughbred horses and are used for horse racing, horse riding and horse meat production. However, little research has been conducted on Halla horses because of the perception of crossbreed and people’s weighted interest toward Jeju horses. Method Using 17 Microsatellite (MS) Markers recommended by International Society for Animal Genetics (ISAG), genomic DNAs were extracted from the hair roots of 3,880 Halla horses breeding in Korea and genetic diversity was identified by genotyping after PCR was performed. Results and conclusion In average, 10.41 alleles (from 6 alleles in HTG7 to 17 alleles in ASB17) were identified after the analysis using 17 MS Markers. The mean value of Hobs was 0.749 with a range from 0.612(HMS1) to 0.857(ASB2). Also, it was found that Hexp and PIC values were lowest in HMS1 (0.607 and 0.548, respectively), and highest in LEX3(0.859 and 0.843, respectively), and the mean value of Hexp was 0.760 and that of PIC was 0.728. 17 MS markers used in this studies were considered as appropriate markers for the polymorphism analysis of Halla horses. The frequency for the appearance of identical individuals was 5.90 × 10−20 when assumed as random mating population and when assumed as half-sib and full-sib population, frequencies were 4.08 × 10−15 and 3.56 × 10−8, respectively. Based on these results, the 17 MS markers can be used adequately for the Individual Identification and Parentage Verification of Halla horses. Remarkably, allele M and Q of ASB23 marker, G of HMS2 marker, H and L of HTG6 marker, L of HTG7 marker, E of LEX3 marker were the specific alleles unique to Halla horses. |
topic |
Microsatellite marker Halla Horse Polymorphism Allele frequency |
url |
http://link.springer.com/article/10.1186/s40781-016-0120-6 |
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