Ftr82 Is Critical for Vascular Patterning during Zebrafish Development
Cellular components and signaling pathways are required for the proper growth of blood vessels. Here, we report for the first time that a teleost-specific gene ftr82 (finTRIM family, member 82) plays a critical role in vasculature during zebrafish development. To date, there has been no description...
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doaj-08b92479f17c4e368110b09f97db7a512020-11-24T23:53:11ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-01-0118115610.3390/ijms18010156ijms18010156Ftr82 Is Critical for Vascular Patterning during Zebrafish DevelopmentHsueh-Wei Chang0Wen-Der Wang1Chien-Chih Chiu2Chiou-Hua Chen3Yi-Shan Wang4Zih-Ying Chen5Wangta Liu6Ming-Hong Tai7Zhi-Hong Wen8Chang-Yi Wu9Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDepartment of Bioagricultural Science, National Chiayi University, Chiayi City 60004, TaiwanDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDepartment of Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, TaiwanInstitute of Biomedical Science, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDoctoral Degree Program in Marine Biotechnology, National Sun Yat-sen University and Academia Sinica, Kaohsiung 80424, TaiwanDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanCellular components and signaling pathways are required for the proper growth of blood vessels. Here, we report for the first time that a teleost-specific gene ftr82 (finTRIM family, member 82) plays a critical role in vasculature during zebrafish development. To date, there has been no description of tripartite motif proteins (TRIM) in vascular development, and the role of ftr82 is unknown. In this study, we found that ftr82 mRNA is expressed during the development of vessels, and loss of ftr82 by morpholino (MO) knockdown impairs the growth of intersegmental vessels (ISV) and caudal vein plexus (CVP), suggesting that ftr82 plays a critical role in promoting ISV and CVP growth. We showed the specificity of ftr82 MO by analyzing ftr82 expression products and expressing ftr82 mRNA to rescue ftr82 morphants. We further showed that the knockdown of ftr82 reduced ISV cell numbers, suggesting that the growth impairment of vessels is likely due to a decrease of cell proliferation and migration, but not cell death. In addition, loss of ftr82 affects the expression of vascular markers, which is consistent with the defect of vascular growth. Finally, we showed that ftr82 likely interacts with vascular endothelial growth factor (VEGF) and Notch signaling. Together, we identify teleost-specific ftr82 as a vascular gene that plays an important role for vascular development in zebrafish.http://www.mdpi.com/1422-0067/18/1/156ftr82TRIM familyangiogenesisISV (intersegmental vessel)CVP (caudal vein plexus)zebrafish |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hsueh-Wei Chang Wen-Der Wang Chien-Chih Chiu Chiou-Hua Chen Yi-Shan Wang Zih-Ying Chen Wangta Liu Ming-Hong Tai Zhi-Hong Wen Chang-Yi Wu |
spellingShingle |
Hsueh-Wei Chang Wen-Der Wang Chien-Chih Chiu Chiou-Hua Chen Yi-Shan Wang Zih-Ying Chen Wangta Liu Ming-Hong Tai Zhi-Hong Wen Chang-Yi Wu Ftr82 Is Critical for Vascular Patterning during Zebrafish Development International Journal of Molecular Sciences ftr82 TRIM family angiogenesis ISV (intersegmental vessel) CVP (caudal vein plexus) zebrafish |
author_facet |
Hsueh-Wei Chang Wen-Der Wang Chien-Chih Chiu Chiou-Hua Chen Yi-Shan Wang Zih-Ying Chen Wangta Liu Ming-Hong Tai Zhi-Hong Wen Chang-Yi Wu |
author_sort |
Hsueh-Wei Chang |
title |
Ftr82 Is Critical for Vascular Patterning during Zebrafish Development |
title_short |
Ftr82 Is Critical for Vascular Patterning during Zebrafish Development |
title_full |
Ftr82 Is Critical for Vascular Patterning during Zebrafish Development |
title_fullStr |
Ftr82 Is Critical for Vascular Patterning during Zebrafish Development |
title_full_unstemmed |
Ftr82 Is Critical for Vascular Patterning during Zebrafish Development |
title_sort |
ftr82 is critical for vascular patterning during zebrafish development |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-01-01 |
description |
Cellular components and signaling pathways are required for the proper growth of blood vessels. Here, we report for the first time that a teleost-specific gene ftr82 (finTRIM family, member 82) plays a critical role in vasculature during zebrafish development. To date, there has been no description of tripartite motif proteins (TRIM) in vascular development, and the role of ftr82 is unknown. In this study, we found that ftr82 mRNA is expressed during the development of vessels, and loss of ftr82 by morpholino (MO) knockdown impairs the growth of intersegmental vessels (ISV) and caudal vein plexus (CVP), suggesting that ftr82 plays a critical role in promoting ISV and CVP growth. We showed the specificity of ftr82 MO by analyzing ftr82 expression products and expressing ftr82 mRNA to rescue ftr82 morphants. We further showed that the knockdown of ftr82 reduced ISV cell numbers, suggesting that the growth impairment of vessels is likely due to a decrease of cell proliferation and migration, but not cell death. In addition, loss of ftr82 affects the expression of vascular markers, which is consistent with the defect of vascular growth. Finally, we showed that ftr82 likely interacts with vascular endothelial growth factor (VEGF) and Notch signaling. Together, we identify teleost-specific ftr82 as a vascular gene that plays an important role for vascular development in zebrafish. |
topic |
ftr82 TRIM family angiogenesis ISV (intersegmental vessel) CVP (caudal vein plexus) zebrafish |
url |
http://www.mdpi.com/1422-0067/18/1/156 |
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