Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China
Plasmodium falciparum surface-related antigen (SRA) is located on the surfaces of gametocyte and merozoite and has the structural and functional characteristics of potential targets for multistage vaccine development. However, little information is available regarding the genetic polymorphism of pfs...
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Frontiers Media S.A.
2021-08-01
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Series: | Frontiers in Genetics |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.688606/full |
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doaj-08bbd5fee25e4db5aaef54b312f97a28 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bo Yang Hong Liu Qin-Wen Xu Yi-Fan Sun Sui Xu Hao Zhang Jian-Xia Tang Jian-Xia Tang Guo-Ding Zhu Guo-Ding Zhu Yao-Bao Liu Yao-Bao Liu Jun Cao Jun Cao Jun Cao Yang Cheng |
spellingShingle |
Bo Yang Hong Liu Qin-Wen Xu Yi-Fan Sun Sui Xu Hao Zhang Jian-Xia Tang Jian-Xia Tang Guo-Ding Zhu Guo-Ding Zhu Yao-Bao Liu Yao-Bao Liu Jun Cao Jun Cao Jun Cao Yang Cheng Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China Frontiers in Genetics Plasmodium falciparum PfSRA genetic diversity imported malaria cases DNA sequencing Jiangsu Province |
author_facet |
Bo Yang Hong Liu Qin-Wen Xu Yi-Fan Sun Sui Xu Hao Zhang Jian-Xia Tang Jian-Xia Tang Guo-Ding Zhu Guo-Ding Zhu Yao-Bao Liu Yao-Bao Liu Jun Cao Jun Cao Jun Cao Yang Cheng |
author_sort |
Bo Yang |
title |
Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China |
title_short |
Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China |
title_full |
Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China |
title_fullStr |
Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China |
title_full_unstemmed |
Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to China |
title_sort |
genetic diversity analysis of surface-related antigen (sra) in plasmodium falciparum imported from africa to china |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Genetics |
issn |
1664-8021 |
publishDate |
2021-08-01 |
description |
Plasmodium falciparum surface-related antigen (SRA) is located on the surfaces of gametocyte and merozoite and has the structural and functional characteristics of potential targets for multistage vaccine development. However, little information is available regarding the genetic polymorphism of pfsra. To determine the extent of genetic variation about P. falciparum by characterizing the sra sequence, 74 P. falciparum samples were collected from migrant workers who returned to China from 12 countries of Africa between 2015 and 2019. The full length of the sra gene was amplified and sequenced. The average pairwise nucleotide diversities (π) of P. falciparum sra gene was 0.00132, and the haplotype diversity (Hd) was 0.770. The average number of nucleotide differences (k) for pfsra was 3.049. The ratio of non-synonymous (dN) to synonymous (dS) substitutions across sites (dN/dS) was 1.365. Amino acid substitutions of P. falciparum SRA could be categorized into 35 unique amino acid variants. Neutrality tests showed that the polymorphism of PfSRA was maintained by positive diversifying selection, which indicated its role as a potential target of protective immune responses and a vaccine candidate. Overall, the ability of the N-terminal of PfSRA antibodies to evoke inhibition of merozoite invasion of erythrocytes and conserved amino acid at low genetic diversity suggest that the N-terminal of PfSRA could be evaluated as a vaccine candidate against P. falciparum infection. |
topic |
Plasmodium falciparum PfSRA genetic diversity imported malaria cases DNA sequencing Jiangsu Province |
url |
https://www.frontiersin.org/articles/10.3389/fgene.2021.688606/full |
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doaj-08bbd5fee25e4db5aaef54b312f97a282021-08-05T16:02:36ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-08-011210.3389/fgene.2021.688606688606Genetic Diversity Analysis of Surface-Related Antigen (SRA) in Plasmodium falciparum Imported From Africa to ChinaBo Yang0Hong Liu1Qin-Wen Xu2Yi-Fan Sun3Sui Xu4Hao Zhang5Jian-Xia Tang6Jian-Xia Tang7Guo-Ding Zhu8Guo-Ding Zhu9Yao-Bao Liu10Yao-Bao Liu11Jun Cao12Jun Cao13Jun Cao14Yang Cheng15Laboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaLaboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, ChinaLaboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, ChinaKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasite Diseases, Wuxi, ChinaLaboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasite Diseases, Wuxi, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasite Diseases, Wuxi, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasite Diseases, Wuxi, ChinaLaboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing, ChinaKey Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasite Diseases, Wuxi, ChinaLaboratory of Pathogen Infection and Immunity, Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, ChinaPlasmodium falciparum surface-related antigen (SRA) is located on the surfaces of gametocyte and merozoite and has the structural and functional characteristics of potential targets for multistage vaccine development. However, little information is available regarding the genetic polymorphism of pfsra. To determine the extent of genetic variation about P. falciparum by characterizing the sra sequence, 74 P. falciparum samples were collected from migrant workers who returned to China from 12 countries of Africa between 2015 and 2019. The full length of the sra gene was amplified and sequenced. The average pairwise nucleotide diversities (π) of P. falciparum sra gene was 0.00132, and the haplotype diversity (Hd) was 0.770. The average number of nucleotide differences (k) for pfsra was 3.049. The ratio of non-synonymous (dN) to synonymous (dS) substitutions across sites (dN/dS) was 1.365. Amino acid substitutions of P. falciparum SRA could be categorized into 35 unique amino acid variants. Neutrality tests showed that the polymorphism of PfSRA was maintained by positive diversifying selection, which indicated its role as a potential target of protective immune responses and a vaccine candidate. Overall, the ability of the N-terminal of PfSRA antibodies to evoke inhibition of merozoite invasion of erythrocytes and conserved amino acid at low genetic diversity suggest that the N-terminal of PfSRA could be evaluated as a vaccine candidate against P. falciparum infection.https://www.frontiersin.org/articles/10.3389/fgene.2021.688606/fullPlasmodium falciparumPfSRAgenetic diversityimported malaria casesDNA sequencingJiangsu Province |