Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos
Abstract Over the past decade, DNA barcoding has become a staple of low-cost molecular systematic investigations. The availability of universal primers and subsidized sequencing projects (PolarBOL, SharkBOL, SpongeBOL) have driven this popularity, often without appropriate investigation into the uti...
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doaj-08c348547e134350993c14871d9dce262020-12-08T00:40:29ZengNature Publishing GroupScientific Reports2045-23222017-07-017111110.1038/s41598-017-05261-9Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckosIan G. Brennan0Aaron M. Bauer1Ngo Van Tri2Yun-yu Wang3Wen-zhi Wang4Ya-Ping Zhang5Robert W. Murphy6Division of Ecology & Evolution, Research School of Biology, The Australian National UniversityDivision of Ecology & Evolution, Research School of Biology, The Australian National UniversityNational Key Laboratory, Institute of Tropical Biology, Vietnamese Academy of Sciences and TechnologyState Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Genetic Resources and Evolution State, and Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of SciencesAbstract Over the past decade, DNA barcoding has become a staple of low-cost molecular systematic investigations. The availability of universal primers and subsidized sequencing projects (PolarBOL, SharkBOL, SpongeBOL) have driven this popularity, often without appropriate investigation into the utility of barcoding data for the taxonomic group of interest. Here, our primary aim is to determine the phylogenetic value of DNA barcoding (mitochondrial locus COI) within the gecko genus Cyrtodactylus. With >40 new species described since last systematic investigation, Cyrtodactylus represents one of the most diverse extant squamate genera, and their contemporary distribution spans the Indian subcontinent, eastward through Indochina, and into AustraloPapua. The complex biogeographic history of this group, and morphology-only designation of many species have complicated our phylogenetic understanding of Cyrtodactylus. To highlight the need for continued inclusive molecular assessment, we use Vietnamese Cyrtodactylus as a case study showing the geopolitically paraphyletic nature of their history. We compare COI to the legacy marker ND2, and discuss the value of COI as an interspecific marker, as well as its shortcomings at deeper evolutionary scales. We draw attention back to the Cold Code as a subsidized method for incorporating molecular methods into species descriptions in the effort to maintain accurate phylogenies.https://doi.org/10.1038/s41598-017-05261-9 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ian G. Brennan Aaron M. Bauer Ngo Van Tri Yun-yu Wang Wen-zhi Wang Ya-Ping Zhang Robert W. Murphy |
spellingShingle |
Ian G. Brennan Aaron M. Bauer Ngo Van Tri Yun-yu Wang Wen-zhi Wang Ya-Ping Zhang Robert W. Murphy Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos Scientific Reports |
author_facet |
Ian G. Brennan Aaron M. Bauer Ngo Van Tri Yun-yu Wang Wen-zhi Wang Ya-Ping Zhang Robert W. Murphy |
author_sort |
Ian G. Brennan |
title |
Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos |
title_short |
Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos |
title_full |
Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos |
title_fullStr |
Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos |
title_full_unstemmed |
Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos |
title_sort |
barcoding utility in a mega-diverse, cross-continental genus: keeping pace with cyrtodactylus geckos |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-07-01 |
description |
Abstract Over the past decade, DNA barcoding has become a staple of low-cost molecular systematic investigations. The availability of universal primers and subsidized sequencing projects (PolarBOL, SharkBOL, SpongeBOL) have driven this popularity, often without appropriate investigation into the utility of barcoding data for the taxonomic group of interest. Here, our primary aim is to determine the phylogenetic value of DNA barcoding (mitochondrial locus COI) within the gecko genus Cyrtodactylus. With >40 new species described since last systematic investigation, Cyrtodactylus represents one of the most diverse extant squamate genera, and their contemporary distribution spans the Indian subcontinent, eastward through Indochina, and into AustraloPapua. The complex biogeographic history of this group, and morphology-only designation of many species have complicated our phylogenetic understanding of Cyrtodactylus. To highlight the need for continued inclusive molecular assessment, we use Vietnamese Cyrtodactylus as a case study showing the geopolitically paraphyletic nature of their history. We compare COI to the legacy marker ND2, and discuss the value of COI as an interspecific marker, as well as its shortcomings at deeper evolutionary scales. We draw attention back to the Cold Code as a subsidized method for incorporating molecular methods into species descriptions in the effort to maintain accurate phylogenies. |
url |
https://doi.org/10.1038/s41598-017-05261-9 |
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