Ehrlichia canis in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phases
Abstract Background Concerns for recrudescence of Ehrlichia canis infection arise when immunosuppressive drugs are used to treat immune‐mediated diseases in dogs previously infected with E. canis. Objectives Determine whether administration of prednisolone and cyclosporine would reactivate E. canis...
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doaj-08cdbde262eb4bd8a69e0bf47b450b702020-11-25T02:53:42ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762020-05-013431214122110.1111/jvim.15750Ehrlichia canis in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phasesMasahiko Sato0Julia K. Veir1Sarah B. Shropshire2Michael R. Lappin3Department of Clinical Sciences Colorado State University Fort Collins Colorado USADepartment of Clinical Sciences Colorado State University Fort Collins Colorado USADepartment of Clinical Sciences Colorado State University Fort Collins Colorado USADepartment of Clinical Sciences Colorado State University Fort Collins Colorado USAAbstract Background Concerns for recrudescence of Ehrlichia canis infection arise when immunosuppressive drugs are used to treat immune‐mediated diseases in dogs previously infected with E. canis. Objectives Determine whether administration of prednisolone and cyclosporine would reactivate E. canis infection in dogs previously treated with doxycycline during the acute or subclinical phases. Animals Seven beagles previously experimentally infected with E. canis and administered doxycycline for 4 weeks were included. Three of the 7 dogs were incidentally concurrently infected with Anaplasma platys and Babesia vogeli and were administered 2 doses of imidocarb 2 weeks apart before enrollment in the current study. Methods Experimental study. Each dog was administered prednisolone and cyclosporine for 6 weeks. Clinical signs, complete blood cell count (CBC), polymerase chain reaction (PCR) assays for E. canis, A. platys, and B. vogeli DNA in blood, E. canis indirect fluorescent antibodies (IFA) titers, and flow cytometry for antiplatelet antibodies were monitored. Results All dogs completed the immunosuppressive protocol. No evidence for recrudescence of E. canis, A. platys, or B. vogeli were detected based on clinical signs or results of CBC, PCR, IFA, and flow cytometry for antiplatelet antibodies. E. canis IFA titers were negative in 5/7 dogs at the end of immunosuppressive protocol and were negative 6 months after the protocol in 5/5 dogs available for testing. Conclusions and Clinical Importance Dogs administered with a 4‐week course of doxycycline with or without imidocarb failed to show evidence of activation of E. canis infection after administration of a commonly used immune suppressive protocol.https://doi.org/10.1111/jvim.15750Anaplasma platysBabesia vogelicyclosporineEhrlichia canisprednisolone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Masahiko Sato Julia K. Veir Sarah B. Shropshire Michael R. Lappin |
spellingShingle |
Masahiko Sato Julia K. Veir Sarah B. Shropshire Michael R. Lappin Ehrlichia canis in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phases Journal of Veterinary Internal Medicine Anaplasma platys Babesia vogeli cyclosporine Ehrlichia canis prednisolone |
author_facet |
Masahiko Sato Julia K. Veir Sarah B. Shropshire Michael R. Lappin |
author_sort |
Masahiko Sato |
title |
Ehrlichia canis in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phases |
title_short |
Ehrlichia canis in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phases |
title_full |
Ehrlichia canis in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phases |
title_fullStr |
Ehrlichia canis in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phases |
title_full_unstemmed |
Ehrlichia canis in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phases |
title_sort |
ehrlichia canis in dogs experimentally infected, treated, and then immune suppressed during the acute or subclinical phases |
publisher |
Wiley |
series |
Journal of Veterinary Internal Medicine |
issn |
0891-6640 1939-1676 |
publishDate |
2020-05-01 |
description |
Abstract Background Concerns for recrudescence of Ehrlichia canis infection arise when immunosuppressive drugs are used to treat immune‐mediated diseases in dogs previously infected with E. canis. Objectives Determine whether administration of prednisolone and cyclosporine would reactivate E. canis infection in dogs previously treated with doxycycline during the acute or subclinical phases. Animals Seven beagles previously experimentally infected with E. canis and administered doxycycline for 4 weeks were included. Three of the 7 dogs were incidentally concurrently infected with Anaplasma platys and Babesia vogeli and were administered 2 doses of imidocarb 2 weeks apart before enrollment in the current study. Methods Experimental study. Each dog was administered prednisolone and cyclosporine for 6 weeks. Clinical signs, complete blood cell count (CBC), polymerase chain reaction (PCR) assays for E. canis, A. platys, and B. vogeli DNA in blood, E. canis indirect fluorescent antibodies (IFA) titers, and flow cytometry for antiplatelet antibodies were monitored. Results All dogs completed the immunosuppressive protocol. No evidence for recrudescence of E. canis, A. platys, or B. vogeli were detected based on clinical signs or results of CBC, PCR, IFA, and flow cytometry for antiplatelet antibodies. E. canis IFA titers were negative in 5/7 dogs at the end of immunosuppressive protocol and were negative 6 months after the protocol in 5/5 dogs available for testing. Conclusions and Clinical Importance Dogs administered with a 4‐week course of doxycycline with or without imidocarb failed to show evidence of activation of E. canis infection after administration of a commonly used immune suppressive protocol. |
topic |
Anaplasma platys Babesia vogeli cyclosporine Ehrlichia canis prednisolone |
url |
https://doi.org/10.1111/jvim.15750 |
work_keys_str_mv |
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