A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure

A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure

Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.

Bibliographic Details
Main Authors: Sarah A. Nordeen, Kasper R. Andersen, Kevin E. Knockenhauer, Jessica R. Ingram, Hidde L. Ploegh, Thomas U. Schwartz
Format: Article
Language:English
Published: Nature Publishing Group 2020-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19884-6
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spelling doaj-08cecb6773264203972c596074acd9d42021-05-11T07:58:07ZengNature Publishing GroupNature Communications2041-17232020-12-0111111310.1038/s41467-020-19884-6A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structureSarah A. Nordeen0Kasper R. Andersen1Kevin E. Knockenhauer2Jessica R. Ingram3Hidde L. Ploegh4Thomas U. Schwartz5Department of Biology, Massachusetts Institute of TechnologyDepartment of Biology, Massachusetts Institute of TechnologyDepartment of Biology, Massachusetts Institute of TechnologyBoston Children’s Hospital and Harvard Medical SchoolBoston Children’s Hospital and Harvard Medical SchoolDepartment of Biology, Massachusetts Institute of TechnologyCharacterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.https://doi.org/10.1038/s41467-020-19884-6
collection DOAJ
language English
format Article
sources DOAJ
author Sarah A. Nordeen
Kasper R. Andersen
Kevin E. Knockenhauer
Jessica R. Ingram
Hidde L. Ploegh
Thomas U. Schwartz
spellingShingle Sarah A. Nordeen
Kasper R. Andersen
Kevin E. Knockenhauer
Jessica R. Ingram
Hidde L. Ploegh
Thomas U. Schwartz
A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
Nature Communications
author_facet Sarah A. Nordeen
Kasper R. Andersen
Kevin E. Knockenhauer
Jessica R. Ingram
Hidde L. Ploegh
Thomas U. Schwartz
author_sort Sarah A. Nordeen
title A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_short A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_full A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_fullStr A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_full_unstemmed A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_sort nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-12-01
description Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.
url https://doi.org/10.1038/s41467-020-19884-6
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