A heritable antiviral RNAi response limits Orsay virus infection in Caenorhabditis elegans N2.
Orsay virus (OrV) is the first virus known to be able to complete a full infection cycle in the model nematode species Caenorhabditis elegans. OrV is transmitted horizontally and its infection is limited by antiviral RNA interference (RNAi). However, we have no insight into the kinetics of OrV repli...
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doaj-08ff4a90e544447f92c067f0b16be5702020-11-25T01:52:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8976010.1371/journal.pone.0089760A heritable antiviral RNAi response limits Orsay virus infection in Caenorhabditis elegans N2.Mark G SterkenL Basten SnoekKobus J BosmanJikke DaamenJoost A G RiksenJaap BakkerGorben P PijlmanJan E KammengaOrsay virus (OrV) is the first virus known to be able to complete a full infection cycle in the model nematode species Caenorhabditis elegans. OrV is transmitted horizontally and its infection is limited by antiviral RNA interference (RNAi). However, we have no insight into the kinetics of OrV replication in C. elegans. We developed an assay that infects worms in liquid, allowing precise monitoring of the infection. The assay revealed a dual role for the RNAi response in limiting Orsay virus infection in C. elegans. Firstly, it limits the progression of the initial infection at the step of recognition of dsRNA. Secondly, it provides an inherited protection against infection in the offspring. This establishes the heritable RNAi response as anti-viral mechanism during OrV infections in C. elegans. Our results further illustrate that the inheritance of the anti-viral response is important in controlling the infection in the canonical wild type Bristol N2. The OrV replication kinetics were established throughout the worm life-cycle, setting a standard for further quantitative assays with the OrV-C. elegans infection model.http://europepmc.org/articles/PMC3933659?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mark G Sterken L Basten Snoek Kobus J Bosman Jikke Daamen Joost A G Riksen Jaap Bakker Gorben P Pijlman Jan E Kammenga |
spellingShingle |
Mark G Sterken L Basten Snoek Kobus J Bosman Jikke Daamen Joost A G Riksen Jaap Bakker Gorben P Pijlman Jan E Kammenga A heritable antiviral RNAi response limits Orsay virus infection in Caenorhabditis elegans N2. PLoS ONE |
author_facet |
Mark G Sterken L Basten Snoek Kobus J Bosman Jikke Daamen Joost A G Riksen Jaap Bakker Gorben P Pijlman Jan E Kammenga |
author_sort |
Mark G Sterken |
title |
A heritable antiviral RNAi response limits Orsay virus infection in Caenorhabditis elegans N2. |
title_short |
A heritable antiviral RNAi response limits Orsay virus infection in Caenorhabditis elegans N2. |
title_full |
A heritable antiviral RNAi response limits Orsay virus infection in Caenorhabditis elegans N2. |
title_fullStr |
A heritable antiviral RNAi response limits Orsay virus infection in Caenorhabditis elegans N2. |
title_full_unstemmed |
A heritable antiviral RNAi response limits Orsay virus infection in Caenorhabditis elegans N2. |
title_sort |
heritable antiviral rnai response limits orsay virus infection in caenorhabditis elegans n2. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Orsay virus (OrV) is the first virus known to be able to complete a full infection cycle in the model nematode species Caenorhabditis elegans. OrV is transmitted horizontally and its infection is limited by antiviral RNA interference (RNAi). However, we have no insight into the kinetics of OrV replication in C. elegans. We developed an assay that infects worms in liquid, allowing precise monitoring of the infection. The assay revealed a dual role for the RNAi response in limiting Orsay virus infection in C. elegans. Firstly, it limits the progression of the initial infection at the step of recognition of dsRNA. Secondly, it provides an inherited protection against infection in the offspring. This establishes the heritable RNAi response as anti-viral mechanism during OrV infections in C. elegans. Our results further illustrate that the inheritance of the anti-viral response is important in controlling the infection in the canonical wild type Bristol N2. The OrV replication kinetics were established throughout the worm life-cycle, setting a standard for further quantitative assays with the OrV-C. elegans infection model. |
url |
http://europepmc.org/articles/PMC3933659?pdf=render |
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