Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathwayResearch in context
Background: Ulcerative Colitis (UC) is an Inflammatory Bowel Disease (IBD) characterized by uncontrolled immune response, diarrhoea, weight loss and bloody stools, where sustained remission is not currently achievable. Dextran Sulphate Sodium (DSS)-induced colitis is an animal model that closely mim...
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doaj-090b27d76aea4396a4eb13e3d790ccff2020-11-25T01:49:08ZengElsevierEBioMedicine2352-39642019-07-0145495510Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathwayResearch in contextNatalia Schneider Nunes0Parwathy Chandran1Maggie Sundby2Fernanda Visioli3Fabiany da Costa Gonçalves4Scott Robert Burks5Ana Helena Paz6Joseph Alan Frank7Frank Laboratory, Radiology and Imaging Sciences, Clinical Centre, NIH, Bethesda, MD, United States; Gastroenterology and Hepatology Sciences Graduate Program, UFRGS, Porto Alegre, RS, Brazil; Corresponding author at: Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bldg. 10 Room B1N256, 10 Center Drive, Bethesda, MD 20892, United States.Frank Laboratory, Radiology and Imaging Sciences, Clinical Centre, NIH, Bethesda, MD, United StatesFrank Laboratory, Radiology and Imaging Sciences, Clinical Centre, NIH, Bethesda, MD, United StatesFaculty of Dentistry, Oral Pathology, UFRGS, Porto Alegre, RS, BrazilExperimental Research Centre, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, BrazilFrank Laboratory, Radiology and Imaging Sciences, Clinical Centre, NIH, Bethesda, MD, United StatesGastroenterology and Hepatology Sciences Graduate Program, UFRGS, Porto Alegre, RS, BrazilFrank Laboratory, Radiology and Imaging Sciences, Clinical Centre, NIH, Bethesda, MD, United States; National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD, United StatesBackground: Ulcerative Colitis (UC) is an Inflammatory Bowel Disease (IBD) characterized by uncontrolled immune response, diarrhoea, weight loss and bloody stools, where sustained remission is not currently achievable. Dextran Sulphate Sodium (DSS)-induced colitis is an animal model that closely mimics human UC. Ultrasound (US) has been shown to prevent experimental acute kidney injury through vagus nerve (VN) stimulation and activation of the cholinergic anti-inflammatory pathway (CAIP). Since IBD patients may present dysfunctional VN activity, our aim was to determine the effects of therapeutic ultrasound (TUS) in DSS-induced colitis. Methods: Acute colitis was induced by 2% DSS in drinking water for 7 days and TUS was administered to the abdominal area for 7 min/day from days 4–10. Clinical symptoms were analysed, and biological samples were collected for proteomics, macroscopic and microscopic analysis, flow cytometry and immunohistochemistry. Findings: TUS attenuated colitis by reducing clinical scores, colon shortening and histological damage, inducing proteomic tolerogenic response in the gut during the injury phase and early recovery of experimental colitis. TUS did not improve clinical and pathological outcomes in splenectomised mice, while α7nAChR (α7 nicotinic acetylcholine receptor - indicator of CAIP involvement) knockout animals presented with disease worsening. Increased levels of colonic F4/80+α7nAChR+ macrophages in wild type mice suggest CAIP activation. Interpretation: These results indicate TUS improved DSS-induced colitis through stimulation of the splenic nerve along with possible contribution by VN with CAIP activation. Fund: Intramural Research Programs of the Clinical Centre, the National Institute of Biomedical Imaging and Bioengineering at the NIH and CAPES/Brazil. Keywords: Therapeutic ultrasound, Dextran Sulphate sodium, Cholinergic anti-inflammatory pathway, Inflammatory bowel disease, Acute ulcerative colitis, α7 nicotinic acetylcholine receptorhttp://www.sciencedirect.com/science/article/pii/S2352396419304141 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Natalia Schneider Nunes Parwathy Chandran Maggie Sundby Fernanda Visioli Fabiany da Costa Gonçalves Scott Robert Burks Ana Helena Paz Joseph Alan Frank |
spellingShingle |
Natalia Schneider Nunes Parwathy Chandran Maggie Sundby Fernanda Visioli Fabiany da Costa Gonçalves Scott Robert Burks Ana Helena Paz Joseph Alan Frank Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathwayResearch in context EBioMedicine |
author_facet |
Natalia Schneider Nunes Parwathy Chandran Maggie Sundby Fernanda Visioli Fabiany da Costa Gonçalves Scott Robert Burks Ana Helena Paz Joseph Alan Frank |
author_sort |
Natalia Schneider Nunes |
title |
Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathwayResearch in context |
title_short |
Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathwayResearch in context |
title_full |
Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathwayResearch in context |
title_fullStr |
Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathwayResearch in context |
title_full_unstemmed |
Therapeutic ultrasound attenuates DSS-induced colitis through the cholinergic anti-inflammatory pathwayResearch in context |
title_sort |
therapeutic ultrasound attenuates dss-induced colitis through the cholinergic anti-inflammatory pathwayresearch in context |
publisher |
Elsevier |
series |
EBioMedicine |
issn |
2352-3964 |
publishDate |
2019-07-01 |
description |
Background: Ulcerative Colitis (UC) is an Inflammatory Bowel Disease (IBD) characterized by uncontrolled immune response, diarrhoea, weight loss and bloody stools, where sustained remission is not currently achievable. Dextran Sulphate Sodium (DSS)-induced colitis is an animal model that closely mimics human UC. Ultrasound (US) has been shown to prevent experimental acute kidney injury through vagus nerve (VN) stimulation and activation of the cholinergic anti-inflammatory pathway (CAIP). Since IBD patients may present dysfunctional VN activity, our aim was to determine the effects of therapeutic ultrasound (TUS) in DSS-induced colitis. Methods: Acute colitis was induced by 2% DSS in drinking water for 7 days and TUS was administered to the abdominal area for 7 min/day from days 4–10. Clinical symptoms were analysed, and biological samples were collected for proteomics, macroscopic and microscopic analysis, flow cytometry and immunohistochemistry. Findings: TUS attenuated colitis by reducing clinical scores, colon shortening and histological damage, inducing proteomic tolerogenic response in the gut during the injury phase and early recovery of experimental colitis. TUS did not improve clinical and pathological outcomes in splenectomised mice, while α7nAChR (α7 nicotinic acetylcholine receptor - indicator of CAIP involvement) knockout animals presented with disease worsening. Increased levels of colonic F4/80+α7nAChR+ macrophages in wild type mice suggest CAIP activation. Interpretation: These results indicate TUS improved DSS-induced colitis through stimulation of the splenic nerve along with possible contribution by VN with CAIP activation. Fund: Intramural Research Programs of the Clinical Centre, the National Institute of Biomedical Imaging and Bioengineering at the NIH and CAPES/Brazil. Keywords: Therapeutic ultrasound, Dextran Sulphate sodium, Cholinergic anti-inflammatory pathway, Inflammatory bowel disease, Acute ulcerative colitis, α7 nicotinic acetylcholine receptor |
url |
http://www.sciencedirect.com/science/article/pii/S2352396419304141 |
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