Uji Larvasida Crude Protease Getah Widuri (Calotropis gigantea) Terhadap Larva Nyamuk Aedes aegypti

• Main Authors: Meiske Elisabeth Koraag, Rina Isnawati, Ade Kurniawan, Risti Risti, Nurul Hidayah
• Format: Article
• Language: English
• Published: Badan Penelitian dan Pengembangan Kesehatan 2017-12-01
• Series: Jurnal Vektor Penyakit
• Subjects: crude protease,Calotropis gigantea, Ae. aegypti, larva
• Online Access: http://ejournal.litbang.depkes.go.id/index.php/vektorp/article/view/7342

<p>The using of chemical insecticide such as temephos and malathion can cause problems for the environment as well as resistance to mosquitoes. These insecticide residues will be accumulated and difficult to be degraded in the environment. Alternatively, plants that have potential active chemical compund as larvacides should be developed. This study aims to know the lethal concentration of the crude protease from widuri latex againts <em>Aedes aegypti</em> larvae. This was an experimental study which the sample size was determined by using the Frederer formula. The study used five different concentrations and five repetitions. There were two controls group, positive and negative control. Positive control used <em>Bacillus thuringiensis </em>and negative control used water. The results showed that the LC50 and LC90 of crude protease before storage were 352,34 ppm and 673,40 ppm, respectively while crude protease has been stored for one week were 778,32 ppm and 1399,33 ppm, respectively. Crude protease before storage is more effective than crude protease after stored in killing <em>Ae. aegypti </em>larvae.</p><p> </p><p>Penggunaan insektisida kimia seperti temephos dan malathion dapat menimbulkan masalah bagi lingkungan dan juga resistensi pada nyamuk. Residu insektisida ini akan terakumulasi dan sukar terdegradasi di lingkungan. Insektisida alternatif perlu dikembangkan menggunakan tanaman yang memiliki senyawa kimia aktif yang potensial sebagai larvasida. Penelitian ini bertujuan untuk menentukan daya bunuhcrude protease getah widuri terhadap larva nyamuk <em>Ae. aegypti</em>. Jenis penelitian yang digunakan yaitu eksperimental murni, besar sampel ditentukan berdasarkan rumus federer diperoleh lima konsentrasi perlakuan dan lima pengulangan. Terdapat dua kelompok kontrol yaitu kontrol positif dan kontrol negatif. Kontrol positif menggunakan <em>Bacillus thuringiensis </em>dan kontrol negatif menggunakan air. Hasilpenelitian menunjukkan bahwa pada uji akhir untuk <em>crude protease</em> getah widuri sebelum dilakukan penyimpanan diperoleh nilai LC sebesar 352,34 ppm dan LC 50 90sebesar 673,40 ppm, sedangkan setelah dilakukan penyimpanan selama satu minggu diperoleh LC sebesar 778,32 ppm dan LC sebesar 1399,33 ppm. Daya bunuh <em>crude protease</em> getah widuri sebelum penyimpanan lebih efektif dibandingkan setelah dilakukan penyimpanan selama satu minggu.</p>