VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER

• Main Authors: M. RIZAL, HERDIS, YULNAWATI, H. MAHESHWARI
• Format: Article
• Language: English
• Published: SEAMEO, BIOTROP 2009-01-01
• Series: Biotropia: The Southeast Asian Journal of Tropical Biology
• Subjects: epididymal sperm; cryopreservation; dextrose; spotted buffalo
• Online Access: http://journal.biotrop.org/index.php/biotropia/article/view/63/42

h e objective of this study was to obtain the viability and plasma membrane integrity of the spotted buff alo epididymal sperm after addition of dextrose into Andromed® extender. Spermatozoa that have been collected from cauda epididymis were diluted with Andromed® extender as control (K) and Andromed® + 0.2% dextrose (P1) and Andromed® + 0.4% dextrose (P2) as treatments. h e results showed that the quality of epididymal spermatozoa decreased during cryopreservation process. h e percentage of motility after thawing in P1 (46%) and P2 (46.67%) were signifi cantly higher (P<0.05) compared to K (41%) as well as the percentage of live sperm in P1 (58.8%) and P2 (60%) compared to K (52.2%). h e percentage of membrane integrity in P1, P2 and K were 67.4; 66.8 and 68 %, respectively. In conclusion, the addition of 0.2 and 0.4% of dextrose into Andromed® acted as an extra cellular cryoprotectant and could maintain the viability and membrane integrity of the spotted buff alo epididymal spermatozoa after thawing.