VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER
h e objective of this study was to obtain the viability and plasma membrane integrity of the spotted buff alo epididymal sperm after addition of dextrose into Andromed® extender. Spermatozoa that have been collected from cauda epididymis were diluted with Andromed® extender as control (K) and Andr...
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SEAMEO, BIOTROP
2009-01-01
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doaj-090ff79a9cac49fbb67cf5248ec3242c2020-11-24T23:46:58ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2009-01-011612127VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDERM. RIZALHERDISYULNAWATIH. MAHESHWARIh e objective of this study was to obtain the viability and plasma membrane integrity of the spotted buff alo epididymal sperm after addition of dextrose into Andromed® extender. Spermatozoa that have been collected from cauda epididymis were diluted with Andromed® extender as control (K) and Andromed® + 0.2% dextrose (P1) and Andromed® + 0.4% dextrose (P2) as treatments. h e results showed that the quality of epididymal spermatozoa decreased during cryopreservation process. h e percentage of motility after thawing in P1 (46%) and P2 (46.67%) were signifi cantly higher (P<0.05) compared to K (41%) as well as the percentage of live sperm in P1 (58.8%) and P2 (60%) compared to K (52.2%). h e percentage of membrane integrity in P1, P2 and K were 67.4; 66.8 and 68 %, respectively. In conclusion, the addition of 0.2 and 0.4% of dextrose into Andromed® acted as an extra cellular cryoprotectant and could maintain the viability and membrane integrity of the spotted buff alo epididymal spermatozoa after thawing.http://journal.biotrop.org/index.php/biotropia/article/view/63/42epididymal spermcryopreservationdextrosespotted buffalo |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. RIZAL HERDIS YULNAWATI H. MAHESHWARI |
spellingShingle |
M. RIZAL HERDIS YULNAWATI H. MAHESHWARI VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER Biotropia: The Southeast Asian Journal of Tropical Biology epididymal sperm cryopreservation dextrose spotted buffalo |
author_facet |
M. RIZAL HERDIS YULNAWATI H. MAHESHWARI |
author_sort |
M. RIZAL |
title |
VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER |
title_short |
VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER |
title_full |
VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER |
title_fullStr |
VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER |
title_full_unstemmed |
VIABILITY AND PLASMA MEMBRANE INTEGRITY OF THE SPOTTED BUFFALO EPIDIDYMAL SPERMATOZOA AFTER THAWING WITH THE ADDITION OF DEXTROSE INTO THE EXTENDER |
title_sort |
viability and plasma membrane integrity of the spotted buffalo epididymal spermatozoa after thawing with the addition of dextrose into the extender |
publisher |
SEAMEO, BIOTROP |
series |
Biotropia: The Southeast Asian Journal of Tropical Biology |
issn |
0215-6334 1907-770X |
publishDate |
2009-01-01 |
description |
h e objective of this study was to obtain the viability and plasma membrane integrity of the spotted buff alo epididymal sperm after addition of dextrose into Andromed® extender. Spermatozoa that have been collected from cauda epididymis were diluted with Andromed® extender as control (K) and Andromed® + 0.2% dextrose (P1) and Andromed® + 0.4% dextrose (P2) as treatments. h e results showed that the quality of epididymal spermatozoa decreased during cryopreservation process. h e percentage of motility after thawing in P1 (46%) and P2 (46.67%) were signifi cantly higher (P<0.05) compared to K (41%) as well as the percentage of live sperm in P1 (58.8%) and P2 (60%) compared to K (52.2%). h e percentage of membrane integrity in P1, P2 and K were 67.4; 66.8 and 68 %, respectively. In conclusion, the addition of 0.2 and 0.4% of dextrose into Andromed® acted as an extra cellular cryoprotectant and could maintain the viability and membrane integrity of the spotted buff alo epididymal spermatozoa after thawing. |
topic |
epididymal sperm cryopreservation dextrose spotted buffalo |
url |
http://journal.biotrop.org/index.php/biotropia/article/view/63/42 |
work_keys_str_mv |
AT mrizal viabilityandplasmamembraneintegrityofthespottedbuffaloepididymalspermatozoaafterthawingwiththeadditionofdextroseintotheextender AT herdis viabilityandplasmamembraneintegrityofthespottedbuffaloepididymalspermatozoaafterthawingwiththeadditionofdextroseintotheextender AT yulnawati viabilityandplasmamembraneintegrityofthespottedbuffaloepididymalspermatozoaafterthawingwiththeadditionofdextroseintotheextender AT hmaheshwari viabilityandplasmamembraneintegrityofthespottedbuffaloepididymalspermatozoaafterthawingwiththeadditionofdextroseintotheextender |
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