Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells
This work shows the synthesis of a polyvinylpyrrolidone (PVP) hydrogel by heat-activated polymerization and explores the production of hydrogels with an open porous network by lyophilisation to allow the three-dimensional culture of human oral mucosa stem cells (hOMSCs). The swollen hydrogel showed...
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doaj-092a7c284d00481a8c91cb0c792e2e1c2021-01-06T00:03:13ZengMDPI AGMaterials1996-19442021-01-011422722710.3390/ma14010227Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem CellsCarolina Oliver-Urrutia0Raúl Rosales Ibañez1Miriam V. Flores-Merino2Lucy Vojtova3Jakub Salplachta4Ladislav Čelko5Jozef Kaiser6Edgar B. Montufar7Faculty of Chemistry, Autonomous University of the State of Mexico, Paseo Colon S/N, 50120 Toluca, MexicoFaculty of Higher Studies Iztacala, National Autonomous University of Mexico, Los Reyes Iztacala 1, 54090 Mexico City, MexicoFaculty of Chemistry, Autonomous University of the State of Mexico, Paseo Colon S/N, 50120 Toluca, MexicoCentral European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech RepublicCentral European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech RepublicCentral European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech RepublicCentral European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech RepublicCentral European Institute of Technology, Brno University of Technology, Purkynova 123, 61200 Brno, Czech RepublicThis work shows the synthesis of a polyvinylpyrrolidone (PVP) hydrogel by heat-activated polymerization and explores the production of hydrogels with an open porous network by lyophilisation to allow the three-dimensional culture of human oral mucosa stem cells (hOMSCs). The swollen hydrogel showed a storage modulus similar to oral mucosa and elastic solid rheological behaviour without sol transition. A comprehensive characterization of porosity by scanning electron microscopy, mercury intrusion porosimetry and nano-computed tomography (with spatial resolution below 1 μm) showed that lyophilisation resulted in the heterogeneous incorporation of closed oval-like pores in the hydrogel with broad size distribution (5 to 180 μm, d<sub>50</sub> = 65 μm). Human oral mucosa biopsies were used to isolate hOMSCs, expressing typical markers of mesenchymal stem cells in more than 95% of the cell population. Direct contact cytotoxicity assay demonstrated that PVP hydrogel have no negative effect on cell metabolic activity, allowing the culture of hOMSCs with normal fusiform morphology. Pore connectivity should be improved in future to allow cell growth in the bulk of the PVP hydrogel.https://www.mdpi.com/1996-1944/14/1/227polyvinylpyrrolidonehydrogellyophilisationnano-computed tomographyporosityoral mucosa |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carolina Oliver-Urrutia Raúl Rosales Ibañez Miriam V. Flores-Merino Lucy Vojtova Jakub Salplachta Ladislav Čelko Jozef Kaiser Edgar B. Montufar |
spellingShingle |
Carolina Oliver-Urrutia Raúl Rosales Ibañez Miriam V. Flores-Merino Lucy Vojtova Jakub Salplachta Ladislav Čelko Jozef Kaiser Edgar B. Montufar Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells Materials polyvinylpyrrolidone hydrogel lyophilisation nano-computed tomography porosity oral mucosa |
author_facet |
Carolina Oliver-Urrutia Raúl Rosales Ibañez Miriam V. Flores-Merino Lucy Vojtova Jakub Salplachta Ladislav Čelko Jozef Kaiser Edgar B. Montufar |
author_sort |
Carolina Oliver-Urrutia |
title |
Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells |
title_short |
Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells |
title_full |
Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells |
title_fullStr |
Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells |
title_full_unstemmed |
Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells |
title_sort |
lyophilized polyvinylpyrrolidone hydrogel for culture of human oral mucosa stem cells |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2021-01-01 |
description |
This work shows the synthesis of a polyvinylpyrrolidone (PVP) hydrogel by heat-activated polymerization and explores the production of hydrogels with an open porous network by lyophilisation to allow the three-dimensional culture of human oral mucosa stem cells (hOMSCs). The swollen hydrogel showed a storage modulus similar to oral mucosa and elastic solid rheological behaviour without sol transition. A comprehensive characterization of porosity by scanning electron microscopy, mercury intrusion porosimetry and nano-computed tomography (with spatial resolution below 1 μm) showed that lyophilisation resulted in the heterogeneous incorporation of closed oval-like pores in the hydrogel with broad size distribution (5 to 180 μm, d<sub>50</sub> = 65 μm). Human oral mucosa biopsies were used to isolate hOMSCs, expressing typical markers of mesenchymal stem cells in more than 95% of the cell population. Direct contact cytotoxicity assay demonstrated that PVP hydrogel have no negative effect on cell metabolic activity, allowing the culture of hOMSCs with normal fusiform morphology. Pore connectivity should be improved in future to allow cell growth in the bulk of the PVP hydrogel. |
topic |
polyvinylpyrrolidone hydrogel lyophilisation nano-computed tomography porosity oral mucosa |
url |
https://www.mdpi.com/1996-1944/14/1/227 |
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