Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males
Telomere length can be influenced by reactive oxygen species (ROS) generated by lifestyle factors or environmental exposure. We sought to determine whether oxidative stress has an impact on sperm nuclear alterations, especially on chromatin organization and telomere interactions in the spermatozoa o...
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doaj-0969e6f2e7ff4df5a574227d648a5ff72021-04-12T23:04:43ZengMDPI AGAntioxidants2076-39212021-04-011059359310.3390/antiox10040593Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile MalesBenoit Berby0Cynthia Bichara1Aurélie Rives-Feraille2Fanny Jumeau3Pierre Di Pizio4Véronique Sétif5Louis Sibert6Ludovic Dumont7Chistine Rondanino8Nathalie Rives9Biology of Reproduction—CECOS Laboratory, Rouen University Hospital, Normandie Univ, UNIROUEN, EA 4308 “Gametogenesis and Gamete Quality”, F 76000 Rouen, FranceBiology of Reproduction—CECOS Laboratory, Rouen University Hospital, Normandie Univ, UNIROUEN, EA 4308 “Gametogenesis and Gamete Quality”, F 76000 Rouen, FranceBiology of Reproduction—CECOS Laboratory, Rouen University Hospital, Normandie Univ, UNIROUEN, EA 4308 “Gametogenesis and Gamete Quality”, F 76000 Rouen, FranceBiology of Reproduction—CECOS Laboratory, Rouen University Hospital, Normandie Univ, UNIROUEN, EA 4308 “Gametogenesis and Gamete Quality”, F 76000 Rouen, FranceBiology of Reproduction—CECOS Laboratory, Rouen University Hospital, Normandie Univ, UNIROUEN, EA 4308 “Gametogenesis and Gamete Quality”, F 76000 Rouen, FranceBiology of Reproduction—CECOS Laboratory, Rouen University Hospital, Normandie Univ, UNIROUEN, EA 4308 “Gametogenesis and Gamete Quality”, F 76000 Rouen, FranceDepartment of Urology—Andrology, Rouen University Hospital, Normandie Univ, UNIROUEN, EA 4308 “Gametogenesis and Gamete Quality”, F 76000 Rouen, FranceEA 4308 “Gametogenesis and Gamete Quality”, Normandie University, UNIROUEN, F 76000 Rouen, FranceEA 4308 “Gametogenesis and Gamete Quality”, Normandie University, UNIROUEN, F 76000 Rouen, FranceBiology of Reproduction—CECOS Laboratory, Rouen University Hospital, Normandie Univ, UNIROUEN, EA 4308 “Gametogenesis and Gamete Quality”, F 76000 Rouen, FranceTelomere length can be influenced by reactive oxygen species (ROS) generated by lifestyle factors or environmental exposure. We sought to determine whether oxidative stress has an impact on sperm nuclear alterations, especially on chromatin organization and telomere interactions in the spermatozoa of infertile males. We performed an observational and prospective study including fifty-two males, allocated in the “case group” (30 infertile males presenting conventional semen parameter alterations) and the “control group” (22 males with normal conventional semen parameters). ROS detection was determined on spermatozoa using CellROX<sup>©</sup> probes. Sperm nuclear damage was assessed using quantitative fluorescence in situ hybridization (Q-FISH) for relative telomere length and telomere number, aniline blue staining for chromatin condensation, terminal deoxynucleotidyl transferase dUTP nick-end labeling for DNA fragmentation, and FISH for aneuploidy and 8-hydroxy-2′-deoxyguanosine immunostaining for oxidative DNA damages. Infertile males had significantly increased levels of cytoplasmic ROS and chromatin condensation defects as well as a higher mean number of telomere signals per spermatozoon in comparison with controls. In addition, the mean number of sperm telomere signals were positively correlated with the percentage of spermatozoa with chromatin condensation defect. In infertile males with conventional semen parameter alterations, oxidative stress is associated with telomere interaction impairment and chromatin condensation defects.https://www.mdpi.com/2076-3921/10/4/593chromatin condensationmale infertilityoxidative stressspermatozoatelomere |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Benoit Berby Cynthia Bichara Aurélie Rives-Feraille Fanny Jumeau Pierre Di Pizio Véronique Sétif Louis Sibert Ludovic Dumont Chistine Rondanino Nathalie Rives |
spellingShingle |
Benoit Berby Cynthia Bichara Aurélie Rives-Feraille Fanny Jumeau Pierre Di Pizio Véronique Sétif Louis Sibert Ludovic Dumont Chistine Rondanino Nathalie Rives Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males Antioxidants chromatin condensation male infertility oxidative stress spermatozoa telomere |
author_facet |
Benoit Berby Cynthia Bichara Aurélie Rives-Feraille Fanny Jumeau Pierre Di Pizio Véronique Sétif Louis Sibert Ludovic Dumont Chistine Rondanino Nathalie Rives |
author_sort |
Benoit Berby |
title |
Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males |
title_short |
Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males |
title_full |
Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males |
title_fullStr |
Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males |
title_full_unstemmed |
Oxidative Stress Is Associated with Telomere Interaction Impairment and Chromatin Condensation Defects in Spermatozoa of Infertile Males |
title_sort |
oxidative stress is associated with telomere interaction impairment and chromatin condensation defects in spermatozoa of infertile males |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2021-04-01 |
description |
Telomere length can be influenced by reactive oxygen species (ROS) generated by lifestyle factors or environmental exposure. We sought to determine whether oxidative stress has an impact on sperm nuclear alterations, especially on chromatin organization and telomere interactions in the spermatozoa of infertile males. We performed an observational and prospective study including fifty-two males, allocated in the “case group” (30 infertile males presenting conventional semen parameter alterations) and the “control group” (22 males with normal conventional semen parameters). ROS detection was determined on spermatozoa using CellROX<sup>©</sup> probes. Sperm nuclear damage was assessed using quantitative fluorescence in situ hybridization (Q-FISH) for relative telomere length and telomere number, aniline blue staining for chromatin condensation, terminal deoxynucleotidyl transferase dUTP nick-end labeling for DNA fragmentation, and FISH for aneuploidy and 8-hydroxy-2′-deoxyguanosine immunostaining for oxidative DNA damages. Infertile males had significantly increased levels of cytoplasmic ROS and chromatin condensation defects as well as a higher mean number of telomere signals per spermatozoon in comparison with controls. In addition, the mean number of sperm telomere signals were positively correlated with the percentage of spermatozoa with chromatin condensation defect. In infertile males with conventional semen parameter alterations, oxidative stress is associated with telomere interaction impairment and chromatin condensation defects. |
topic |
chromatin condensation male infertility oxidative stress spermatozoa telomere |
url |
https://www.mdpi.com/2076-3921/10/4/593 |
work_keys_str_mv |
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1721529332823752704 |