The impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilization
Abstract Background To evaluate the impact of follicle-flushing during oocyte collection on embryo development potential retrospectively. Methods A total of 1714 cases, including 133 who experienced retrieval difficulty (repeated follicle-flushing) on the day of oocyte retrieval (difficulty group) a...
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doaj-09705e0e496d465c9028a6e14ee9145e2020-12-06T12:11:14ZengBMCReproductive Biology and Endocrinology1477-78272019-12-011711710.1186/s12958-019-0540-5The impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilizationLi-hua Zhu0Xiao-bei Ni1Fei Lin2Zhi-peng Xu3Jun-shun Fang4Ning-yuan Zhang5Reproductive Medical Center, Drum Tower Hospital Affiliated with Nanjing University Medical CollegeReproductive Medical Center, Drum Tower Hospital Affiliated with Nanjing University Medical CollegeReproductive Medical Center, Drum Tower Hospital Affiliated with Nanjing University Medical CollegeReproductive Medical Center, Drum Tower Hospital Affiliated with Nanjing University Medical CollegeReproductive Medical Center, Drum Tower Hospital Affiliated with Nanjing University Medical CollegeReproductive Medical Center, Drum Tower Hospital Affiliated with Nanjing University Medical CollegeAbstract Background To evaluate the impact of follicle-flushing during oocyte collection on embryo development potential retrospectively. Methods A total of 1714 cases, including 133 who experienced retrieval difficulty (repeated follicle-flushing) on the day of oocyte retrieval (difficulty group) and the control 1581 cases (control group), were assessed in this retrospective study. The number of oocytes recovered, two pro-nuclei fertilization (2PN-fertilization), day 3 good-quality embryo and day 5/6 blastocyst utilization rates were compared between the difficulty group and control group correspondingly. Embryo implantation, clinical pregnancy and neonatal outcomes were further analyzed between the two groups in the fresh day− 3 embryo transfer cycles. Results The number of oocytes recovered in the difficulty group (9.08 ± 4.65) were significantly reduced compared with the control group (12.13 ± 5.27),P < 0.001; The 2PN-fertilization, day 3 good-quality embryo and blastocyst utilization rates were significantly lower in the difficulty group compared with controls (71.7% vs. 75.7%; 52.7% vs. 56.5%; 31.9% vs. 37.0%, all P < 0.05). Embryo implantation in the difficulty group was 53.2%, which was lower than the control value of 58.7%, although not reaching statistical significance. The rate of fresh embryo transfer cycles in the difficulty group was lower than normal ones (51.88% vs. 61.99%, P = 0.026). The pregnancy and live birth rates were similar between the two groups. But the rate of spontaneous miscarriages of the difficulty group was higher than the control group, although not reaching statistical significance. The neonatal outcomes had no statistical difference between the two groups. Conclusions Oocyte retrieval difficulty, which include repeated flushing and the corresponded extending time required for oocyte recovery, significantly reduced day 3 good-quality embryo and blastocyst utilization rates of these patients. But the live birth rate had no difference between the difficulty group and the normal ones.https://doi.org/10.1186/s12958-019-0540-5Oocyte retrieval difficultyNumber of recovered oocytesEmbryo development potentialBlastulation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li-hua Zhu Xiao-bei Ni Fei Lin Zhi-peng Xu Jun-shun Fang Ning-yuan Zhang |
spellingShingle |
Li-hua Zhu Xiao-bei Ni Fei Lin Zhi-peng Xu Jun-shun Fang Ning-yuan Zhang The impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilization Reproductive Biology and Endocrinology Oocyte retrieval difficulty Number of recovered oocytes Embryo development potential Blastulation |
author_facet |
Li-hua Zhu Xiao-bei Ni Fei Lin Zhi-peng Xu Jun-shun Fang Ning-yuan Zhang |
author_sort |
Li-hua Zhu |
title |
The impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilization |
title_short |
The impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilization |
title_full |
The impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilization |
title_fullStr |
The impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilization |
title_full_unstemmed |
The impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilization |
title_sort |
impact of follicle-flushing during oocyte collection on embryo development of in-vitro fertilization |
publisher |
BMC |
series |
Reproductive Biology and Endocrinology |
issn |
1477-7827 |
publishDate |
2019-12-01 |
description |
Abstract Background To evaluate the impact of follicle-flushing during oocyte collection on embryo development potential retrospectively. Methods A total of 1714 cases, including 133 who experienced retrieval difficulty (repeated follicle-flushing) on the day of oocyte retrieval (difficulty group) and the control 1581 cases (control group), were assessed in this retrospective study. The number of oocytes recovered, two pro-nuclei fertilization (2PN-fertilization), day 3 good-quality embryo and day 5/6 blastocyst utilization rates were compared between the difficulty group and control group correspondingly. Embryo implantation, clinical pregnancy and neonatal outcomes were further analyzed between the two groups in the fresh day− 3 embryo transfer cycles. Results The number of oocytes recovered in the difficulty group (9.08 ± 4.65) were significantly reduced compared with the control group (12.13 ± 5.27),P < 0.001; The 2PN-fertilization, day 3 good-quality embryo and blastocyst utilization rates were significantly lower in the difficulty group compared with controls (71.7% vs. 75.7%; 52.7% vs. 56.5%; 31.9% vs. 37.0%, all P < 0.05). Embryo implantation in the difficulty group was 53.2%, which was lower than the control value of 58.7%, although not reaching statistical significance. The rate of fresh embryo transfer cycles in the difficulty group was lower than normal ones (51.88% vs. 61.99%, P = 0.026). The pregnancy and live birth rates were similar between the two groups. But the rate of spontaneous miscarriages of the difficulty group was higher than the control group, although not reaching statistical significance. The neonatal outcomes had no statistical difference between the two groups. Conclusions Oocyte retrieval difficulty, which include repeated flushing and the corresponded extending time required for oocyte recovery, significantly reduced day 3 good-quality embryo and blastocyst utilization rates of these patients. But the live birth rate had no difference between the difficulty group and the normal ones. |
topic |
Oocyte retrieval difficulty Number of recovered oocytes Embryo development potential Blastulation |
url |
https://doi.org/10.1186/s12958-019-0540-5 |
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