GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton
Abstract Background The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the...
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doaj-0975404c8065483a894b4a9497fa46a12020-11-25T03:46:11ZengBMCBMC Plant Biology1471-22292019-08-0119111210.1186/s12870-019-1945-1GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cottonPeng He0Shuyin Wu1Yanli Jiang2Lihua Zhang3Meiju Tang4Guanghui Xiao5Jianing Yu6College of Life Sciences, Shaanxi Normal UniversityCollege of Life Sciences, Shaanxi Normal UniversityShanxi Academy of Agricultural Sciences, Cotton Research InstituteCollege of Life Sciences, Shaanxi Normal UniversityCollege of Life Sciences, Shaanxi Normal UniversityKey Laboratory of the Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in the Northwest of China, College of Life Sciences, Shaanxi Normal UniversityCollege of Life Sciences, Shaanxi Normal UniversityAbstract Background The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the function of PPR proteins in cotton is still unknown. Results In this study, we characterized a PPR gene YELLOW-GREEN LEAF (GhYGL1d) that is required for cotton plastid development. The GhYGL1d gene has a DYW domain in C-terminal and is highly express in leaves, localized to the chloroplast fractions. GhYGL1d share high amino acid-sequence homology with AtECB2. In atecb2 mutant, overexpression of GhYGL1d rescued the seedling lethal phenotype and restored the editing of accD and ndhF transcripts. Silencing of GhYGL1d led to the reduction of chlorophyll and phenotypically yellow-green leaves in cotton. Compared with wild type, GhYGL1d-silenced cotton showed significant deformations of thylakoid structures. Furthermore, the transcription levels of plastid-encoded polymerase (PEP) and nuclear-encoded polymerase (NEP) dependent genes were decreased in GhYGL1d-silenced cotton. Conclusions Our data indicate that GhYGL1d not only contributes to the editing of accD and ndhF genes, but also affects the expression of NEP- and PEP-dependent genes to regulate the development of thylakoids, and therefore regulates leaf variegation in cotton.http://link.springer.com/article/10.1186/s12870-019-1945-1ChloroplastPPRLeaf variegationCotton |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Peng He Shuyin Wu Yanli Jiang Lihua Zhang Meiju Tang Guanghui Xiao Jianing Yu |
spellingShingle |
Peng He Shuyin Wu Yanli Jiang Lihua Zhang Meiju Tang Guanghui Xiao Jianing Yu GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton BMC Plant Biology Chloroplast PPR Leaf variegation Cotton |
author_facet |
Peng He Shuyin Wu Yanli Jiang Lihua Zhang Meiju Tang Guanghui Xiao Jianing Yu |
author_sort |
Peng He |
title |
GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton |
title_short |
GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton |
title_full |
GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton |
title_fullStr |
GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton |
title_full_unstemmed |
GhYGL1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton |
title_sort |
ghygl1d, a pentatricopeptide repeat protein, is required for chloroplast development in cotton |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2019-08-01 |
description |
Abstract Background The pentatricopeptide repeat (PPR) gene family, which contains multiple 35-amino acid repeats, constitutes one of the largest gene families in plants. PPR proteins function in organelles to target specific transcripts and are involved in plant development and growth. However, the function of PPR proteins in cotton is still unknown. Results In this study, we characterized a PPR gene YELLOW-GREEN LEAF (GhYGL1d) that is required for cotton plastid development. The GhYGL1d gene has a DYW domain in C-terminal and is highly express in leaves, localized to the chloroplast fractions. GhYGL1d share high amino acid-sequence homology with AtECB2. In atecb2 mutant, overexpression of GhYGL1d rescued the seedling lethal phenotype and restored the editing of accD and ndhF transcripts. Silencing of GhYGL1d led to the reduction of chlorophyll and phenotypically yellow-green leaves in cotton. Compared with wild type, GhYGL1d-silenced cotton showed significant deformations of thylakoid structures. Furthermore, the transcription levels of plastid-encoded polymerase (PEP) and nuclear-encoded polymerase (NEP) dependent genes were decreased in GhYGL1d-silenced cotton. Conclusions Our data indicate that GhYGL1d not only contributes to the editing of accD and ndhF genes, but also affects the expression of NEP- and PEP-dependent genes to regulate the development of thylakoids, and therefore regulates leaf variegation in cotton. |
topic |
Chloroplast PPR Leaf variegation Cotton |
url |
http://link.springer.com/article/10.1186/s12870-019-1945-1 |
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