Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells.
A crucial step in generating de novo immune responses is the polarization of naive cognate CD4+ T cells by pathogen-triggered dendritic cells (DC). In the human setting, standardized DC-dependent systems are lacking to study molecular events during the initiation of a naive CD4+ T cell response. We...
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doaj-097864381b9a409aa213b6c43a060f262020-11-25T02:29:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0198e10372510.1371/journal.pone.0103725Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells.Tammy OthMelanie C A SchnijderbergBirgit L M G Senden-GijsbersWilfred T V GermeraadGerard M J BosJoris VanderlochtA crucial step in generating de novo immune responses is the polarization of naive cognate CD4+ T cells by pathogen-triggered dendritic cells (DC). In the human setting, standardized DC-dependent systems are lacking to study molecular events during the initiation of a naive CD4+ T cell response. We developed a TCR-restricted assay to compare different pathogen-triggered human DC for their capacities to instruct functional differentiation of autologous, naive CD4+ T cells. We demonstrated that this methodology can be applied to compare differently matured DC in terms of kinetics, direction, and magnitude of the naive CD4+ T cell response. Furthermore, we showed the applicability of this assay to study the T cell polarizing capacity of low-frequency blood-derived DC populations directly isolated ex vivo. This methodology for addressing APC-dependent instruction of naive CD4+ T cells in a human autologous setting will provide researchers with a valuable tool to gain more insight into molecular mechanisms occurring in the early phase of T cell polarization. In addition, it may also allow the study of pharmacological agents on DC-dependent T cell polarization in the human system.http://europepmc.org/articles/PMC4140687?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tammy Oth Melanie C A Schnijderberg Birgit L M G Senden-Gijsbers Wilfred T V Germeraad Gerard M J Bos Joris Vanderlocht |
spellingShingle |
Tammy Oth Melanie C A Schnijderberg Birgit L M G Senden-Gijsbers Wilfred T V Germeraad Gerard M J Bos Joris Vanderlocht Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells. PLoS ONE |
author_facet |
Tammy Oth Melanie C A Schnijderberg Birgit L M G Senden-Gijsbers Wilfred T V Germeraad Gerard M J Bos Joris Vanderlocht |
author_sort |
Tammy Oth |
title |
Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells. |
title_short |
Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells. |
title_full |
Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells. |
title_fullStr |
Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells. |
title_full_unstemmed |
Monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive CD4+ T cells. |
title_sort |
monitoring the initiation and kinetics of human dendritic cell-induced polarization of autologous naive cd4+ t cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
A crucial step in generating de novo immune responses is the polarization of naive cognate CD4+ T cells by pathogen-triggered dendritic cells (DC). In the human setting, standardized DC-dependent systems are lacking to study molecular events during the initiation of a naive CD4+ T cell response. We developed a TCR-restricted assay to compare different pathogen-triggered human DC for their capacities to instruct functional differentiation of autologous, naive CD4+ T cells. We demonstrated that this methodology can be applied to compare differently matured DC in terms of kinetics, direction, and magnitude of the naive CD4+ T cell response. Furthermore, we showed the applicability of this assay to study the T cell polarizing capacity of low-frequency blood-derived DC populations directly isolated ex vivo. This methodology for addressing APC-dependent instruction of naive CD4+ T cells in a human autologous setting will provide researchers with a valuable tool to gain more insight into molecular mechanisms occurring in the early phase of T cell polarization. In addition, it may also allow the study of pharmacological agents on DC-dependent T cell polarization in the human system. |
url |
http://europepmc.org/articles/PMC4140687?pdf=render |
work_keys_str_mv |
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