Mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters
Force transduction at cell-cell adhesions regulates tissue development, maintenance and adaptation. We developed computational and experimental approaches to quantify, with both sub-cellular and multi-cellular resolution, the dynamics of force transmission in cell clusters. Applying this technology...
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2014-12-01
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Online Access: | https://elifesciences.org/articles/03282 |
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doaj-097bfd0cbe364b198581c8e2c8a3e3f02021-05-04T23:33:47ZengeLife Sciences Publications LtdeLife2050-084X2014-12-01310.7554/eLife.03282Mapping the dynamics of force transduction at cell–cell junctions of epithelial clustersMei Rosa Ng0Achim Besser1Joan S Brugge2Gaudenz Danuser3Department of Cell Biology, Harvard Medical School, Boston, United StatesDepartment of Cell Biology, Harvard Medical School, Boston, United StatesDepartment of Cell Biology, Harvard Medical School, Boston, United StatesDepartment of Cell Biology, Harvard Medical School, Boston, United StatesForce transduction at cell-cell adhesions regulates tissue development, maintenance and adaptation. We developed computational and experimental approaches to quantify, with both sub-cellular and multi-cellular resolution, the dynamics of force transmission in cell clusters. Applying this technology to spontaneously-forming adherent epithelial cell clusters, we found that basal force fluctuations were coupled to E-cadherin localization at the level of individual cell-cell junctions. At the multi-cellular scale, cell-cell force exchange depended on the cell position within a cluster, and was adaptive to reconfigurations due to cell divisions or positional rearrangements. Importantly, force transmission through a cell required coordinated modulation of cell-matrix adhesion and actomyosin contractility in the cell and its neighbors. These data provide insights into mechanisms that could control mechanical stress homeostasis in dynamic epithelial tissues, and highlight our methods as a resource for the study of mechanotransduction in cell-cell adhesions.https://elifesciences.org/articles/03282cell adhesioncell-matrix interactionmechanotransductioncell-cell interactionintercellular adhesion moleculemechanical force |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mei Rosa Ng Achim Besser Joan S Brugge Gaudenz Danuser |
spellingShingle |
Mei Rosa Ng Achim Besser Joan S Brugge Gaudenz Danuser Mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters eLife cell adhesion cell-matrix interaction mechanotransduction cell-cell interaction intercellular adhesion molecule mechanical force |
author_facet |
Mei Rosa Ng Achim Besser Joan S Brugge Gaudenz Danuser |
author_sort |
Mei Rosa Ng |
title |
Mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters |
title_short |
Mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters |
title_full |
Mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters |
title_fullStr |
Mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters |
title_full_unstemmed |
Mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters |
title_sort |
mapping the dynamics of force transduction at cell–cell junctions of epithelial clusters |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2014-12-01 |
description |
Force transduction at cell-cell adhesions regulates tissue development, maintenance and adaptation. We developed computational and experimental approaches to quantify, with both sub-cellular and multi-cellular resolution, the dynamics of force transmission in cell clusters. Applying this technology to spontaneously-forming adherent epithelial cell clusters, we found that basal force fluctuations were coupled to E-cadherin localization at the level of individual cell-cell junctions. At the multi-cellular scale, cell-cell force exchange depended on the cell position within a cluster, and was adaptive to reconfigurations due to cell divisions or positional rearrangements. Importantly, force transmission through a cell required coordinated modulation of cell-matrix adhesion and actomyosin contractility in the cell and its neighbors. These data provide insights into mechanisms that could control mechanical stress homeostasis in dynamic epithelial tissues, and highlight our methods as a resource for the study of mechanotransduction in cell-cell adhesions. |
topic |
cell adhesion cell-matrix interaction mechanotransduction cell-cell interaction intercellular adhesion molecule mechanical force |
url |
https://elifesciences.org/articles/03282 |
work_keys_str_mv |
AT meirosang mappingthedynamicsofforcetransductionatcellcelljunctionsofepithelialclusters AT achimbesser mappingthedynamicsofforcetransductionatcellcelljunctionsofepithelialclusters AT joansbrugge mappingthedynamicsofforcetransductionatcellcelljunctionsofepithelialclusters AT gaudenzdanuser mappingthedynamicsofforcetransductionatcellcelljunctionsofepithelialclusters |
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1721476929925677056 |