Molecular epidemiology of nasal isolates of methicillin-resistant Staphylococcus aureus from Jordan
Summary: Asymptomatic carriage of methicillin-resistant Staphylococcus aureus (MRSA) can predispose the host to a wide range of infections. To inform public health strategies, this study sought to determine the prevalence and the phenotypic and genotypic characteristics of MRSA from nasal swabs of h...
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doaj-099c90fb13a8464ba4e308d086dd61e52020-11-25T02:41:14ZengElsevierJournal of Infection and Public Health1876-03412015-01-01819097Molecular epidemiology of nasal isolates of methicillin-resistant Staphylococcus aureus from JordanAmin A. Aqel0Hamed M. Alzoubi1Anna Vickers2Bruno Pichon3Angela M. Kearns4Microbiology and Immunology Department, Faculty of Medicine, Mu’tah University, Alkarak 61710, Jordan; Corresponding author at: Mu’tah University, Faculty of Medicine, Microbiology Department, P.O. Box 7, Mu’tah 61710, Alkarak, Jordan. Tel.: +962 3 238 6287x3208; fax: +962 3 239 7180.Microbiology and Immunology Department, Faculty of Medicine, Mu’tah University, Alkarak 61710, JordanAntimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UKAntimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UKAntimicrobial Resistance and Healthcare Associated Infections Reference Unit, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UKSummary: Asymptomatic carriage of methicillin-resistant Staphylococcus aureus (MRSA) can predispose the host to a wide range of infections. To inform public health strategies, this study sought to determine the prevalence and the phenotypic and genotypic characteristics of MRSA from nasal swabs of health care workers (HCWs) and other healthy individuals in Jordan. Overall, 716 nasal swabs were collected from 297 HCWs, 141 adults and 278 children in the community. MRSA was recovered from 56 (7.8%) nasal swabs, which represented carriage rates of 10.1%, 4.3% and 7.2% among HCWs, adults and children, respectively. The MRSA isolates were resistant to oxacillin (100%), erythromycin (42.8%), tetracycline (37.5%), clindamycin (5.3%), fucidin (5.3%), and ciprofloxacin (3.5%). A total of 17 different spa types belonging to eight different clonal complexes (CCs) were identified. All isolates were mecA positive, and mecC-MRSA was not detected. Analysis of the staphylococcal cassette chromosome mec (SCCmec) elements revealed that the majority (54; 96.4%) of the samples harbored the smaller type IV and V elements (the most common were SCCmec IVa or IVc, and there were two each of the IVg and V elements), and two were nontypable. The genes for Panton-Valentine leukocidin (luk-PV) were detected in 5.4% of the study isolates. A tst-positive, CC22-MRSA-SCCmecIVa clone (spa type t223) was identified as the dominant MRSA lineage among the nasal carriage isolates from both HCWs and other individuals (adults and children) in the community. These findings provide important information for public health personnel for the formulation of effective infection prevention and control strategies. Studies to further our understanding of the distribution, pathogenicity, transmissibility and fitness of this lineage would be prudent. Keywords: MRSA, Healthcare workers, Healthy individuals, SCCmecIVahttp://www.sciencedirect.com/science/article/pii/S187603411400080X |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amin A. Aqel Hamed M. Alzoubi Anna Vickers Bruno Pichon Angela M. Kearns |
spellingShingle |
Amin A. Aqel Hamed M. Alzoubi Anna Vickers Bruno Pichon Angela M. Kearns Molecular epidemiology of nasal isolates of methicillin-resistant Staphylococcus aureus from Jordan Journal of Infection and Public Health |
author_facet |
Amin A. Aqel Hamed M. Alzoubi Anna Vickers Bruno Pichon Angela M. Kearns |
author_sort |
Amin A. Aqel |
title |
Molecular epidemiology of nasal isolates of methicillin-resistant Staphylococcus aureus from Jordan |
title_short |
Molecular epidemiology of nasal isolates of methicillin-resistant Staphylococcus aureus from Jordan |
title_full |
Molecular epidemiology of nasal isolates of methicillin-resistant Staphylococcus aureus from Jordan |
title_fullStr |
Molecular epidemiology of nasal isolates of methicillin-resistant Staphylococcus aureus from Jordan |
title_full_unstemmed |
Molecular epidemiology of nasal isolates of methicillin-resistant Staphylococcus aureus from Jordan |
title_sort |
molecular epidemiology of nasal isolates of methicillin-resistant staphylococcus aureus from jordan |
publisher |
Elsevier |
series |
Journal of Infection and Public Health |
issn |
1876-0341 |
publishDate |
2015-01-01 |
description |
Summary: Asymptomatic carriage of methicillin-resistant Staphylococcus aureus (MRSA) can predispose the host to a wide range of infections. To inform public health strategies, this study sought to determine the prevalence and the phenotypic and genotypic characteristics of MRSA from nasal swabs of health care workers (HCWs) and other healthy individuals in Jordan. Overall, 716 nasal swabs were collected from 297 HCWs, 141 adults and 278 children in the community. MRSA was recovered from 56 (7.8%) nasal swabs, which represented carriage rates of 10.1%, 4.3% and 7.2% among HCWs, adults and children, respectively. The MRSA isolates were resistant to oxacillin (100%), erythromycin (42.8%), tetracycline (37.5%), clindamycin (5.3%), fucidin (5.3%), and ciprofloxacin (3.5%). A total of 17 different spa types belonging to eight different clonal complexes (CCs) were identified. All isolates were mecA positive, and mecC-MRSA was not detected. Analysis of the staphylococcal cassette chromosome mec (SCCmec) elements revealed that the majority (54; 96.4%) of the samples harbored the smaller type IV and V elements (the most common were SCCmec IVa or IVc, and there were two each of the IVg and V elements), and two were nontypable. The genes for Panton-Valentine leukocidin (luk-PV) were detected in 5.4% of the study isolates. A tst-positive, CC22-MRSA-SCCmecIVa clone (spa type t223) was identified as the dominant MRSA lineage among the nasal carriage isolates from both HCWs and other individuals (adults and children) in the community. These findings provide important information for public health personnel for the formulation of effective infection prevention and control strategies. Studies to further our understanding of the distribution, pathogenicity, transmissibility and fitness of this lineage would be prudent. Keywords: MRSA, Healthcare workers, Healthy individuals, SCCmecIVa |
url |
http://www.sciencedirect.com/science/article/pii/S187603411400080X |
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