New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints.
Culturing is an indispensable technique in microbiological research, and culturing with selective media has played a crucial role in the detection of pathogenic microorganisms and the isolation of commercially useful microorganisms from environmental samples. Although numerous selective media have b...
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2011-01-01
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doaj-099d8f29f21f4850b8e72c1e1288aa022020-11-25T02:57:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1651210.1371/journal.pone.0016512New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints.Takeshi KawanishiTakuya ShiraishiYukari OkanoKyoko SugawaraMasayoshi HashimotoKensaku MaejimaKen KomatsuShigeyuki KakizawaYasuyuki YamajiHiroshi HamamotoKenro OshimaShigetou NambaCulturing is an indispensable technique in microbiological research, and culturing with selective media has played a crucial role in the detection of pathogenic microorganisms and the isolation of commercially useful microorganisms from environmental samples. Although numerous selective media have been developed in empirical studies, unintended microorganisms often grow on such media probably due to the enormous numbers of microorganisms in the environment. Here, we present a novel strategy for designing highly selective media based on two selective agents, a carbon source and antimicrobials. We named our strategy SMART for highly Selective Medium-design Algorithm Restricted by Two constraints. To test whether the SMART method is applicable to a wide range of microorganisms, we developed selective media for Burkholderia glumae, Acidovorax avenae, Pectobacterium carotovorum, Ralstonia solanacearum, and Xanthomonas campestris. The series of media developed by SMART specifically allowed growth of the targeted bacteria. Because these selective media exhibited high specificity for growth of the target bacteria compared to established selective media, we applied three notable detection technologies: paper-based, flow cytometry-based, and color change-based detection systems for target bacteria species. SMART facilitates not only the development of novel techniques for detecting specific bacteria, but also our understanding of the ecology and epidemiology of the targeted bacteria.http://europepmc.org/articles/PMC3029383?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takeshi Kawanishi Takuya Shiraishi Yukari Okano Kyoko Sugawara Masayoshi Hashimoto Kensaku Maejima Ken Komatsu Shigeyuki Kakizawa Yasuyuki Yamaji Hiroshi Hamamoto Kenro Oshima Shigetou Namba |
spellingShingle |
Takeshi Kawanishi Takuya Shiraishi Yukari Okano Kyoko Sugawara Masayoshi Hashimoto Kensaku Maejima Ken Komatsu Shigeyuki Kakizawa Yasuyuki Yamaji Hiroshi Hamamoto Kenro Oshima Shigetou Namba New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints. PLoS ONE |
author_facet |
Takeshi Kawanishi Takuya Shiraishi Yukari Okano Kyoko Sugawara Masayoshi Hashimoto Kensaku Maejima Ken Komatsu Shigeyuki Kakizawa Yasuyuki Yamaji Hiroshi Hamamoto Kenro Oshima Shigetou Namba |
author_sort |
Takeshi Kawanishi |
title |
New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints. |
title_short |
New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints. |
title_full |
New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints. |
title_fullStr |
New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints. |
title_full_unstemmed |
New detection systems of bacteria using highly selective media designed by SMART: selective medium-design algorithm restricted by two constraints. |
title_sort |
new detection systems of bacteria using highly selective media designed by smart: selective medium-design algorithm restricted by two constraints. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Culturing is an indispensable technique in microbiological research, and culturing with selective media has played a crucial role in the detection of pathogenic microorganisms and the isolation of commercially useful microorganisms from environmental samples. Although numerous selective media have been developed in empirical studies, unintended microorganisms often grow on such media probably due to the enormous numbers of microorganisms in the environment. Here, we present a novel strategy for designing highly selective media based on two selective agents, a carbon source and antimicrobials. We named our strategy SMART for highly Selective Medium-design Algorithm Restricted by Two constraints. To test whether the SMART method is applicable to a wide range of microorganisms, we developed selective media for Burkholderia glumae, Acidovorax avenae, Pectobacterium carotovorum, Ralstonia solanacearum, and Xanthomonas campestris. The series of media developed by SMART specifically allowed growth of the targeted bacteria. Because these selective media exhibited high specificity for growth of the target bacteria compared to established selective media, we applied three notable detection technologies: paper-based, flow cytometry-based, and color change-based detection systems for target bacteria species. SMART facilitates not only the development of novel techniques for detecting specific bacteria, but also our understanding of the ecology and epidemiology of the targeted bacteria. |
url |
http://europepmc.org/articles/PMC3029383?pdf=render |
work_keys_str_mv |
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