Optimization of cyclosporin A production by Beauveria nivea in continuous fed-batch fermentation

• Main Authors: Dong Huijun, Jiang Junyun, Yan Tongshun, Zhao Junjie
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2011-01-01
• Series: Archives of Biological Sciences
• Subjects: cyclosporin A; fed-batch fermentation; Beauveria nivea
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2011/0354-46641103907D.pdf

To develop the effective control method for fed-batch culture of cyclosporin A production, we chose fructose, L-valine and (NH4)2HPO4 as feeding nutrients and compared their productivities in relation to different concentrations. The feeding rate of three kinds of feeding materials was controlled to maintain the suitable residual concentration. The fed-batch fermentation results indicated that the optimal concentrations of fructose, L-valine and (NH4)2HPO4 were about 20 g/L, 0.5 g/L and 0.6 g/L for cyclosporin A production, respectively. The cultivation of Beauveria nivea could produce cyclosporin A up to 6.2 g/L for 240 hrs through a continuous feeding-rate-controlled-batch process under the optimal feeding conditions.