A pilot study on ex vivo expanded autologous adipose‐derived stem cells of improving fat retention in localized scleroderma patients
Abstract In patients with localized scleroderma (LoS), facial deformity induced by subcutaneous atrophy greatly reduces life quality. Autologous fat grafting (AFG) is used for volume restoration but with low‐fat retention due to various reasons. Adipose‐derived stem cells (ADSCs) have shown potentia...
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Online Access: | https://doi.org/10.1002/sctm.20-0419 |
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doaj-09b650bd41d54151bc3b298794b4651e2021-07-16T17:56:04ZengWileyStem Cells Translational Medicine2157-65642157-65802021-08-011081148115610.1002/sctm.20-0419A pilot study on ex vivo expanded autologous adipose‐derived stem cells of improving fat retention in localized scleroderma patientsChenyu Wang0Xiao Long1Loubin Si2Bo Chen3Yiwei Zhang4Tianyu Sun5Xiuqin Zhang6Robert Chunhua Zhao7Xiaojun Wang8Department of Plastic Surgery, Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing People's Republic of ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing People's Republic of ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing People's Republic of ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing People's Republic of ChinaDepartment of Radiology, Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing People's Republic of ChinaRadcliffe Department of Medicine University of Oxford Oxford UKJimo Traditional Chinese Medicine Hospital Qingdao People's Republic of ChinaInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College and Chinese Academy of Medical Sciences Beijing People's Republic of ChinaDepartment of Plastic Surgery, Peking Union Medical College Hospital Peking Union Medical College and Chinese Academy of Medical Sciences Beijing People's Republic of ChinaAbstract In patients with localized scleroderma (LoS), facial deformity induced by subcutaneous atrophy greatly reduces life quality. Autologous fat grafting (AFG) is used for volume restoration but with low‐fat retention due to various reasons. Adipose‐derived stem cells (ADSCs) have shown potential effects in improving fat retention. We aimed to compare the feasibility and efficacy of improving fat retention in LoS patients among the ADSCs‐assisted, the stromal vascular fraction (SVF)‐assisted and conventional AFG methods. A pilot study with a 6‐month follow‐up among 18 LoS patients was conducted. Participants were randomly assigned into three AFG groups: conventional group, SVF‐assisted group, and ADSCs‐assisted group. The SVF‐assisted group received SVF‐assisted AFG at the SVF:fat ratio of 1:1. The ADSCs‐assisted group received the mixture of ADSCs‐enriched fat graft supplemented with 5 × 105 ADSCs/mL fat. Volume retention was measured by magnetic resonance imaging, and clinical photographs were taken for outcome evaluation. At sixth‐month follow‐up, the fat retention of ADSCs‐assisted group was 49.83 ± 3.61%, significantly higher than 31.75 ± 1.73% of SVF‐assisted group (P = .0004), and 21.86 ± 1.68% of the conventional group (P < .0001). A significant difference of the fat retention was also observed between the SVF‐assisted and conventional group (P = .0346). No severe adverse events occurred during the procedure and follow‐up. This pilot study suggests that ADSCs‐assisted AFG is a safe, feasible, and attractive alternative to conventional and SVF‐assisted AFG in the correction of facial atrophy of LoS patients. Future studies with large patient samples are needed for confirmation. (Chinese Clinical Trial Registry, ChiCTR1900025717).https://doi.org/10.1002/sctm.20-0419adipose‐derived stem cellsfat graftinglocalized sclerodermastromal vascular fraction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chenyu Wang Xiao Long Loubin Si Bo Chen Yiwei Zhang Tianyu Sun Xiuqin Zhang Robert Chunhua Zhao Xiaojun Wang |
spellingShingle |
Chenyu Wang Xiao Long Loubin Si Bo Chen Yiwei Zhang Tianyu Sun Xiuqin Zhang Robert Chunhua Zhao Xiaojun Wang A pilot study on ex vivo expanded autologous adipose‐derived stem cells of improving fat retention in localized scleroderma patients Stem Cells Translational Medicine adipose‐derived stem cells fat grafting localized scleroderma stromal vascular fraction |
author_facet |
Chenyu Wang Xiao Long Loubin Si Bo Chen Yiwei Zhang Tianyu Sun Xiuqin Zhang Robert Chunhua Zhao Xiaojun Wang |
author_sort |
Chenyu Wang |
title |
A pilot study on ex vivo expanded autologous adipose‐derived stem cells of improving fat retention in localized scleroderma patients |
title_short |
A pilot study on ex vivo expanded autologous adipose‐derived stem cells of improving fat retention in localized scleroderma patients |
title_full |
A pilot study on ex vivo expanded autologous adipose‐derived stem cells of improving fat retention in localized scleroderma patients |
title_fullStr |
A pilot study on ex vivo expanded autologous adipose‐derived stem cells of improving fat retention in localized scleroderma patients |
title_full_unstemmed |
A pilot study on ex vivo expanded autologous adipose‐derived stem cells of improving fat retention in localized scleroderma patients |
title_sort |
pilot study on ex vivo expanded autologous adipose‐derived stem cells of improving fat retention in localized scleroderma patients |
publisher |
Wiley |
series |
Stem Cells Translational Medicine |
issn |
2157-6564 2157-6580 |
publishDate |
2021-08-01 |
description |
Abstract In patients with localized scleroderma (LoS), facial deformity induced by subcutaneous atrophy greatly reduces life quality. Autologous fat grafting (AFG) is used for volume restoration but with low‐fat retention due to various reasons. Adipose‐derived stem cells (ADSCs) have shown potential effects in improving fat retention. We aimed to compare the feasibility and efficacy of improving fat retention in LoS patients among the ADSCs‐assisted, the stromal vascular fraction (SVF)‐assisted and conventional AFG methods. A pilot study with a 6‐month follow‐up among 18 LoS patients was conducted. Participants were randomly assigned into three AFG groups: conventional group, SVF‐assisted group, and ADSCs‐assisted group. The SVF‐assisted group received SVF‐assisted AFG at the SVF:fat ratio of 1:1. The ADSCs‐assisted group received the mixture of ADSCs‐enriched fat graft supplemented with 5 × 105 ADSCs/mL fat. Volume retention was measured by magnetic resonance imaging, and clinical photographs were taken for outcome evaluation. At sixth‐month follow‐up, the fat retention of ADSCs‐assisted group was 49.83 ± 3.61%, significantly higher than 31.75 ± 1.73% of SVF‐assisted group (P = .0004), and 21.86 ± 1.68% of the conventional group (P < .0001). A significant difference of the fat retention was also observed between the SVF‐assisted and conventional group (P = .0346). No severe adverse events occurred during the procedure and follow‐up. This pilot study suggests that ADSCs‐assisted AFG is a safe, feasible, and attractive alternative to conventional and SVF‐assisted AFG in the correction of facial atrophy of LoS patients. Future studies with large patient samples are needed for confirmation. (Chinese Clinical Trial Registry, ChiCTR1900025717). |
topic |
adipose‐derived stem cells fat grafting localized scleroderma stromal vascular fraction |
url |
https://doi.org/10.1002/sctm.20-0419 |
work_keys_str_mv |
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