Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate
Abstract Background In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel Rhodosporidium toruloides mutant strain, RM18, was isolated through atmospheric and room-temperature plasma mutagenesis and continuous domestication in te...
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doaj-09bda4afe23c4dd399856487113eb8fb2020-11-25T02:29:59ZengBMCBiotechnology for Biofuels1754-68342020-04-0113111210.1186/s13068-020-01712-0Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysateFeng Qi0Peijie Shen1Rongfei Hu2Ting Xue3Xianzhang Jiang4Lina Qin5Youqiang Chen6Jianzhong Huang7Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal UniversityEngineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal UniversityEngineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal UniversityMedicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, College of Life Sciences, Fujian Normal UniversityEngineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal UniversityEngineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal UniversityMedicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, College of Life Sciences, Fujian Normal UniversityEngineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal UniversityAbstract Background In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel Rhodosporidium toruloides mutant strain, RM18, was isolated through atmospheric and room-temperature plasma mutagenesis and continuous domestication in tea waste hydrolysate from R. toruloides ACCC20341. Results RM18 produced a larger biomass and more carotenoids and α-linolenic acid compared with the control strain cultured in tea waste hydrolysate. The highest yields of torularhodin (481.92 μg/g DCW) and torulene (501 μg/g DCW) from RM18 cultured in tea waste hydrolysate were 12.86- and 1.5-fold higher, respectively, than that of the control strain. In addition, α-linolenic acid production from RM18 in TWH accounted for 5.5% of total lipids, which was 1.58 times more than that of the control strain. Transcriptomic profiling indicated that enhanced central metabolism and terpene biosynthesis led to improved carotenoids production, whereas aromatic amino acid synthesis and DNA damage checkpoint and sensing were probably relevant to tea waste hydrolysate tolerance. Conclusion Tea waste is suitable for the hydrolysis of microbial cell culture mediums. The R. toruloides mutant RM18 showed considerable carotenoids and lipid production cultured in tea waste hydrolysate, which makes it viable for industrial applications.http://link.springer.com/article/10.1186/s13068-020-01712-0R. toruloidesTea waste hydrolysateTorularhodinToruleneLipid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feng Qi Peijie Shen Rongfei Hu Ting Xue Xianzhang Jiang Lina Qin Youqiang Chen Jianzhong Huang |
spellingShingle |
Feng Qi Peijie Shen Rongfei Hu Ting Xue Xianzhang Jiang Lina Qin Youqiang Chen Jianzhong Huang Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate Biotechnology for Biofuels R. toruloides Tea waste hydrolysate Torularhodin Torulene Lipid |
author_facet |
Feng Qi Peijie Shen Rongfei Hu Ting Xue Xianzhang Jiang Lina Qin Youqiang Chen Jianzhong Huang |
author_sort |
Feng Qi |
title |
Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate |
title_short |
Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate |
title_full |
Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate |
title_fullStr |
Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate |
title_full_unstemmed |
Carotenoids and lipid production from Rhodosporidium toruloides cultured in tea waste hydrolysate |
title_sort |
carotenoids and lipid production from rhodosporidium toruloides cultured in tea waste hydrolysate |
publisher |
BMC |
series |
Biotechnology for Biofuels |
issn |
1754-6834 |
publishDate |
2020-04-01 |
description |
Abstract Background In this study, renewable tea waste hydrolysate was used as a sole carbon source for carotenoids and lipid production. A novel Rhodosporidium toruloides mutant strain, RM18, was isolated through atmospheric and room-temperature plasma mutagenesis and continuous domestication in tea waste hydrolysate from R. toruloides ACCC20341. Results RM18 produced a larger biomass and more carotenoids and α-linolenic acid compared with the control strain cultured in tea waste hydrolysate. The highest yields of torularhodin (481.92 μg/g DCW) and torulene (501 μg/g DCW) from RM18 cultured in tea waste hydrolysate were 12.86- and 1.5-fold higher, respectively, than that of the control strain. In addition, α-linolenic acid production from RM18 in TWH accounted for 5.5% of total lipids, which was 1.58 times more than that of the control strain. Transcriptomic profiling indicated that enhanced central metabolism and terpene biosynthesis led to improved carotenoids production, whereas aromatic amino acid synthesis and DNA damage checkpoint and sensing were probably relevant to tea waste hydrolysate tolerance. Conclusion Tea waste is suitable for the hydrolysis of microbial cell culture mediums. The R. toruloides mutant RM18 showed considerable carotenoids and lipid production cultured in tea waste hydrolysate, which makes it viable for industrial applications. |
topic |
R. toruloides Tea waste hydrolysate Torularhodin Torulene Lipid |
url |
http://link.springer.com/article/10.1186/s13068-020-01712-0 |
work_keys_str_mv |
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