Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activity
Background: There is an increasing need to find natural bioactive compounds for pharmaceutical applications, because they have less harmful side effects compared to their chemical alternatives. Microalgae (MA) have been identified as a promising source for these bioactive compounds, and this work ai...
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doaj-09d31f72086e4925bbe8ebd7e8a386da2021-02-02T03:12:42ZengElsevierBiomedical Journal2319-41702017-12-0140633934610.1016/j.bj.2017.10.003Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activityAsma Jabeen0Brandon Reeder1Soleiman Hisaindee2Salman Ashraf3Naeema Al Darmaki4Sinan Battah5Sulaiman Al-Zuhair6School of Biological Sciences, University of Essex, Colchester, UKSchool of Biological Sciences, University of Essex, Colchester, UKChemistry Department, UAE University, Al-Ain, United Arab EmiratesChemistry Department, UAE University, Al-Ain, United Arab EmiratesChemical and Petroleum Engineering Department, UAE University, Al-Ain, United Arab EmiratesSchool of Biological Sciences, University of Essex, Colchester, UKChemical and Petroleum Engineering Department, UAE University, Al-Ain, United Arab EmiratesBackground: There is an increasing need to find natural bioactive compounds for pharmaceutical applications, because they have less harmful side effects compared to their chemical alternatives. Microalgae (MA) have been identified as a promising source for these bioactive compounds, and this work aimed to evaluate the anti-proliferative effects of semi-purified protein extracted from MA against several tumor cell lines. Methods: Tested samples comprised MA cell extracts treated with cellulase and lysozyme, prior to extraction. The effect of dialysis, required to remove unnecessary small molecules, was also tested. The anti-cancer efficacies of the dialyzed and undialyzed extracts were determined by measuring cell viability after treating four human cancer cell lines, specifically A549 (human lung carcinoma), MCF-7 (human breast adenocarcinoma), MDA MB-435 (human melanoma), and LNCap (human prostate cancer cells derived from a metastatic site in the lymph node). This was compared to the effects of the agents on the human BPH-1 cell line (benign human prostate epithelial cells). The t-test was used to statistically analyze the results and determine the significance. Results: Against LNCap and A549 cells, the performance of cellulase-treated extracts was better (with p-values < 0.05, as compared to the control) than that of lysozyme-treated preparations (with p-values mainly > 0.05, as compared to the control); however, they had similar effects against the other two tumor cell lines (with p-values mainly < 0.05, as compared to the control). Moreover, based on their effect on BPH-1 cells, extracts from lysozyme-treated MA cells were determined to be safer against the benign prostate hyperplasia cells, BPH-1 (with p-values mainly > 0.05, as compared to the control). After dialysis, the performance of MA extracts from lysozyme-treated cells was enhanced significantly (with p-values dropping to < 0.05, as compared to the control). Conclusions: The results of this work provide important information and could provide the foundation for further research to incorporate MA constituents into pharmaceutical anti-cancer therapeutic formulations.http://www.sciencedirect.com/science/article/pii/S2319417017300276ProliferationAnti-cancer agentsMicroalgaeProteinsEnzymatic extraction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Asma Jabeen Brandon Reeder Soleiman Hisaindee Salman Ashraf Naeema Al Darmaki Sinan Battah Sulaiman Al-Zuhair |
spellingShingle |
Asma Jabeen Brandon Reeder Soleiman Hisaindee Salman Ashraf Naeema Al Darmaki Sinan Battah Sulaiman Al-Zuhair Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activity Biomedical Journal Proliferation Anti-cancer agents Microalgae Proteins Enzymatic extraction |
author_facet |
Asma Jabeen Brandon Reeder Soleiman Hisaindee Salman Ashraf Naeema Al Darmaki Sinan Battah Sulaiman Al-Zuhair |
author_sort |
Asma Jabeen |
title |
Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activity |
title_short |
Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activity |
title_full |
Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activity |
title_fullStr |
Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activity |
title_full_unstemmed |
Effect of Enzymatic pre-treatment of microalgae extracts on their anti-tumor activity |
title_sort |
effect of enzymatic pre-treatment of microalgae extracts on their anti-tumor activity |
publisher |
Elsevier |
series |
Biomedical Journal |
issn |
2319-4170 |
publishDate |
2017-12-01 |
description |
Background: There is an increasing need to find natural bioactive compounds for pharmaceutical applications, because they have less harmful side effects compared to their chemical alternatives. Microalgae (MA) have been identified as a promising source for these bioactive compounds, and this work aimed to evaluate the anti-proliferative effects of semi-purified protein extracted from MA against several tumor cell lines.
Methods: Tested samples comprised MA cell extracts treated with cellulase and lysozyme, prior to extraction. The effect of dialysis, required to remove unnecessary small molecules, was also tested. The anti-cancer efficacies of the dialyzed and undialyzed extracts were determined by measuring cell viability after treating four human cancer cell lines, specifically A549 (human lung carcinoma), MCF-7 (human breast adenocarcinoma), MDA MB-435 (human melanoma), and LNCap (human prostate cancer cells derived from a metastatic site in the lymph node). This was compared to the effects of the agents on the human BPH-1 cell line (benign human prostate epithelial cells). The t-test was used to statistically analyze the results and determine the significance.
Results: Against LNCap and A549 cells, the performance of cellulase-treated extracts was better (with p-values < 0.05, as compared to the control) than that of lysozyme-treated preparations (with p-values mainly > 0.05, as compared to the control); however, they had similar effects against the other two tumor cell lines (with p-values mainly < 0.05, as compared to the control). Moreover, based on their effect on BPH-1 cells, extracts from lysozyme-treated MA cells were determined to be safer against the benign prostate hyperplasia cells, BPH-1 (with p-values mainly > 0.05, as compared to the control). After dialysis, the performance of MA extracts from lysozyme-treated cells was enhanced significantly (with p-values dropping to < 0.05, as compared to the control).
Conclusions: The results of this work provide important information and could provide the foundation for further research to incorporate MA constituents into pharmaceutical anti-cancer therapeutic formulations. |
topic |
Proliferation Anti-cancer agents Microalgae Proteins Enzymatic extraction |
url |
http://www.sciencedirect.com/science/article/pii/S2319417017300276 |
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