Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines
Abstract Background Phospholipases A 2 (PLA 2 s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA 2isolated...
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doaj-09d3c7bbd0b1489ba1b6258114dc34b52020-11-25T01:48:52ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992015-12-0121010.1186/s40409-015-0044-5S1678-91992015000100348Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell linesCássio Prinholato da SilvaTássia R. CostaRaquel M. Alves PaivaAdélia C. O. CintraDanilo L. MenaldoLusânia M. Greggi AntunesSuely V. SampaioAbstract Background Phospholipases A 2 (PLA 2 s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA 2isolated from Bothrops jararacussu venom, by evaluating in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human (HL-60 and HepG2) and murine (PC-12 and B16F10) tumor cell lines. Methods The cytotoxic effects of BthTX-I were evaluated on the tumor cell lines HL-60 (promyelocytic leukemia), HepG2 (human hepatocellular carcinoma), PC-12 (murine pheochromocytoma) and B16F10 (murine melanoma) using the MTT method. Flow cytometry technique was used for the analysis of cell cycle alterations and death mechanisms (apoptosis and/or necrosis) induced in tumor cells after treatment with BthTX-I. Results It was observed that BthTX-I was cytotoxic to all evaluated tumor cell lines, reducing their viability in 40 to 50 %. The myotoxin showed modulating effects on the cell cycle of PC-12 and B16F10 cells, promoting delay in the G0/G1 phase. Additionally, flow cytometry analysis indicated cell death mainly by apoptosis. B16F10 was more susceptible to the effects of BthTX-I, with ~40 % of the cells analyzed in apoptosis, followed by HepG2 (~35 %), PC-12 (~25 %) and HL-60 (~4 %). Conclusions These results suggest that BthTX-I presents antitumor properties that may be useful for developing new therapeutic strategies against cancer.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100348&lng=en&tlng=enBothrops jararacussuBthTX-IAntitumor potentialApoptosisCell cycle alterations |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cássio Prinholato da Silva Tássia R. Costa Raquel M. Alves Paiva Adélia C. O. Cintra Danilo L. Menaldo Lusânia M. Greggi Antunes Suely V. Sampaio |
spellingShingle |
Cássio Prinholato da Silva Tássia R. Costa Raquel M. Alves Paiva Adélia C. O. Cintra Danilo L. Menaldo Lusânia M. Greggi Antunes Suely V. Sampaio Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines Journal of Venomous Animals and Toxins including Tropical Diseases Bothrops jararacussu BthTX-I Antitumor potential Apoptosis Cell cycle alterations |
author_facet |
Cássio Prinholato da Silva Tássia R. Costa Raquel M. Alves Paiva Adélia C. O. Cintra Danilo L. Menaldo Lusânia M. Greggi Antunes Suely V. Sampaio |
author_sort |
Cássio Prinholato da Silva |
title |
Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines |
title_short |
Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines |
title_full |
Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines |
title_fullStr |
Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines |
title_full_unstemmed |
Antitumor potential of the myotoxin BthTX-I from Bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines |
title_sort |
antitumor potential of the myotoxin bthtx-i from bothrops jararacussu snake venom: evaluation of cell cycle alterations and death mechanisms induced in tumor cell lines |
publisher |
SciELO |
series |
Journal of Venomous Animals and Toxins including Tropical Diseases |
issn |
1678-9199 |
publishDate |
2015-12-01 |
description |
Abstract Background Phospholipases A 2 (PLA 2 s) are abundant components of snake venoms that have been extensively studied due to their pharmacological and pathophysiological effects on living organisms. This study aimed to assess the antitumor potential of BthTX-I, a basic myotoxic PLA 2isolated from Bothrops jararacussu venom, by evaluating in vitro processes of cytotoxicity, modulation of the cell cycle and induction of apoptosis in human (HL-60 and HepG2) and murine (PC-12 and B16F10) tumor cell lines. Methods The cytotoxic effects of BthTX-I were evaluated on the tumor cell lines HL-60 (promyelocytic leukemia), HepG2 (human hepatocellular carcinoma), PC-12 (murine pheochromocytoma) and B16F10 (murine melanoma) using the MTT method. Flow cytometry technique was used for the analysis of cell cycle alterations and death mechanisms (apoptosis and/or necrosis) induced in tumor cells after treatment with BthTX-I. Results It was observed that BthTX-I was cytotoxic to all evaluated tumor cell lines, reducing their viability in 40 to 50 %. The myotoxin showed modulating effects on the cell cycle of PC-12 and B16F10 cells, promoting delay in the G0/G1 phase. Additionally, flow cytometry analysis indicated cell death mainly by apoptosis. B16F10 was more susceptible to the effects of BthTX-I, with ~40 % of the cells analyzed in apoptosis, followed by HepG2 (~35 %), PC-12 (~25 %) and HL-60 (~4 %). Conclusions These results suggest that BthTX-I presents antitumor properties that may be useful for developing new therapeutic strategies against cancer. |
topic |
Bothrops jararacussu BthTX-I Antitumor potential Apoptosis Cell cycle alterations |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100348&lng=en&tlng=en |
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