LncRNA SNHG3 sponges miR‐577 to up‐regulate SMURF1 expression in prostate cancer
Abstract Prostate cancer remains one of the most prevalent cancers and the main causes of cancer‐related deaths in males. Various articles introduced that long noncoding RNAs (lncRNAs) are found in vital functions in the development and progression of cancers. Although SNHG3 (small nucleolar RNA hos...
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doaj-09d6cf7cd7ac486b97999f0def79ed2a2020-11-25T03:03:51ZengWileyCancer Medicine2045-76342020-06-019113852386210.1002/cam4.2992LncRNA SNHG3 sponges miR‐577 to up‐regulate SMURF1 expression in prostate cancerTeng Li0Yi Xing1Fan Yang2Yangyang Sun3Shaojin Zhang4Qingwei Wang5Weixing Zhang6Department of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Opthalmology The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaDepartment of Urology The First Affiliated Hospital of Zhengzhou University Zhengzhou ChinaAbstract Prostate cancer remains one of the most prevalent cancers and the main causes of cancer‐related deaths in males. Various articles introduced that long noncoding RNAs (lncRNAs) are found in vital functions in the development and progression of cancers. Although SNHG3 (small nucleolar RNA host gene 3) has been investigated in many cancers, now researches on the role and mechanism of SNHG3 in prostate cancer are lacked. In this work, SNHG3 exerted high expression in prostate cancer cell lines. Suppression of SNHG3 inhibited cell proliferation, migration, EMT (epithelial‐mesenchymal transition) process and promoted cell apoptosis. Additionally, it was found that SNHG3 could bind with miR‐577. Subsequently, SMURF1 (Smad ubiquitination regulatory factor 1) was identified as a downstream target of miR‐577 and had a negative correlation with miR‐577. SNHG3 was found to positively regulate SMURF1 expression. Furthermore, rescue assays demonstrated that co‐transfection of pcDNA3.1/SMURF1 reversed the effects of SNHG3 knockdown in cell proliferation, migration, EMT process and cell apoptosis. SNHG3 also promoted tumorigenesis in vivo. All the results above explained that SNHG3 accelerated prostate cancer progression by sponging miR‐577 to up‐regulate SMURF1 expression, suggesting that SNHG3 may act as a biomarker for prostate cancer patients.https://doi.org/10.1002/cam4.2992miR‐577prostate cancerSMURF1SNHG3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Teng Li Yi Xing Fan Yang Yangyang Sun Shaojin Zhang Qingwei Wang Weixing Zhang |
spellingShingle |
Teng Li Yi Xing Fan Yang Yangyang Sun Shaojin Zhang Qingwei Wang Weixing Zhang LncRNA SNHG3 sponges miR‐577 to up‐regulate SMURF1 expression in prostate cancer Cancer Medicine miR‐577 prostate cancer SMURF1 SNHG3 |
author_facet |
Teng Li Yi Xing Fan Yang Yangyang Sun Shaojin Zhang Qingwei Wang Weixing Zhang |
author_sort |
Teng Li |
title |
LncRNA SNHG3 sponges miR‐577 to up‐regulate SMURF1 expression in prostate cancer |
title_short |
LncRNA SNHG3 sponges miR‐577 to up‐regulate SMURF1 expression in prostate cancer |
title_full |
LncRNA SNHG3 sponges miR‐577 to up‐regulate SMURF1 expression in prostate cancer |
title_fullStr |
LncRNA SNHG3 sponges miR‐577 to up‐regulate SMURF1 expression in prostate cancer |
title_full_unstemmed |
LncRNA SNHG3 sponges miR‐577 to up‐regulate SMURF1 expression in prostate cancer |
title_sort |
lncrna snhg3 sponges mir‐577 to up‐regulate smurf1 expression in prostate cancer |
publisher |
Wiley |
series |
Cancer Medicine |
issn |
2045-7634 |
publishDate |
2020-06-01 |
description |
Abstract Prostate cancer remains one of the most prevalent cancers and the main causes of cancer‐related deaths in males. Various articles introduced that long noncoding RNAs (lncRNAs) are found in vital functions in the development and progression of cancers. Although SNHG3 (small nucleolar RNA host gene 3) has been investigated in many cancers, now researches on the role and mechanism of SNHG3 in prostate cancer are lacked. In this work, SNHG3 exerted high expression in prostate cancer cell lines. Suppression of SNHG3 inhibited cell proliferation, migration, EMT (epithelial‐mesenchymal transition) process and promoted cell apoptosis. Additionally, it was found that SNHG3 could bind with miR‐577. Subsequently, SMURF1 (Smad ubiquitination regulatory factor 1) was identified as a downstream target of miR‐577 and had a negative correlation with miR‐577. SNHG3 was found to positively regulate SMURF1 expression. Furthermore, rescue assays demonstrated that co‐transfection of pcDNA3.1/SMURF1 reversed the effects of SNHG3 knockdown in cell proliferation, migration, EMT process and cell apoptosis. SNHG3 also promoted tumorigenesis in vivo. All the results above explained that SNHG3 accelerated prostate cancer progression by sponging miR‐577 to up‐regulate SMURF1 expression, suggesting that SNHG3 may act as a biomarker for prostate cancer patients. |
topic |
miR‐577 prostate cancer SMURF1 SNHG3 |
url |
https://doi.org/10.1002/cam4.2992 |
work_keys_str_mv |
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