Genetic Architecture of Flooding Tolerance in the Dry Bean Middle-American Diversity Panel
Flooding is a devastating abiotic stress that endangers crop production in the twenty-first century. Because of the severe susceptibility of common bean (Phaseolus vulgaris L.) to flooding, an understanding of the genetic architecture and physiological responses of this crop will set the stage for f...
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doaj-09d8d91a4c0d4d52957e9d4e5d87aae22020-11-25T00:03:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-07-01810.3389/fpls.2017.01183276103Genetic Architecture of Flooding Tolerance in the Dry Bean Middle-American Diversity PanelAli Soltani0Samira MafiMoghaddam1Katelynn Walter2Daniel Restrepo-Montoya3Sujan Mamidi4Stephan Schroder5Rian Lee6Phillip E. McClean7Juan M. Osorno8Department of Plant Sciences, North Dakota State UniversityFargo, ND, United StatesDepartment of Plant Sciences, North Dakota State UniversityFargo, ND, United StatesDepartment of Plant Sciences, North Dakota State UniversityFargo, ND, United StatesDepartment of Plant Sciences, North Dakota State UniversityFargo, ND, United StatesGenome Sequencing Center, HudsonAlpha Institute for BiotechnologyHuntsville, AL, United StatesDepartment of Plant Sciences, North Dakota State UniversityFargo, ND, United StatesDepartment of Plant Sciences, North Dakota State UniversityFargo, ND, United StatesDepartment of Plant Sciences, North Dakota State UniversityFargo, ND, United StatesDepartment of Plant Sciences, North Dakota State UniversityFargo, ND, United StatesFlooding is a devastating abiotic stress that endangers crop production in the twenty-first century. Because of the severe susceptibility of common bean (Phaseolus vulgaris L.) to flooding, an understanding of the genetic architecture and physiological responses of this crop will set the stage for further improvement. However, challenging phenotyping methods hinder a large-scale genetic study of flooding tolerance in common bean and other economically important crops. A greenhouse phenotyping protocol was developed to evaluate the flooding conditions at early stages. The Middle-American diversity panel (n = 272) of common bean was developed to capture most of the diversity exits in North American germplasm. This panel was evaluated for seven traits under both flooded and non-flooded conditions at two early developmental stages. A subset of contrasting genotypes was further evaluated in the field to assess the relationship between greenhouse and field data under flooding condition. A genome-wide association study using ~150 K SNPs was performed to discover genomic regions associated with multiple physiological responses. The results indicate a significant strong correlation (r > 0.77) between greenhouse and field data, highlighting the reliability of greenhouse phenotyping method. Black and small red beans were the least affected by excess water at germination stage. At the seedling stage, pinto and great northern genotypes were the most tolerant. Root weight reduction due to flooding was greatest in pink and small red cultivars. Flooding reduced the chlorophyll content to the greatest extent in the navy bean cultivars compared with other market classes. Races of Durango/Jalisco and Mesoamerica were separated by both genotypic and phenotypic data indicating the potential effect of eco-geographical variations. Furthermore, several loci were identified that potentially represent the antagonistic pleiotropy. The GWAS analysis revealed peaks at Pv08/1.6 Mb and Pv02/41 Mb that are associated with root weight and germination rate, respectively. These regions are syntenic with two QTL reported in soybean (Glycine max L.) that contribute to flooding tolerance, suggesting a conserved evolutionary pathway involved in flooding tolerance for these related legumes.http://journal.frontiersin.org/article/10.3389/fpls.2017.01183/fullcommon beanfloodingabiotic stressanoxiawaterloggingGWAS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ali Soltani Samira MafiMoghaddam Katelynn Walter Daniel Restrepo-Montoya Sujan Mamidi Stephan Schroder Rian Lee Phillip E. McClean Juan M. Osorno |
spellingShingle |
Ali Soltani Samira MafiMoghaddam Katelynn Walter Daniel Restrepo-Montoya Sujan Mamidi Stephan Schroder Rian Lee Phillip E. McClean Juan M. Osorno Genetic Architecture of Flooding Tolerance in the Dry Bean Middle-American Diversity Panel Frontiers in Plant Science common bean flooding abiotic stress anoxia waterlogging GWAS |
author_facet |
Ali Soltani Samira MafiMoghaddam Katelynn Walter Daniel Restrepo-Montoya Sujan Mamidi Stephan Schroder Rian Lee Phillip E. McClean Juan M. Osorno |
author_sort |
Ali Soltani |
title |
Genetic Architecture of Flooding Tolerance in the Dry Bean Middle-American Diversity Panel |
title_short |
Genetic Architecture of Flooding Tolerance in the Dry Bean Middle-American Diversity Panel |
title_full |
Genetic Architecture of Flooding Tolerance in the Dry Bean Middle-American Diversity Panel |
title_fullStr |
Genetic Architecture of Flooding Tolerance in the Dry Bean Middle-American Diversity Panel |
title_full_unstemmed |
Genetic Architecture of Flooding Tolerance in the Dry Bean Middle-American Diversity Panel |
title_sort |
genetic architecture of flooding tolerance in the dry bean middle-american diversity panel |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2017-07-01 |
description |
Flooding is a devastating abiotic stress that endangers crop production in the twenty-first century. Because of the severe susceptibility of common bean (Phaseolus vulgaris L.) to flooding, an understanding of the genetic architecture and physiological responses of this crop will set the stage for further improvement. However, challenging phenotyping methods hinder a large-scale genetic study of flooding tolerance in common bean and other economically important crops. A greenhouse phenotyping protocol was developed to evaluate the flooding conditions at early stages. The Middle-American diversity panel (n = 272) of common bean was developed to capture most of the diversity exits in North American germplasm. This panel was evaluated for seven traits under both flooded and non-flooded conditions at two early developmental stages. A subset of contrasting genotypes was further evaluated in the field to assess the relationship between greenhouse and field data under flooding condition. A genome-wide association study using ~150 K SNPs was performed to discover genomic regions associated with multiple physiological responses. The results indicate a significant strong correlation (r > 0.77) between greenhouse and field data, highlighting the reliability of greenhouse phenotyping method. Black and small red beans were the least affected by excess water at germination stage. At the seedling stage, pinto and great northern genotypes were the most tolerant. Root weight reduction due to flooding was greatest in pink and small red cultivars. Flooding reduced the chlorophyll content to the greatest extent in the navy bean cultivars compared with other market classes. Races of Durango/Jalisco and Mesoamerica were separated by both genotypic and phenotypic data indicating the potential effect of eco-geographical variations. Furthermore, several loci were identified that potentially represent the antagonistic pleiotropy. The GWAS analysis revealed peaks at Pv08/1.6 Mb and Pv02/41 Mb that are associated with root weight and germination rate, respectively. These regions are syntenic with two QTL reported in soybean (Glycine max L.) that contribute to flooding tolerance, suggesting a conserved evolutionary pathway involved in flooding tolerance for these related legumes. |
topic |
common bean flooding abiotic stress anoxia waterlogging GWAS |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.01183/full |
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