KLONING OF GLUCOSE OXIDASE GENE IN PENICILLLIUM ADAMETZII LF F-2044.1

KLONING OF GLUCOSE OXIDASE GENE IN PENICILLLIUM ADAMETZII LF F-2044.1

Vector pNOM102-GOX was engineered to cloning gene gox in P. adametzii LF F-2044.1 and conditions were optimized for production of fungal protoplasts. Electroporation of P. adametzii LF F-2044.1 was conducted and transformants resistant to antibiotic geneticin were selected. Strains P. adametzii LF F...

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Bibliographic Details
Main Authors: Л. О. Жуковська, Р. В. Міхайлова, Т. В. Семашко, А. Г. Лобанок, Д. Г. Ярмолінский, Н. А. Картель
Format: Article
Language:English
Published: Odessa I. I. Mechnikov National University 2007-09-01
Series:Mìkrobìologìâ ì Bìotehnologìâ
Subjects:
penicillium adametzii
глюкозооксидаза
трансформація
ген gox
Online Access:http://mbt.onu.edu.ua/article/view/105029
Description
Summary:Vector pNOM102-GOX was engineered to cloning gene gox in P. adametzii LF F-2044.1 and conditions were optimized for production of fungal protoplasts. Electroporation of P. adametzii LF F-2044.1 was conducted and transformants resistant to antibiotic geneticin were selected. Strains P. adametzii LF F-2044.1.17 and P. adametzii LF F-2044.1.18 displayed increased levels of glucose oxidase synthesis - mycelium productivity rose 2 - 2.5 times. It was found that efficient preservation of vectors in the transformants requires maintenance on the media containing antibiotic geneticin.
ISSN:2076-0558
2307-4663

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