Studies on luciferin-luciferase ATP assay in plants (etiolated wheat germs, and bean leaves)

• Main Authors: A. Barbaro, S. Rożek
• Format: Article
• Language: English
• Published: Polish Botanical Society 2015-01-01
• Series: Acta Societatis Botanicorum Poloniae
• Online Access: https://pbsociety.org.pl/journals/index.php/asbp/article/view/5033

For ATP determination by the method of bioluminescence apparatus of home production was adapted from the equipment available in any isotope laboratory. The measurement error did not exceed 1.5 per cent. Methodical experiments concerned the choice of the extraction, fixation and storage methods of plant material for determination at the given moment of the amount of ATP in the tissues, unchanged by the analytical procedure. The highest ATP amounts were recovered by extraction with perchloric acid at high (25%) concentrations of the tissue in the homogenate. The best way of fixation of the material for later analyses was found to be freezing of ready extracts. Lyophilization and freezing of the plant material caused a several-fold decrease of the ATP level in the tissues. These results suggest the necessity of working in conditions of low temperature during the entire analytical procedure and strict observation of time limitation.