Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis
Although anti-angiogenic therapies (AATs) have some effects against multiple malignancies, they are limited by subsequent tumor vasculogenesis and progression. To investigate the mechanisms by which tumor vasculogenesis and progression following AATs, we transfected microRNA (miR)-9 into human umbil...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2019-12-01
|
Series: | Journal of Extracellular Vesicles |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/20013078.2019.1629865 |
id |
doaj-09fdca6db19f4c42928c7f3758a0d1e7 |
---|---|
record_format |
Article |
spelling |
doaj-09fdca6db19f4c42928c7f3758a0d1e72020-11-25T02:33:49ZengTaylor & Francis GroupJournal of Extracellular Vesicles2001-30782019-12-018110.1080/20013078.2019.16298651629865Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesisYe Zeng0Xinghong Yao1Xiaoheng Liu2Xueling He3Liang Li4Xiaojing Liu5Zhiping Yan6Jiang Wu7Bingmei M. Fu8Sichuan UniversitySichuan Cancer Hospital and InstituteSichuan UniversitySichuan UniversitySichuan UniversitySichuan UniversitySichuan UniversitySichuan UniversityThe City College of the City University of New YorkAlthough anti-angiogenic therapies (AATs) have some effects against multiple malignancies, they are limited by subsequent tumor vasculogenesis and progression. To investigate the mechanisms by which tumor vasculogenesis and progression following AATs, we transfected microRNA (miR)-9 into human umbilical vein endothelial cells (HUVECs) to mimic the tumor-associated endothelial cells in hepatocellular carcinoma and simulated the AATs in vitro and in vivo. We found that administration of the angiogenesis inhibitor vandetanib completely abolished miR-9-induced angiogenesis and promoted autophagy in HUVECs, but induced the release of vascular endothelial growth factor (VEGF)-enriched exosomes. These VEGF-enriched exosomes significantly promoted the formation of endothelial vessels and vasculogenic mimicry in hepatocellular carcinoma and its progression in mice. Anti-autophagic therapy is proposed to improve the efficacy of AATs. However, similar effects by AATs were observed with the application of anti-autophagy by 3-methyladenine. Our results revealed that tumor vasculogenesis and progression after AATs and anti-autophagic therapies were due to the cross-talk between endothelial and tumor cells via VEGF-enriched exosomes.http://dx.doi.org/10.1080/20013078.2019.1629865exosomesvascular endothelial growth factor (vegf)anti-angiogenesisanti-autophagypre-metastatic niches |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ye Zeng Xinghong Yao Xiaoheng Liu Xueling He Liang Li Xiaojing Liu Zhiping Yan Jiang Wu Bingmei M. Fu |
spellingShingle |
Ye Zeng Xinghong Yao Xiaoheng Liu Xueling He Liang Li Xiaojing Liu Zhiping Yan Jiang Wu Bingmei M. Fu Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis Journal of Extracellular Vesicles exosomes vascular endothelial growth factor (vegf) anti-angiogenesis anti-autophagy pre-metastatic niches |
author_facet |
Ye Zeng Xinghong Yao Xiaoheng Liu Xueling He Liang Li Xiaojing Liu Zhiping Yan Jiang Wu Bingmei M. Fu |
author_sort |
Ye Zeng |
title |
Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis |
title_short |
Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis |
title_full |
Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis |
title_fullStr |
Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis |
title_full_unstemmed |
Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis |
title_sort |
anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis |
publisher |
Taylor & Francis Group |
series |
Journal of Extracellular Vesicles |
issn |
2001-3078 |
publishDate |
2019-12-01 |
description |
Although anti-angiogenic therapies (AATs) have some effects against multiple malignancies, they are limited by subsequent tumor vasculogenesis and progression. To investigate the mechanisms by which tumor vasculogenesis and progression following AATs, we transfected microRNA (miR)-9 into human umbilical vein endothelial cells (HUVECs) to mimic the tumor-associated endothelial cells in hepatocellular carcinoma and simulated the AATs in vitro and in vivo. We found that administration of the angiogenesis inhibitor vandetanib completely abolished miR-9-induced angiogenesis and promoted autophagy in HUVECs, but induced the release of vascular endothelial growth factor (VEGF)-enriched exosomes. These VEGF-enriched exosomes significantly promoted the formation of endothelial vessels and vasculogenic mimicry in hepatocellular carcinoma and its progression in mice. Anti-autophagic therapy is proposed to improve the efficacy of AATs. However, similar effects by AATs were observed with the application of anti-autophagy by 3-methyladenine. Our results revealed that tumor vasculogenesis and progression after AATs and anti-autophagic therapies were due to the cross-talk between endothelial and tumor cells via VEGF-enriched exosomes. |
topic |
exosomes vascular endothelial growth factor (vegf) anti-angiogenesis anti-autophagy pre-metastatic niches |
url |
http://dx.doi.org/10.1080/20013078.2019.1629865 |
work_keys_str_mv |
AT yezeng antiangiogenesistriggersexosomesreleasefromendothelialcellstopromotetumorvasculogenesis AT xinghongyao antiangiogenesistriggersexosomesreleasefromendothelialcellstopromotetumorvasculogenesis AT xiaohengliu antiangiogenesistriggersexosomesreleasefromendothelialcellstopromotetumorvasculogenesis AT xuelinghe antiangiogenesistriggersexosomesreleasefromendothelialcellstopromotetumorvasculogenesis AT liangli antiangiogenesistriggersexosomesreleasefromendothelialcellstopromotetumorvasculogenesis AT xiaojingliu antiangiogenesistriggersexosomesreleasefromendothelialcellstopromotetumorvasculogenesis AT zhipingyan antiangiogenesistriggersexosomesreleasefromendothelialcellstopromotetumorvasculogenesis AT jiangwu antiangiogenesistriggersexosomesreleasefromendothelialcellstopromotetumorvasculogenesis AT bingmeimfu antiangiogenesistriggersexosomesreleasefromendothelialcellstopromotetumorvasculogenesis |
_version_ |
1724812258961260544 |