Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics

Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics

Abstract Background The recently developed genetically encoded calcium indicator (GECI), called NTnC, has a novel design with reduced size due to utilization of the troponin C (TnC) as a Ca2+-binding moiety inserted into the mNeonGreen fluorescent protein. NTnC binds two times less Ca2+ ions while m...

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Bibliographic Details
Main Authors: D. A. Doronin, N. V. Barykina, O. M. Subach, V. P. Sotskov, V. V. Plusnin, O. A. Ivleva, E. A. Isaakova, A. M. Varizhuk, G. E. Pozmogova, A. Y. Malyshev, I. V. Smirnov, K. D. Piatkevich, K. V. Anokhin, G. N. Enikolopov, F. V. Subach
Format: Article
Language:English
Published: BMC 2018-02-01
Series:BMC Biotechnology
Subjects:
Calcium imaging
Genetically encoded calcium indicator
Protein engineering
Online Access:http://link.springer.com/article/10.1186/s12896-018-0417-2

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