Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains
We determined complete genome sequences of lineage 2 West Nile virus (WNV) strains isolated from patients in South Africa who had mild or severe WNV infections. These strains had previously been shown to produce either highly or less neuroinvasive infection and induced genes similar to corresponding...
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doaj-0a0fa8ca667941669f3fc370cb43fd512020-11-25T02:46:33ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592008-02-0114222223010.3201/eid1402.070457Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus StrainsElizabeth M. BothaWanda MarkotterMariaan WolfaardtJanusz T. PaweskaRobert SwanepoelGustavio PalaciosLouis H. NelMarietjie VenterWe determined complete genome sequences of lineage 2 West Nile virus (WNV) strains isolated from patients in South Africa who had mild or severe WNV infections. These strains had previously been shown to produce either highly or less neuroinvasive infection and induced genes similar to corresponding highly or less neuroinvasive lineage 1 strains in mice. Phylogenetic and amino acid comparison of highly and less neuroinvasive lineage 2 strains demonstrated that the nonstructural genes, especially the nonstructural protein 5 gene, were most variable. All South African lineage 2 strains possessed the envelope-protein glycosylation site previously postulated to be associated with virulence. Major deletions existed in the 3′ noncoding region of 2 lineage 2 strains previously shown to be either less or not neuroinvasive relative to the highly neuroinvasive strains sequenced in this study.https://wwwnc.cdc.gov/eid/article/14/2/07-0457_articleWest Nile virusflavivirusphylogenetic analysisgenome sequence analysisstrain virulenceneuroinvasive |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Elizabeth M. Botha Wanda Markotter Mariaan Wolfaardt Janusz T. Paweska Robert Swanepoel Gustavio Palacios Louis H. Nel Marietjie Venter |
spellingShingle |
Elizabeth M. Botha Wanda Markotter Mariaan Wolfaardt Janusz T. Paweska Robert Swanepoel Gustavio Palacios Louis H. Nel Marietjie Venter Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains Emerging Infectious Diseases West Nile virus flavivirus phylogenetic analysis genome sequence analysis strain virulence neuroinvasive |
author_facet |
Elizabeth M. Botha Wanda Markotter Mariaan Wolfaardt Janusz T. Paweska Robert Swanepoel Gustavio Palacios Louis H. Nel Marietjie Venter |
author_sort |
Elizabeth M. Botha |
title |
Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains |
title_short |
Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains |
title_full |
Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains |
title_fullStr |
Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains |
title_full_unstemmed |
Genetic Determinants of Virulence in Pathogenic Lineage 2 West Nile Virus Strains |
title_sort |
genetic determinants of virulence in pathogenic lineage 2 west nile virus strains |
publisher |
Centers for Disease Control and Prevention |
series |
Emerging Infectious Diseases |
issn |
1080-6040 1080-6059 |
publishDate |
2008-02-01 |
description |
We determined complete genome sequences of lineage 2 West Nile virus (WNV) strains isolated from patients in South Africa who had mild or severe WNV infections. These strains had previously been shown to produce either highly or less neuroinvasive infection and induced genes similar to corresponding highly or less neuroinvasive lineage 1 strains in mice. Phylogenetic and amino acid comparison of highly and less neuroinvasive lineage 2 strains demonstrated that the nonstructural genes, especially the nonstructural protein 5 gene, were most variable. All South African lineage 2 strains possessed the envelope-protein glycosylation site previously postulated to be associated with virulence. Major deletions existed in the 3′ noncoding region of 2 lineage 2 strains previously shown to be either less or not neuroinvasive relative to the highly neuroinvasive strains sequenced in this study. |
topic |
West Nile virus flavivirus phylogenetic analysis genome sequence analysis strain virulence neuroinvasive |
url |
https://wwwnc.cdc.gov/eid/article/14/2/07-0457_article |
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