Dengue NS1 Antigen - for Early Detection of Dengue Virus Infection
Objectives: To evaluate the efficacy of NS1 antigen assay for early diagnosis of dengue virus infection in a tertiary care hospital. Methods: This cross sectional study was carried out in department of Medicine from August to December 2013. Total 100 patients with dengue fever were included. C...
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BKL Walawalkar Rural Medical College & Hospital
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doaj-0a217e576c264a4d82bf6d31b2fd08b02020-11-25T01:28:53ZengBKL Walawalkar Rural Medical College & HospitalWalawalkar International Medical Journal2349-29102395-06842015-08-01211115Dengue NS1 Antigen - for Early Detection of Dengue Virus InfectionAmol Hartalkar0Sheetal Hartalkar1Makarand Wani 2Arvind Yadav3Associate Professor of General Medicine, Rural Medical College, Pravara Institute of Medical Sciences (DU), P.O. LoniAssistant Professor,Rural Dental College, Pravara Institute of Medical Sciences (DU), P.O. Loni 2Resident of General Medicine, Smt. Kashibai Navale Medical College, Narhe, Pune Professor of Biochemistry, B.K.L. Walawalkar Rural Medical College & Hospital, Sawarde, Tal. Chiplun,. Dist. Ratnagiri .Objectives: To evaluate the efficacy of NS1 antigen assay for early diagnosis of dengue virus infection in a tertiary care hospital. Methods: This cross sectional study was carried out in department of Medicine from August to December 2013. Total 100 patients with dengue fever were included. Complete blood count, alanine aminotransferase (ALT), aspartate aminotransferase (AST), Dengue NS1 antigen and IgM and IgG antibodies of dengue virus were done in all cases. Results: Of the 100 sera tested, 75% were positive for dengue virus infection based on dengue NS1 antigen, IgM antibody and IgG antibody. Dengue NS1 antigen and IgM, IgG antibody were able to detect dengue virus infection between day 1 to day 8 in 92% of samples, 86.7% of samples and 82.6% of samples respectively. Sixty nine percent (69%) were found positive for dengue NS1 antigen, 65% were IgM positive and 62% were IgG positive. Based on the dengue NS1 antigen and IgM antibody combination, 74% were positive for dengue virus infections. Sensitivity of Dengue NS1 antigen was 92.3% and specificity of 74.28% in comparison to IgM antibody. Detection rate increased to 75%, based on the antigen and IgG antibody combination. Sensitivity of dengue NS1 antigen was 90.3% and specificity of 65.8% in comparison to IgG antibody. Conclusion: Dengue NS1 antigen is a useful, sensitive and specific test for early diagnosis of dengue virus infection and it improves diagnostic efficiency in combination with antibody test. Key words: Dengue fever, NS1 antigen. Introduction: Dengue fever (DF) is the most common arboviral illness in humans. Each year, an estimated 50-100 million cases of dengue fever and 500,000 cases of dengue hemorrhagic fever occur worldwide, with 30000 deaths (mainly in children). Globally 2.5-3 billion people in approximately 112 tropical and subtropical countries are at risk of dengue.of samples respectively. Sixty nine percent (69%) were found positive for dengue NS1 antigen, 65% were IgM positive and 62% were IgG positive. Based on the dengue NS1 antigen and IgM antibody combination, 74% were positive for dengue virus infections. Sensitivity of Dengue NS1 antigen was 92.3% and specificity of 74.28% in comparison to IgM antibody. Detection rate increased to 75%, based on the antigen and IgG antibody combination. Sensitivity of dengue NS1 antigen was 90.3% and specificity of 65.8% in comparison to IgG antibody. Conclusion: Dengue NS1 antigen is a useful, sensitive and specific test for early diagnosis of dengue virus infection and it improves diagnostic efficiency in combination with antibody test. http://www.wimjournal.com/html/journal/images/2.pdfDengue feverNS1 antigen. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amol Hartalkar Sheetal Hartalkar Makarand Wani Arvind Yadav |
spellingShingle |
Amol Hartalkar Sheetal Hartalkar Makarand Wani Arvind Yadav Dengue NS1 Antigen - for Early Detection of Dengue Virus Infection Walawalkar International Medical Journal Dengue fever NS1 antigen. |
author_facet |
Amol Hartalkar Sheetal Hartalkar Makarand Wani Arvind Yadav |
author_sort |
Amol Hartalkar |
title |
Dengue NS1 Antigen - for Early Detection of Dengue Virus Infection |
title_short |
Dengue NS1 Antigen - for Early Detection of Dengue Virus Infection |
title_full |
Dengue NS1 Antigen - for Early Detection of Dengue Virus Infection |
title_fullStr |
Dengue NS1 Antigen - for Early Detection of Dengue Virus Infection |
title_full_unstemmed |
Dengue NS1 Antigen - for Early Detection of Dengue Virus Infection |
title_sort |
dengue ns1 antigen - for early detection of dengue virus infection |
publisher |
BKL Walawalkar Rural Medical College & Hospital |
series |
Walawalkar International Medical Journal |
issn |
2349-2910 2395-0684 |
publishDate |
2015-08-01 |
description |
Objectives: To evaluate the efficacy of NS1 antigen
assay for early diagnosis of dengue virus infection in
a tertiary care hospital.
Methods: This cross sectional study was carried out
in department of Medicine from August to December
2013. Total 100 patients with dengue fever were
included. Complete blood count, alanine
aminotransferase (ALT), aspartate aminotransferase
(AST), Dengue NS1 antigen and IgM and IgG
antibodies of dengue virus were done in all cases.
Results: Of the 100 sera tested, 75% were positive
for dengue virus infection based on dengue NS1
antigen, IgM antibody and IgG antibody. Dengue
NS1 antigen and IgM, IgG antibody were able to
detect dengue virus infection between day 1 to day
8 in 92% of samples, 86.7% of samples and 82.6%
of samples respectively. Sixty nine percent (69%)
were found positive for dengue NS1 antigen, 65%
were IgM positive and 62% were IgG positive.
Based on the dengue NS1 antigen and IgM
antibody combination, 74% were positive for
dengue virus infections. Sensitivity of Dengue NS1
antigen was 92.3% and specificity of 74.28% in
comparison to IgM antibody. Detection rate
increased to 75%, based on the antigen and IgG
antibody combination. Sensitivity of dengue NS1
antigen was 90.3% and specificity of 65.8% in
comparison to IgG antibody.
Conclusion: Dengue NS1 antigen is a useful,
sensitive and specific test for early diagnosis of
dengue virus infection and it improves diagnostic
efficiency in combination with antibody test.
Key words: Dengue fever, NS1 antigen.
Introduction:
Dengue fever (DF) is the most common arboviral
illness in humans. Each year, an estimated 50-100
million cases of dengue fever and 500,000 cases of
dengue hemorrhagic fever occur worldwide, with
30000 deaths (mainly in children). Globally 2.5-3
billion people in approximately 112 tropical and
subtropical countries are at risk of dengue.of samples respectively. Sixty nine percent (69%)
were found positive for dengue NS1 antigen, 65%
were IgM positive and 62% were IgG positive.
Based on the dengue NS1 antigen and IgM
antibody combination, 74% were positive for
dengue virus infections. Sensitivity of Dengue NS1
antigen was 92.3% and specificity of 74.28% in
comparison to IgM antibody. Detection rate
increased to 75%, based on the antigen and IgG
antibody combination. Sensitivity of dengue NS1
antigen was 90.3% and specificity of 65.8% in
comparison to IgG antibody.
Conclusion: Dengue NS1 antigen is a useful,
sensitive and specific test for early diagnosis of
dengue virus infection and it improves diagnostic
efficiency in combination with antibody test.
|
topic |
Dengue fever NS1 antigen. |
url |
http://www.wimjournal.com/html/journal/images/2.pdf |
work_keys_str_mv |
AT amolhartalkar denguens1antigenforearlydetectionofdenguevirusinfection AT sheetalhartalkar denguens1antigenforearlydetectionofdenguevirusinfection AT makarandwani denguens1antigenforearlydetectionofdenguevirusinfection AT arvindyadav denguens1antigenforearlydetectionofdenguevirusinfection |
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