Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence
Tempeh is a type of traditional fermented food in Indonesia. The fermentation can be performed by Rhizopus microsporus as a main microorganism. However, Bacillus spp. is found in abundance in tempeh production. Nevertheless, information regarding the diversity of Bacillus spp. in tempeh production h...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Bogor Agricultural University
2017-01-01
|
Series: | Hayati Journal of Biosciences |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916300390 |
id |
doaj-0a2939cc70964c2e9069fff4befb070e |
---|---|
record_format |
Article |
spelling |
doaj-0a2939cc70964c2e9069fff4befb070e2020-11-24T21:13:45ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192017-01-01241354010.1016/j.hjb.2017.05.001Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene SequenceTati Barus0Linda Wati1Melani2Antonius Suwanto3Yogiara4Atma Jaya Catholic University of Indonesia, Jakarta 12930, IndonesiaAtma Jaya Catholic University of Indonesia, Jakarta 12930, IndonesiaAtma Jaya Catholic University of Indonesia, Jakarta 12930, IndonesiaBogor Agricultural University, Bogor 16680, West Java, IndonesiaAtma Jaya Catholic University of Indonesia, Jakarta 12930, IndonesiaTempeh is a type of traditional fermented food in Indonesia. The fermentation can be performed by Rhizopus microsporus as a main microorganism. However, Bacillus spp. is found in abundance in tempeh production. Nevertheless, information regarding the diversity of Bacillus spp. in tempeh production has not been reported yet. Therefore, the aim of this investigation was to study the genetic diversity of Bacillus spp. in tempeh production based on the 16S ribosomal RNA sequence. In this study, about 22 of 24 fresh tempeh from Jakarta, Bogor, and Tangerang were used. A total of 52 protease-producing Bacillus spp. isolates were obtained. Based on 16S ribosomal RNA results, all 52 isolates were identified to be similar to B. pumilus, B. subtilis, B. megaterium, B. licheniformis, B. cereus, B. thuringiensis, B. amyloliquefaciens, Brevibacillus brevis, and Bacillus sp. All the identified isolates were divided into two large clusters: 1) a cluster of B. cereus, B. thuringiensis, Bacillus sp., and B. brevis and 2) a cluster of B. pumilus, B. subtilis, B. megaterium, B. licheniformis, and B. amyloliquefaciens. Information about the Bacillus spp. role in determining the quality of tempeh has not been reported and this is a preliminary study of Bacillus spp. from tempeh.http://www.sciencedirect.com/science/article/pii/S1978301916300390Bacillus sppproteaseRhizopus microsporustempeh |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tati Barus Linda Wati Melani Antonius Suwanto Yogiara |
spellingShingle |
Tati Barus Linda Wati Melani Antonius Suwanto Yogiara Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence Hayati Journal of Biosciences Bacillus spp protease Rhizopus microsporus tempeh |
author_facet |
Tati Barus Linda Wati Melani Antonius Suwanto Yogiara |
author_sort |
Tati Barus |
title |
Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence |
title_short |
Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence |
title_full |
Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence |
title_fullStr |
Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence |
title_full_unstemmed |
Diversity of Protease-Producing Bacillus spp. From Fresh Indonesian Tempeh Based on 16S rRNA Gene Sequence |
title_sort |
diversity of protease-producing bacillus spp. from fresh indonesian tempeh based on 16s rrna gene sequence |
publisher |
Bogor Agricultural University |
series |
Hayati Journal of Biosciences |
issn |
1978-3019 |
publishDate |
2017-01-01 |
description |
Tempeh is a type of traditional fermented food in Indonesia. The fermentation can be performed by Rhizopus microsporus as a main microorganism. However, Bacillus spp. is found in abundance in tempeh production. Nevertheless, information regarding the diversity of Bacillus spp. in tempeh production has not been reported yet. Therefore, the aim of this investigation was to study the genetic diversity of Bacillus spp. in tempeh production based on the 16S ribosomal RNA sequence. In this study, about 22 of 24 fresh tempeh from Jakarta, Bogor, and Tangerang were used. A total of 52 protease-producing Bacillus spp. isolates were obtained. Based on 16S ribosomal RNA results, all 52 isolates were identified to be similar to B. pumilus, B. subtilis, B. megaterium, B. licheniformis, B. cereus, B. thuringiensis, B. amyloliquefaciens, Brevibacillus brevis, and Bacillus sp. All the identified isolates were divided into two large clusters: 1) a cluster of B. cereus, B. thuringiensis, Bacillus sp., and B. brevis and 2) a cluster of B. pumilus, B. subtilis, B. megaterium, B. licheniformis, and B. amyloliquefaciens. Information about the Bacillus spp. role in determining the quality of tempeh has not been reported and this is a preliminary study of Bacillus spp. from tempeh. |
topic |
Bacillus spp protease Rhizopus microsporus tempeh |
url |
http://www.sciencedirect.com/science/article/pii/S1978301916300390 |
work_keys_str_mv |
AT tatibarus diversityofproteaseproducingbacillussppfromfreshindonesiantempehbasedon16srrnagenesequence AT lindawati diversityofproteaseproducingbacillussppfromfreshindonesiantempehbasedon16srrnagenesequence AT melani diversityofproteaseproducingbacillussppfromfreshindonesiantempehbasedon16srrnagenesequence AT antoniussuwanto diversityofproteaseproducingbacillussppfromfreshindonesiantempehbasedon16srrnagenesequence AT yogiara diversityofproteaseproducingbacillussppfromfreshindonesiantempehbasedon16srrnagenesequence |
_version_ |
1716748376713723905 |