Septin-Containing Barriers Control the Differential Inheritance of Cytoplasmic Elements
Fusion of haploid cells of Saccharomyces cerevisiae generates zygotes. We observe that the zygote midzone includes a septin annulus and differentially affects redistribution of supramolecular complexes and organelles. Redistribution across the midzone of supramolecular complexes (polysomes and Sup3...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2013-01-01
|
Series: | Cell Reports |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124712004196 |
id |
doaj-0a366b44e5cf432b86ee12a93514cdec |
---|---|
record_format |
Article |
spelling |
doaj-0a366b44e5cf432b86ee12a93514cdec2020-11-24T21:43:40ZengElsevierCell Reports2211-12472013-01-013122323610.1016/j.celrep.2012.11.022Septin-Containing Barriers Control the Differential Inheritance of Cytoplasmic ElementsAlan Michael Tartakoff0Ilya Aylyarov1Purnima Jaiswal2Pathology Department and Cell Biology Program, Case Western Reserve University, 2103 Cornell Road, Cleveland, OH 44106, USAPathology Department and Cell Biology Program, Case Western Reserve University, 2103 Cornell Road, Cleveland, OH 44106, USAPathology Department and Cell Biology Program, Case Western Reserve University, 2103 Cornell Road, Cleveland, OH 44106, USA Fusion of haploid cells of Saccharomyces cerevisiae generates zygotes. We observe that the zygote midzone includes a septin annulus and differentially affects redistribution of supramolecular complexes and organelles. Redistribution across the midzone of supramolecular complexes (polysomes and Sup35p-GFP [PSI+]) is unexpectedly delayed relative to soluble proteins; however, in [psi-] × [PSI+] crosses, all buds eventually receive Sup35p-GFP [PSI+]. Encounter between parental mitochondria is further delayed until septins relocate to the bud site, where they are required for repolarization of the actin cytoskeleton. This delay allows rationalization of the longstanding observation that terminal zygotic buds preferentially inherit a single mitochondrial genotype. The rate of redistribution of complexes and organelles determines whether their inheritance will be uniform. http://www.sciencedirect.com/science/article/pii/S2211124712004196 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alan Michael Tartakoff Ilya Aylyarov Purnima Jaiswal |
spellingShingle |
Alan Michael Tartakoff Ilya Aylyarov Purnima Jaiswal Septin-Containing Barriers Control the Differential Inheritance of Cytoplasmic Elements Cell Reports |
author_facet |
Alan Michael Tartakoff Ilya Aylyarov Purnima Jaiswal |
author_sort |
Alan Michael Tartakoff |
title |
Septin-Containing Barriers Control the Differential Inheritance of Cytoplasmic Elements |
title_short |
Septin-Containing Barriers Control the Differential Inheritance of Cytoplasmic Elements |
title_full |
Septin-Containing Barriers Control the Differential Inheritance of Cytoplasmic Elements |
title_fullStr |
Septin-Containing Barriers Control the Differential Inheritance of Cytoplasmic Elements |
title_full_unstemmed |
Septin-Containing Barriers Control the Differential Inheritance of Cytoplasmic Elements |
title_sort |
septin-containing barriers control the differential inheritance of cytoplasmic elements |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2013-01-01 |
description |
Fusion of haploid cells of Saccharomyces cerevisiae generates zygotes. We observe that the zygote midzone includes a septin annulus and differentially affects redistribution of supramolecular complexes and organelles. Redistribution across the midzone of supramolecular complexes (polysomes and Sup35p-GFP [PSI+]) is unexpectedly delayed relative to soluble proteins; however, in [psi-] × [PSI+] crosses, all buds eventually receive Sup35p-GFP [PSI+]. Encounter between parental mitochondria is further delayed until septins relocate to the bud site, where they are required for repolarization of the actin cytoskeleton. This delay allows rationalization of the longstanding observation that terminal zygotic buds preferentially inherit a single mitochondrial genotype. The rate of redistribution of complexes and organelles determines whether their inheritance will be uniform.
|
url |
http://www.sciencedirect.com/science/article/pii/S2211124712004196 |
work_keys_str_mv |
AT alanmichaeltartakoff septincontainingbarrierscontrolthedifferentialinheritanceofcytoplasmicelements AT ilyaaylyarov septincontainingbarrierscontrolthedifferentialinheritanceofcytoplasmicelements AT purnimajaiswal septincontainingbarrierscontrolthedifferentialinheritanceofcytoplasmicelements |
_version_ |
1725912669656973312 |