Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in Croatia
Mycobacterium tuberculosis remains a major global health problem and is currently killing 1.5 million people every year. One of the most important steps in tuberculosis control is the rapid and accurate laboratory diagnosis. The Xpert MTB/RIF assay is a novel molecular, easy-to-use assay, which can...
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Wolters Kluwer Medknow Publications
2013-01-01
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doaj-0a4f94bf33d34f949ef602d6939227762020-11-24T23:21:39ZengWolters Kluwer Medknow PublicationsInternational Journal of Mycobacteriology2212-55312212-554X2013-01-012317918210.1016/j.ijmyco.2013.05.003Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in CroatiaLjiljana ZmakMateja JankovicVera Katalinic JankovicMycobacterium tuberculosis remains a major global health problem and is currently killing 1.5 million people every year. One of the most important steps in tuberculosis control is the rapid and accurate laboratory diagnosis. The Xpert MTB/RIF assay is a novel molecular, easy-to-use assay, which can lead to tuberculosis identification in less than 2 h. In this study, the Xpert MTB/RIF assay performance for rapid diagnosis of tuberculosis was evaluated in comparison with conventional culture methods; 361 pulmonary and extrapulmonary patient samples were collected between October 2010 and October 2012 and were analyzed at the National Reference laboratory for Mycobacteria, Zagreb, Croatia. For pulmonary samples the sensitivity and specificity were 86% and 100%, while for extrapulmonary samples the sensitivity and specificity were 75% and 99%, respectively. It was concluded that Xpert MTB/RIF assay has high sensitivity and specificity for both pulmonary and extrapulmonary specimens.http://www.ijmyco.org/article.asp?issn=2212-5531;year=2013;volume=2;issue=3;spage=179;epage=182;aulast=ZmakXpert MTB/RIF assayTuberculosisMolecular methodsRapid diagnosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ljiljana Zmak Mateja Jankovic Vera Katalinic Jankovic |
spellingShingle |
Ljiljana Zmak Mateja Jankovic Vera Katalinic Jankovic Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in Croatia International Journal of Mycobacteriology Xpert MTB/RIF assay Tuberculosis Molecular methods Rapid diagnosis |
author_facet |
Ljiljana Zmak Mateja Jankovic Vera Katalinic Jankovic |
author_sort |
Ljiljana Zmak |
title |
Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in Croatia |
title_short |
Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in Croatia |
title_full |
Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in Croatia |
title_fullStr |
Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in Croatia |
title_full_unstemmed |
Evaluation of Xpert MTB/RIF assay for rapid molecular diagnosis of tuberculosis in a two-year period in Croatia |
title_sort |
evaluation of xpert mtb/rif assay for rapid molecular diagnosis of tuberculosis in a two-year period in croatia |
publisher |
Wolters Kluwer Medknow Publications |
series |
International Journal of Mycobacteriology |
issn |
2212-5531 2212-554X |
publishDate |
2013-01-01 |
description |
Mycobacterium tuberculosis remains a major global health problem and is currently killing 1.5 million people every year. One of the most important steps in tuberculosis control is the rapid and accurate laboratory diagnosis. The Xpert MTB/RIF assay is a novel molecular, easy-to-use assay, which can lead to tuberculosis identification in less than 2 h. In this study, the Xpert MTB/RIF assay performance for rapid diagnosis of tuberculosis was evaluated in comparison with conventional culture methods; 361 pulmonary and extrapulmonary patient samples were collected between October 2010 and October 2012 and were analyzed at the National Reference laboratory for Mycobacteria, Zagreb, Croatia. For pulmonary samples the sensitivity and specificity were 86% and 100%, while for extrapulmonary samples the sensitivity and specificity were 75% and 99%, respectively. It was concluded that Xpert MTB/RIF assay has high sensitivity and specificity for both pulmonary and extrapulmonary specimens. |
topic |
Xpert MTB/RIF assay Tuberculosis Molecular methods Rapid diagnosis |
url |
http://www.ijmyco.org/article.asp?issn=2212-5531;year=2013;volume=2;issue=3;spage=179;epage=182;aulast=Zmak |
work_keys_str_mv |
AT ljiljanazmak evaluationofxpertmtbrifassayforrapidmoleculardiagnosisoftuberculosisinatwoyearperiodincroatia AT matejajankovic evaluationofxpertmtbrifassayforrapidmoleculardiagnosisoftuberculosisinatwoyearperiodincroatia AT verakatalinicjankovic evaluationofxpertmtbrifassayforrapidmoleculardiagnosisoftuberculosisinatwoyearperiodincroatia |
_version_ |
1725570861298089984 |