RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected With Mycobacterium bovis Across an Experimental Time Course
Bovine tuberculosis, caused by infection with members of the Mycobacterium tuberculosis complex, particularly Mycobacterium bovis, is a major endemic disease affecting cattle populations worldwide, despite the implementation of stringent surveillance and control programs in many countries. The devel...
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Frontiers Media S.A.
2021-05-01
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Series: | Frontiers in Veterinary Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.662002/full |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kirsten E. McLoughlin Carolina N. Correia John A. Browne David A. Magee Nicolas C. Nalpas Kevin Rue-Albrecht Adam O. Whelan Bernardo Villarreal-Ramos H. Martin Vordermeier Eamonn Gormley Stephen V. Gordon Stephen V. Gordon David E. MacHugh David E. MacHugh |
spellingShingle |
Kirsten E. McLoughlin Carolina N. Correia John A. Browne David A. Magee Nicolas C. Nalpas Kevin Rue-Albrecht Adam O. Whelan Bernardo Villarreal-Ramos H. Martin Vordermeier Eamonn Gormley Stephen V. Gordon Stephen V. Gordon David E. MacHugh David E. MacHugh RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected With Mycobacterium bovis Across an Experimental Time Course Frontiers in Veterinary Science biomarker cattle gene expression host-pathogen interaction immune response time series |
author_facet |
Kirsten E. McLoughlin Carolina N. Correia John A. Browne David A. Magee Nicolas C. Nalpas Kevin Rue-Albrecht Adam O. Whelan Bernardo Villarreal-Ramos H. Martin Vordermeier Eamonn Gormley Stephen V. Gordon Stephen V. Gordon David E. MacHugh David E. MacHugh |
author_sort |
Kirsten E. McLoughlin |
title |
RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected With Mycobacterium bovis Across an Experimental Time Course |
title_short |
RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected With Mycobacterium bovis Across an Experimental Time Course |
title_full |
RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected With Mycobacterium bovis Across an Experimental Time Course |
title_fullStr |
RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected With Mycobacterium bovis Across an Experimental Time Course |
title_full_unstemmed |
RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected With Mycobacterium bovis Across an Experimental Time Course |
title_sort |
rna-seq transcriptome analysis of peripheral blood from cattle infected with mycobacterium bovis across an experimental time course |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2021-05-01 |
description |
Bovine tuberculosis, caused by infection with members of the Mycobacterium tuberculosis complex, particularly Mycobacterium bovis, is a major endemic disease affecting cattle populations worldwide, despite the implementation of stringent surveillance and control programs in many countries. The development of high-throughput functional genomics technologies, including RNA sequencing, has enabled detailed analysis of the host transcriptome to M. bovis infection, particularly at the macrophage and peripheral blood level. In the present study, we have analysed the transcriptome of bovine whole peripheral blood samples collected at −1 week pre-infection and +1, +2, +6, +10, and +12 weeks post-infection time points. Differentially expressed genes were catalogued and evaluated at each post-infection time point relative to the −1 week pre-infection time point and used for the identification of putative candidate host transcriptional biomarkers for M. bovis infection. Differentially expressed gene sets were also used for examination of cellular pathways associated with the host response to M. bovis infection, construction of de novo gene interaction networks enriched for host differentially expressed genes, and time-series analyses to identify functionally important groups of genes displaying similar patterns of expression across the infection time course. A notable outcome of these analyses was identification of a 19-gene transcriptional biosignature of infection consisting of genes increased in expression across the time course from +1 week to +12 weeks post-infection. |
topic |
biomarker cattle gene expression host-pathogen interaction immune response time series |
url |
https://www.frontiersin.org/articles/10.3389/fvets.2021.662002/full |
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doaj-0a59da780d9844058e5ac7e541456e2c2021-05-28T07:06:31ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-05-01810.3389/fvets.2021.662002662002RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected With Mycobacterium bovis Across an Experimental Time CourseKirsten E. McLoughlin0Carolina N. Correia1John A. Browne2David A. Magee3Nicolas C. Nalpas4Kevin Rue-Albrecht5Adam O. Whelan6Bernardo Villarreal-Ramos7H. Martin Vordermeier8Eamonn Gormley9Stephen V. Gordon10Stephen V. Gordon11David E. MacHugh12David E. MacHugh13Animal Genomics Laboratory, UCD School of Agriculture and Food Science, UCD College of Health and Agricultural Sciences, University College Dublin, Dublin, IrelandAnimal Genomics Laboratory, UCD School of Agriculture and Food Science, UCD College of Health and Agricultural Sciences, University College Dublin, Dublin, IrelandAnimal Genomics Laboratory, UCD School of Agriculture and Food Science, UCD College of Health and Agricultural Sciences, University College Dublin, Dublin, IrelandAnimal Genomics Laboratory, UCD School of Agriculture and Food Science, UCD College of Health and Agricultural Sciences, University College Dublin, Dublin, IrelandAnimal Genomics Laboratory, UCD School of Agriculture and Food Science, UCD College of Health and Agricultural Sciences, University College Dublin, Dublin, IrelandAnimal Genomics Laboratory, UCD School of Agriculture and Food Science, UCD College of Health and Agricultural Sciences, University College Dublin, Dublin, IrelandTB Immunology and Vaccinology Team, Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United KingdomTB Immunology and Vaccinology Team, Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United KingdomTB Immunology and Vaccinology Team, Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United KingdomUCD School of Veterinary Medicine, UCD College of Health and Agricultural Sciences, University College Dublin, Dublin, IrelandUCD School of Veterinary Medicine, UCD College of Health and Agricultural Sciences, University College Dublin, Dublin, IrelandUCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, IrelandAnimal Genomics Laboratory, UCD School of Agriculture and Food Science, UCD College of Health and Agricultural Sciences, University College Dublin, Dublin, IrelandUCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, IrelandBovine tuberculosis, caused by infection with members of the Mycobacterium tuberculosis complex, particularly Mycobacterium bovis, is a major endemic disease affecting cattle populations worldwide, despite the implementation of stringent surveillance and control programs in many countries. The development of high-throughput functional genomics technologies, including RNA sequencing, has enabled detailed analysis of the host transcriptome to M. bovis infection, particularly at the macrophage and peripheral blood level. In the present study, we have analysed the transcriptome of bovine whole peripheral blood samples collected at −1 week pre-infection and +1, +2, +6, +10, and +12 weeks post-infection time points. Differentially expressed genes were catalogued and evaluated at each post-infection time point relative to the −1 week pre-infection time point and used for the identification of putative candidate host transcriptional biomarkers for M. bovis infection. Differentially expressed gene sets were also used for examination of cellular pathways associated with the host response to M. bovis infection, construction of de novo gene interaction networks enriched for host differentially expressed genes, and time-series analyses to identify functionally important groups of genes displaying similar patterns of expression across the infection time course. A notable outcome of these analyses was identification of a 19-gene transcriptional biosignature of infection consisting of genes increased in expression across the time course from +1 week to +12 weeks post-infection.https://www.frontiersin.org/articles/10.3389/fvets.2021.662002/fullbiomarkercattlegene expressionhost-pathogen interactionimmune responsetime series |