Evaluation of the Effect of Silver Nanoparticles on Induction of Neutraliz-ing Antibodies against Inactivated Rabies Virus
Background: Nanoparticles have been considered as promising tools because of their high applicability. Recently, nanoparticles have been evaluated for their ability to increase the immune responses as adjuvants. Silver-nanoparticles (AgNPs) have shown promising results in enhancing Th-2 immune respo...
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doaj-0a5f832ccb3c4f8f94caee5a66bd00482020-11-25T03:07:57ZengPasteur Institute of IranVaccine Research2383-28192423-49232014-08-01113134Evaluation of the Effect of Silver Nanoparticles on Induction of Neutraliz-ing Antibodies against Inactivated Rabies VirusVahid Asgary0Omid Kord Mafi1Mohammad Sadeq Khosravy2Alireza Janani3Nabiollah Namvar Asl4Rouzbeh Bashar5Hadi Poortaghi6Hossein Ahangari Cohan7Alireza Shoari8Reza Ahangari Cohan9 Department of Rabies, Virology Research Group, Pasteur Institute of Iran, Tehran, Iran, Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Department of Biotechnology, Payam-e-Nour University, East Branch, Tehran, Iran Department of Laboratory of Animal Sciences, Pasteur Institute of Iran, Tehran, Iran Department of Rabies, Virology Research Group, Pasteur Institute of Iran, Tehran, Iran Department of Laboratory of Animal Sciences, Pasteur Institute of Iran, Tehran, Iran Department of Rabies, Virology Research Group, Pasteur Institute of Iran, Tehran, Iran Faculty of Veterinary Medicine, Islamic Azad University, Karaj, Iran Faculty of Veterinary Medicine, Islamic Azad University, Karaj, Iran Department of Molecular and Cellular Biology, Islamic Azad University, Pharmaceutical Sciences Branch, Tehran, Iran Department of Rabies, Virology Research Group, Pasteur Institute of Iran, Tehran, Iran Background: Nanoparticles have been considered as promising tools because of their high applicability. Recently, nanoparticles have been evaluated for their ability to increase the immune responses as adjuvants. Silver-nanoparticles (AgNPs) have shown promising results in enhancing Th-2 immune responses and to produce potent neutralizing antibodies. Neutralizing antibodies are considered as the main defense mechanism in pre- and post-exposure treatments of rabies disease. Therefore in this study, the effects of AgNPs in enhancing the immunogenicity of inactivated rabies virus were assessed. Materials and Methods: Different concentrations of AgNPs (0.2, 0.4, 0.6 and 0.8 mg/ml) were added to inactivated rabies virus. Mice were immunized by two intra-peritoneal injections of each concentration on days 1 and 7. The inactivated virus and Alum were used as negative and positive controls, respectively. Blood was collected from healthy and immunized mice, one week after the last immunization. Serum was isolated from each sample and the amounts of neutralizing antibodies were determined by Rapid Florence Focus Inhibition Test (RFFIT). The cytotoxicity of AgNPs was also assessed by in vitro MTT assay on J774A.1 cell line. Results: The results showed that 0.4, 0.6 and 0.8 mg/ml of AgNPs had significantly increased the immune responses compared to the control however, 0.2 mg/ml of AgNPs did not show a significant effect. No cytotoxicity was observed for 0.001 and 0.01 mg/ml concentrations of AgNPs but cell viability was decreased significantly at 0.1 mg/ml concentration. Conclusion: It was shown that the virus-loaded AgNPsat 0.4 mg/ml concentration could raise the neutralizing antibodies against rabies virus in mice, but their adverse effect on cell viability excludes their use as an adjuvant. Vac Res , 2014, 1 (1): 32-35http://vacres.pasteur.ac.ir/article-1-29-en.htmladjuvantsilver nanoparticlerabies vaccineneutralizing antibodies |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vahid Asgary Omid Kord Mafi Mohammad Sadeq Khosravy Alireza Janani Nabiollah Namvar Asl Rouzbeh Bashar Hadi Poortaghi Hossein Ahangari Cohan Alireza Shoari Reza Ahangari Cohan |
spellingShingle |
Vahid Asgary Omid Kord Mafi Mohammad Sadeq Khosravy Alireza Janani Nabiollah Namvar Asl Rouzbeh Bashar Hadi Poortaghi Hossein Ahangari Cohan Alireza Shoari Reza Ahangari Cohan Evaluation of the Effect of Silver Nanoparticles on Induction of Neutraliz-ing Antibodies against Inactivated Rabies Virus Vaccine Research adjuvant silver nanoparticle rabies vaccine neutralizing antibodies |
author_facet |
Vahid Asgary Omid Kord Mafi Mohammad Sadeq Khosravy Alireza Janani Nabiollah Namvar Asl Rouzbeh Bashar Hadi Poortaghi Hossein Ahangari Cohan Alireza Shoari Reza Ahangari Cohan |
author_sort |
Vahid Asgary |
title |
Evaluation of the Effect of Silver Nanoparticles on Induction of Neutraliz-ing Antibodies against Inactivated Rabies Virus |
title_short |
Evaluation of the Effect of Silver Nanoparticles on Induction of Neutraliz-ing Antibodies against Inactivated Rabies Virus |
title_full |
Evaluation of the Effect of Silver Nanoparticles on Induction of Neutraliz-ing Antibodies against Inactivated Rabies Virus |
title_fullStr |
Evaluation of the Effect of Silver Nanoparticles on Induction of Neutraliz-ing Antibodies against Inactivated Rabies Virus |
title_full_unstemmed |
Evaluation of the Effect of Silver Nanoparticles on Induction of Neutraliz-ing Antibodies against Inactivated Rabies Virus |
title_sort |
evaluation of the effect of silver nanoparticles on induction of neutraliz-ing antibodies against inactivated rabies virus |
publisher |
Pasteur Institute of Iran |
series |
Vaccine Research |
issn |
2383-2819 2423-4923 |
publishDate |
2014-08-01 |
description |
Background: Nanoparticles have been considered as promising tools because of their high applicability. Recently, nanoparticles have been evaluated for their ability to increase the immune responses as adjuvants. Silver-nanoparticles (AgNPs) have shown promising results in enhancing Th-2 immune responses and to produce potent neutralizing antibodies. Neutralizing antibodies are considered as the main defense mechanism in pre- and post-exposure treatments of rabies disease. Therefore in this study, the effects of AgNPs in enhancing the immunogenicity of inactivated rabies virus were assessed. Materials and Methods: Different concentrations of AgNPs (0.2, 0.4, 0.6 and 0.8 mg/ml) were added to inactivated rabies virus. Mice were immunized by two intra-peritoneal injections of each concentration on days 1 and 7. The inactivated virus and Alum were used as negative and positive controls, respectively. Blood was collected from healthy and immunized mice, one week after the last immunization. Serum was isolated from each sample and the amounts of neutralizing antibodies were determined by Rapid Florence Focus Inhibition Test (RFFIT). The cytotoxicity of AgNPs was also assessed by in vitro MTT assay on J774A.1 cell line. Results: The results showed that 0.4, 0.6 and 0.8 mg/ml of AgNPs had significantly increased the immune responses compared to the control however, 0.2 mg/ml of AgNPs did not show a significant effect. No cytotoxicity was observed for 0.001 and 0.01 mg/ml concentrations of AgNPs but cell viability was decreased significantly at 0.1 mg/ml concentration. Conclusion: It was shown that the virus-loaded AgNPsat 0.4 mg/ml concentration could raise the neutralizing antibodies against rabies virus in mice, but their adverse effect on cell viability excludes their use as an adjuvant. Vac Res , 2014, 1 (1): 32-35 |
topic |
adjuvant silver nanoparticle rabies vaccine neutralizing antibodies |
url |
http://vacres.pasteur.ac.ir/article-1-29-en.html |
work_keys_str_mv |
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