Fine mapping of the major anthracnose resistance QTL AnR GO 5 in Capsicum chinense ‘PBC932’
Abstract Background Colletotrichum species are the causal agents of anthracnose, a major disease affecting the yield and quality of pepper (Capsicum spp.). Colletotrichum scovillei is widespread in China, has strong pathogenicity and drug resistance, and causes anthracnose disease in pepper fruits t...
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doaj-0a61ab3e65c547dcaaa2d510a0ac7e572020-11-25T03:04:37ZengBMCBMC Plant Biology1471-22292020-05-012011810.1186/s12870-019-2115-1Fine mapping of the major anthracnose resistance QTL AnR GO 5 in Capsicum chinense ‘PBC932’Yuanyuan Zhao0Yiwei Liu1Zhenghai Zhang2Yacong Cao3Hailong Yu4Wenwen Ma5Baoxi Zhang6Risheng Wang7Jie Gao8Lihao Wang9Key Laboratory of Vegetable Genetics and Physiology of the China Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesKey Laboratory of Vegetable Genetics and Physiology of the China Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesKey Laboratory of Vegetable Genetics and Physiology of the China Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesKey Laboratory of Vegetable Genetics and Physiology of the China Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesKey Laboratory of Vegetable Genetics and Physiology of the China Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesKey Laboratory of Vegetable Genetics and Physiology of the China Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesKey Laboratory of Vegetable Genetics and Physiology of the China Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesVegetable Research Institute, Guangxi Academy of Agricultural SciencesCollege of Forestry and Horticulture, Xinjiang Agricultural UniversityKey Laboratory of Vegetable Genetics and Physiology of the China Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesAbstract Background Colletotrichum species are the causal agents of anthracnose, a major disease affecting the yield and quality of pepper (Capsicum spp.). Colletotrichum scovillei is widespread in China, has strong pathogenicity and drug resistance, and causes anthracnose disease in pepper fruits that severely reduces production. Previously, an anti-anthracnose locus AnR GO 5 was mapped to the P5 chromosome on the basis of analyses of fruit at the green mature stage. The aim of this study was to narrow down the interval of this locus and identify the gene responsible for conferring resistance. Results On the basis of results of re-sequencing of Capsicum chinense ‘PBC932’ and C. annuum ‘77013’, we developed Kompetitive allele-specific PCR (KASPar) markers and insertion–deletion (InDel) markers linked to AnR GO 5 at the green mature fruit stage and used them to construct a genetic linkage map (42 markers, 24.4 cM in length). Using data obtained in phenotypic and genotypic analyses of BC4S1, BC4S2, and BC4S3 populations, AnR GO 5 was located between the markers P5in-2266-404 and P5in-2268-978 within a physical distance of 164 kb. This region contained five genes, including CA05g17730. CA05g17730 encodes ‘R1C-3-like’ putative late blight resistance protein homologs. The transcript level of CA05g17730 differed between ‘PBC932’ and ‘77013’. The structure of the CA05g17730 gene also differed between ‘PBC932’ and ‘77013’. Conclusions We narrowed down the QTL interval to a region containing five genes. These results will be useful for further research on the mechanisms of resistance to anthracnose, and for marker assisted selection for anthracnose-resistant capsicum lines.http://link.springer.com/article/10.1186/s12870-019-2115-1PepperAnthracnoseFine mappingColletotrichum scovilleiQTL |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuanyuan Zhao Yiwei Liu Zhenghai Zhang Yacong Cao Hailong Yu Wenwen Ma Baoxi Zhang Risheng Wang Jie Gao Lihao Wang |
spellingShingle |
Yuanyuan Zhao Yiwei Liu Zhenghai Zhang Yacong Cao Hailong Yu Wenwen Ma Baoxi Zhang Risheng Wang Jie Gao Lihao Wang Fine mapping of the major anthracnose resistance QTL AnR GO 5 in Capsicum chinense ‘PBC932’ BMC Plant Biology Pepper Anthracnose Fine mapping Colletotrichum scovillei QTL |
author_facet |
Yuanyuan Zhao Yiwei Liu Zhenghai Zhang Yacong Cao Hailong Yu Wenwen Ma Baoxi Zhang Risheng Wang Jie Gao Lihao Wang |
author_sort |
Yuanyuan Zhao |
title |
Fine mapping of the major anthracnose resistance QTL AnR GO 5 in Capsicum chinense ‘PBC932’ |
title_short |
Fine mapping of the major anthracnose resistance QTL AnR GO 5 in Capsicum chinense ‘PBC932’ |
title_full |
Fine mapping of the major anthracnose resistance QTL AnR GO 5 in Capsicum chinense ‘PBC932’ |
title_fullStr |
Fine mapping of the major anthracnose resistance QTL AnR GO 5 in Capsicum chinense ‘PBC932’ |
title_full_unstemmed |
Fine mapping of the major anthracnose resistance QTL AnR GO 5 in Capsicum chinense ‘PBC932’ |
title_sort |
fine mapping of the major anthracnose resistance qtl anr go 5 in capsicum chinense ‘pbc932’ |
publisher |
BMC |
series |
BMC Plant Biology |
issn |
1471-2229 |
publishDate |
2020-05-01 |
description |
Abstract Background Colletotrichum species are the causal agents of anthracnose, a major disease affecting the yield and quality of pepper (Capsicum spp.). Colletotrichum scovillei is widespread in China, has strong pathogenicity and drug resistance, and causes anthracnose disease in pepper fruits that severely reduces production. Previously, an anti-anthracnose locus AnR GO 5 was mapped to the P5 chromosome on the basis of analyses of fruit at the green mature stage. The aim of this study was to narrow down the interval of this locus and identify the gene responsible for conferring resistance. Results On the basis of results of re-sequencing of Capsicum chinense ‘PBC932’ and C. annuum ‘77013’, we developed Kompetitive allele-specific PCR (KASPar) markers and insertion–deletion (InDel) markers linked to AnR GO 5 at the green mature fruit stage and used them to construct a genetic linkage map (42 markers, 24.4 cM in length). Using data obtained in phenotypic and genotypic analyses of BC4S1, BC4S2, and BC4S3 populations, AnR GO 5 was located between the markers P5in-2266-404 and P5in-2268-978 within a physical distance of 164 kb. This region contained five genes, including CA05g17730. CA05g17730 encodes ‘R1C-3-like’ putative late blight resistance protein homologs. The transcript level of CA05g17730 differed between ‘PBC932’ and ‘77013’. The structure of the CA05g17730 gene also differed between ‘PBC932’ and ‘77013’. Conclusions We narrowed down the QTL interval to a region containing five genes. These results will be useful for further research on the mechanisms of resistance to anthracnose, and for marker assisted selection for anthracnose-resistant capsicum lines. |
topic |
Pepper Anthracnose Fine mapping Colletotrichum scovillei QTL |
url |
http://link.springer.com/article/10.1186/s12870-019-2115-1 |
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