Hypocholesterolemic Effect and In Vitro Pancreatic Lipase Inhibitory Activity of an Opuntia ficus-indica Extract
Cholesterol control is fundamental for prevention of cardiovascular disorders. In this work, the hypocholesterolemic activity of an aqueous Opuntia ficus-indica extract (AOE) was tested in triton-induced mice. The inhibitory activity on pancreatic lipase enzyme was evaluated in vitro by the same ext...
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doaj-0a78ec7f52524d86bd3e16edec1ad6702020-11-25T00:35:47ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/837452837452Hypocholesterolemic Effect and In Vitro Pancreatic Lipase Inhibitory Activity of an Opuntia ficus-indica ExtractEduardo Padilla-Camberos0Jose Miguel Flores-Fernandez1Ofelia Fernandez-Flores2Yanet Gutierrez-Mercado3Joel Carmona-de la Luz4Fabiola Sandoval-Salas5Carlos Mendez-Carreto6Kirk Allen7Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, MexicoBiotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, MexicoBiotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, MexicoBiotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, MexicoBiotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, AC, Av. Normalistas 800, Colonia Colinas de la Normal, 44270 Guadalajara, JAL, MexicoInstituto Tecnológico Superior de Perote, Km 2.5 Carretera Perote-México Colonia Centro, 91270 Perote, VER, MexicoInstituto Tecnológico Superior de Perote, Km 2.5 Carretera Perote-México Colonia Centro, 91270 Perote, VER, MexicoLancaster Medical School, Lancaster LA1 4YG, UKCholesterol control is fundamental for prevention of cardiovascular disorders. In this work, the hypocholesterolemic activity of an aqueous Opuntia ficus-indica extract (AOE) was tested in triton-induced mice. The inhibitory activity on pancreatic lipase enzyme was evaluated in vitro by the same extract. Furthermore, polyphenol content of the extract was evaluated. Hypercholesterolemia was induced in three groups of mice by intraperitoneal administration of Triton WR-1339. After induction of hypercholesterolemia, the groups were treated with an AOE (500 mg/kg) and saline solution and the positive control group with orlistat, respectively. Cholesterol levels were measured 24 h later in peripheral blood. The levels of blood cholesterol after administration of AOE significantly decreased compared to negative control. The inhibitory activity of AOE on pancreatic lipase enzyme was evaluated at concentrations from 60 to 1000 μg/mL. The AOE inhibited the pancreatic lipase with an IC50 = 588.5 μg/mL. The AOE had a high content of polyphenolic compounds. These results show that AOE is able to prevent hypercholesterolemia by pancreatic lipase inhibition, in part due to its polyphenolic compounds.http://dx.doi.org/10.1155/2015/837452 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eduardo Padilla-Camberos Jose Miguel Flores-Fernandez Ofelia Fernandez-Flores Yanet Gutierrez-Mercado Joel Carmona-de la Luz Fabiola Sandoval-Salas Carlos Mendez-Carreto Kirk Allen |
spellingShingle |
Eduardo Padilla-Camberos Jose Miguel Flores-Fernandez Ofelia Fernandez-Flores Yanet Gutierrez-Mercado Joel Carmona-de la Luz Fabiola Sandoval-Salas Carlos Mendez-Carreto Kirk Allen Hypocholesterolemic Effect and In Vitro Pancreatic Lipase Inhibitory Activity of an Opuntia ficus-indica Extract BioMed Research International |
author_facet |
Eduardo Padilla-Camberos Jose Miguel Flores-Fernandez Ofelia Fernandez-Flores Yanet Gutierrez-Mercado Joel Carmona-de la Luz Fabiola Sandoval-Salas Carlos Mendez-Carreto Kirk Allen |
author_sort |
Eduardo Padilla-Camberos |
title |
Hypocholesterolemic Effect and In Vitro Pancreatic Lipase Inhibitory Activity of an Opuntia ficus-indica Extract |
title_short |
Hypocholesterolemic Effect and In Vitro Pancreatic Lipase Inhibitory Activity of an Opuntia ficus-indica Extract |
title_full |
Hypocholesterolemic Effect and In Vitro Pancreatic Lipase Inhibitory Activity of an Opuntia ficus-indica Extract |
title_fullStr |
Hypocholesterolemic Effect and In Vitro Pancreatic Lipase Inhibitory Activity of an Opuntia ficus-indica Extract |
title_full_unstemmed |
Hypocholesterolemic Effect and In Vitro Pancreatic Lipase Inhibitory Activity of an Opuntia ficus-indica Extract |
title_sort |
hypocholesterolemic effect and in vitro pancreatic lipase inhibitory activity of an opuntia ficus-indica extract |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Cholesterol control is fundamental for prevention of cardiovascular disorders. In this work, the hypocholesterolemic activity of an aqueous Opuntia ficus-indica extract (AOE) was tested in triton-induced mice. The inhibitory activity on pancreatic lipase enzyme was evaluated in vitro by the same extract. Furthermore, polyphenol content of the extract was evaluated. Hypercholesterolemia was induced in three groups of mice by intraperitoneal administration of Triton WR-1339. After induction of hypercholesterolemia, the groups were treated with an AOE (500 mg/kg) and saline solution and the positive control group with orlistat, respectively. Cholesterol levels were measured 24 h later in peripheral blood. The levels of blood cholesterol after administration of AOE significantly decreased compared to negative control. The inhibitory activity of AOE on pancreatic lipase enzyme was evaluated at concentrations from 60 to 1000 μg/mL. The AOE inhibited the pancreatic lipase with an IC50 = 588.5 μg/mL. The AOE had a high content of polyphenolic compounds. These results show that AOE is able to prevent hypercholesterolemia by pancreatic lipase inhibition, in part due to its polyphenolic compounds. |
url |
http://dx.doi.org/10.1155/2015/837452 |
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