Detecting SARS-CoV-2 in the Breath of COVID-19 Patients
In the COVID-19 outbreak year 2020, a consensus was reached on the fact that SARS-CoV-2 spreads through aerosols. However, finding an efficient method to detect viruses in aerosols to monitor the risk of similar infections and enact effective control remains a great challenge. Our study aimed to bui...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2021-03-01
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Series: | Frontiers in Medicine |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2021.604392/full |
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doaj-0a94036fb325407189dfe27328e76c0d |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoguang Li Jing Li Qinggang Ge Yuguang Du Guoqiang Li Wei Li Tong Zhang Lei Tan Runqiang Zhang Xiaoning Yuan He Zhang Chen Zhang Wenjun Liu Wei Ding Liang Sun Ke Chen Zhuo Wang Ning Shen Jun Lu |
spellingShingle |
Xiaoguang Li Jing Li Qinggang Ge Yuguang Du Guoqiang Li Wei Li Tong Zhang Lei Tan Runqiang Zhang Xiaoning Yuan He Zhang Chen Zhang Wenjun Liu Wei Ding Liang Sun Ke Chen Zhuo Wang Ning Shen Jun Lu Detecting SARS-CoV-2 in the Breath of COVID-19 Patients Frontiers in Medicine SARS-CoV-2 COVID-19 exhaled breath swirling aerosol collector virus detection |
author_facet |
Xiaoguang Li Jing Li Qinggang Ge Yuguang Du Guoqiang Li Wei Li Tong Zhang Lei Tan Runqiang Zhang Xiaoning Yuan He Zhang Chen Zhang Wenjun Liu Wei Ding Liang Sun Ke Chen Zhuo Wang Ning Shen Jun Lu |
author_sort |
Xiaoguang Li |
title |
Detecting SARS-CoV-2 in the Breath of COVID-19 Patients |
title_short |
Detecting SARS-CoV-2 in the Breath of COVID-19 Patients |
title_full |
Detecting SARS-CoV-2 in the Breath of COVID-19 Patients |
title_fullStr |
Detecting SARS-CoV-2 in the Breath of COVID-19 Patients |
title_full_unstemmed |
Detecting SARS-CoV-2 in the Breath of COVID-19 Patients |
title_sort |
detecting sars-cov-2 in the breath of covid-19 patients |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Medicine |
issn |
2296-858X |
publishDate |
2021-03-01 |
description |
In the COVID-19 outbreak year 2020, a consensus was reached on the fact that SARS-CoV-2 spreads through aerosols. However, finding an efficient method to detect viruses in aerosols to monitor the risk of similar infections and enact effective control remains a great challenge. Our study aimed to build a swirling aerosol collection (SAC) device to collect viral particles in exhaled breath and subsequently detect SARS-CoV-2 using reverse transcription polymerase chain reaction (RT-PCR). Laboratory tests of the SAC device using aerosolized SARS-CoV-2 pseudovirus indicated that the SAC device can produce a positive result in only 10 s, with a collection distance to the source of 10 cm in a biosafety chamber, when the release rate of the pseudovirus source was 1,000,000 copies/h. Subsequent clinical trials of the device showed three positives and 14 negatives out of 27 patients in agreement with pharyngeal swabs, and 10 patients obtained opposite results, while no positive results were found in a healthy control group (n = 12). Based on standard curve calibration, several thousand viruses per minute were observed in the tested exhalations. Furthermore, referring to the average tidal volume data of adults, it was estimated that an exhaled SARS-CoV-2 concentration of approximately one copy/mL is detectable for COVID-19 patients. This study validates the original concept of breath detection of SARS-CoV-2 using SAC combined with RT-PCR. |
topic |
SARS-CoV-2 COVID-19 exhaled breath swirling aerosol collector virus detection |
url |
https://www.frontiersin.org/articles/10.3389/fmed.2021.604392/full |
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doaj-0a94036fb325407189dfe27328e76c0d2021-03-17T04:54:31ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2021-03-01810.3389/fmed.2021.604392604392Detecting SARS-CoV-2 in the Breath of COVID-19 PatientsXiaoguang Li0Jing Li1Qinggang Ge2Yuguang Du3Guoqiang Li4Wei Li5Tong Zhang6Lei Tan7Runqiang Zhang8Xiaoning Yuan9He Zhang10Chen Zhang11Wenjun Liu12Wei Ding13Liang Sun14Ke Chen15Zhuo Wang16Ning Shen17Jun Lu18Department of Infectious Diseases, Peking University Third Hospital, Beijing, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaDepartment of Infectious Diseases, Peking University Third Hospital, Beijing, ChinaState Key Laboratory of Biochemical Engineering, Institute of Process Engineering and Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing, ChinaCommune of Scientific Engineers, Institute of Physics, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaDepartment of Infectious Diseases, YouAn Hospital, Capital Medical University, Beijing, ChinaState Key Laboratory of Hydroscience and Engineering, Department of Energy and Power Engineering, Tsinghua University, Beijing, ChinaState Key Laboratory of Hydroscience and Engineering, Department of Energy and Power Engineering, Tsinghua University, Beijing, ChinaDepartment of Infectious Diseases, Peking University Third Hospital, Beijing, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Biochemical Engineering, Institute of Process Engineering and Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCommune of Scientific Engineers, Institute of Physics, Chinese Academy of Sciences, Beijing, ChinaCommune of Scientific Engineers, Institute of Physics, Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Biochemical Engineering, Institute of Process Engineering and Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing, ChinaDepartment of Infectious Diseases, Peking University Third Hospital, Beijing, ChinaCommune of Scientific Engineers, Institute of Physics, Chinese Academy of Sciences, Beijing, ChinaIn the COVID-19 outbreak year 2020, a consensus was reached on the fact that SARS-CoV-2 spreads through aerosols. However, finding an efficient method to detect viruses in aerosols to monitor the risk of similar infections and enact effective control remains a great challenge. Our study aimed to build a swirling aerosol collection (SAC) device to collect viral particles in exhaled breath and subsequently detect SARS-CoV-2 using reverse transcription polymerase chain reaction (RT-PCR). Laboratory tests of the SAC device using aerosolized SARS-CoV-2 pseudovirus indicated that the SAC device can produce a positive result in only 10 s, with a collection distance to the source of 10 cm in a biosafety chamber, when the release rate of the pseudovirus source was 1,000,000 copies/h. Subsequent clinical trials of the device showed three positives and 14 negatives out of 27 patients in agreement with pharyngeal swabs, and 10 patients obtained opposite results, while no positive results were found in a healthy control group (n = 12). Based on standard curve calibration, several thousand viruses per minute were observed in the tested exhalations. Furthermore, referring to the average tidal volume data of adults, it was estimated that an exhaled SARS-CoV-2 concentration of approximately one copy/mL is detectable for COVID-19 patients. This study validates the original concept of breath detection of SARS-CoV-2 using SAC combined with RT-PCR.https://www.frontiersin.org/articles/10.3389/fmed.2021.604392/fullSARS-CoV-2COVID-19exhaled breathswirling aerosol collectorvirus detection |