Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR

• Main Authors: Seung-Yeol Lee, Mi-Chi Yea, Chang-Gi Back, Kwang-Shik Choi, In-Kyu Kang, Su-Heon Lee, Hee-Young Jung
• Format: Article
• Language: English
• Published: Hanrimwon Publishing Company 2014-12-01
• Series: The Plant Pathology Journal
• Subjects: CNRMV; CGRMV; Duplex RT-PCR; Infection rate
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262299

The incidence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) have recently been occurred in Korea, posing a problem for sweet cherry cultivation. Since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. In this study, the incidence of CNRMV and CGRMV in sweet cherry in Gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (RT-PCR) method that can detect both viruses in a single reaction. CNRMV and CGRMV co-infection rates were 29.6%, 53.6%, and 17.6%, respectively, in samples collected from three different sites (Daegu, Gyeongju and Gyeongsan) in Gyeongbuk province during 2012 and 2013. This duplex RT-PCR method offers a simple, rapid, and effective way of identifying CNRMV and CGRMV simultaneously in sweet cherry trees, which can aid in the management of viral infections that could undermine yield.