A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples.

A method for manual and automated multiplex RNAscope in situ hybridization and immunocytochemistry on cytospin samples.

In situ analysis of biomarkers is essential for clinical diagnosis and research purposes. The increasing need to understand the molecular signature of pathologies has led to the blooming of ultrasensitive and multiplexable techniques that combine in situ hybridization (ISH) and immunohistochemistry...

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Bibliographic Details
Main Authors: Sara Chan, Audrey Filézac de L'Etang, Linda Rangell, Patrick Caplazi, John B Lowe, Valentina Romeo
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC6245747?pdf=render

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