Three-Dimensional Mapping of Retrograde Multi-Labeled Motor Neuron Columns in the Spinal Cord
The quantification and distribution characteristics of spinal motor neurons play important roles in the study of spinal cord and peripheral nerve injury and repair. In most research, the sole retrograde labeling of each nerve or muscle could not simultaneously obtain the distributions of different m...
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doaj-0aa279ad53e14bc78b726e386b345f0f2021-04-28T23:00:21ZengMDPI AGPhotonics2304-67322021-04-01814514510.3390/photonics8050145Three-Dimensional Mapping of Retrograde Multi-Labeled Motor Neuron Columns in the Spinal CordJianyi Xu0Xiaofeng Yin1Yisong Qi2Bo Chen3Yusha Li4Peng Wan5Yingtao Yao6Dan Zhu7Baoguo Jiang8Tingting Yu9Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Trauma and Orthopaedics, Peking University People’s Hospital, Beijing 100044, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Trauma and Orthopaedics, Peking University People’s Hospital, Beijing 100044, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Trauma and Orthopaedics, Peking University People’s Hospital, Beijing 100044, ChinaBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, ChinaThe quantification and distribution characteristics of spinal motor neurons play important roles in the study of spinal cord and peripheral nerve injury and repair. In most research, the sole retrograde labeling of each nerve or muscle could not simultaneously obtain the distributions of different motor neuron subpopulations. Therefore, it did not allow mapping of spatial relationships of different motor neuron columns for disclosing the functional relationship of different nerve branches. Here, we combined the multiple retrograde labeling, optical clearing, and imaging for three-dimensional (3D) visualization of motor neurons of multiple brachial plexus branches. After screening fluorescent tracers by the labeling feasibility of motor neurons and fluorescence compatibility with optical clearing, we performed mapping and quantification of the motor neurons of ulnar, median, and radial nerves in the spinal cord, then disclosed the relative spatial distribution among different neuronal subpopulations. This work will provide valuable mapping data for the understanding of the functional relationships among brachial plexus branches, hopefully facilitating the study of regeneration of axons and remodeling of motor neurons in peripheral nerve repair.https://www.mdpi.com/2304-6732/8/5/145motor neuronspinal cordperipheral nerveoptical clearing3D imagingretrograde tracing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jianyi Xu Xiaofeng Yin Yisong Qi Bo Chen Yusha Li Peng Wan Yingtao Yao Dan Zhu Baoguo Jiang Tingting Yu |
spellingShingle |
Jianyi Xu Xiaofeng Yin Yisong Qi Bo Chen Yusha Li Peng Wan Yingtao Yao Dan Zhu Baoguo Jiang Tingting Yu Three-Dimensional Mapping of Retrograde Multi-Labeled Motor Neuron Columns in the Spinal Cord Photonics motor neuron spinal cord peripheral nerve optical clearing 3D imaging retrograde tracing |
author_facet |
Jianyi Xu Xiaofeng Yin Yisong Qi Bo Chen Yusha Li Peng Wan Yingtao Yao Dan Zhu Baoguo Jiang Tingting Yu |
author_sort |
Jianyi Xu |
title |
Three-Dimensional Mapping of Retrograde Multi-Labeled Motor Neuron Columns in the Spinal Cord |
title_short |
Three-Dimensional Mapping of Retrograde Multi-Labeled Motor Neuron Columns in the Spinal Cord |
title_full |
Three-Dimensional Mapping of Retrograde Multi-Labeled Motor Neuron Columns in the Spinal Cord |
title_fullStr |
Three-Dimensional Mapping of Retrograde Multi-Labeled Motor Neuron Columns in the Spinal Cord |
title_full_unstemmed |
Three-Dimensional Mapping of Retrograde Multi-Labeled Motor Neuron Columns in the Spinal Cord |
title_sort |
three-dimensional mapping of retrograde multi-labeled motor neuron columns in the spinal cord |
publisher |
MDPI AG |
series |
Photonics |
issn |
2304-6732 |
publishDate |
2021-04-01 |
description |
The quantification and distribution characteristics of spinal motor neurons play important roles in the study of spinal cord and peripheral nerve injury and repair. In most research, the sole retrograde labeling of each nerve or muscle could not simultaneously obtain the distributions of different motor neuron subpopulations. Therefore, it did not allow mapping of spatial relationships of different motor neuron columns for disclosing the functional relationship of different nerve branches. Here, we combined the multiple retrograde labeling, optical clearing, and imaging for three-dimensional (3D) visualization of motor neurons of multiple brachial plexus branches. After screening fluorescent tracers by the labeling feasibility of motor neurons and fluorescence compatibility with optical clearing, we performed mapping and quantification of the motor neurons of ulnar, median, and radial nerves in the spinal cord, then disclosed the relative spatial distribution among different neuronal subpopulations. This work will provide valuable mapping data for the understanding of the functional relationships among brachial plexus branches, hopefully facilitating the study of regeneration of axons and remodeling of motor neurons in peripheral nerve repair. |
topic |
motor neuron spinal cord peripheral nerve optical clearing 3D imaging retrograde tracing |
url |
https://www.mdpi.com/2304-6732/8/5/145 |
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