16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters
Photoperiod is an important factor in stimulating broiler performance in commercial poultry practice. However, the mechanism by which photoperiod affects the performance of broiler chickens has not been adequately explored. The current study evaluated the effects of three different photoperiod regim...
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doaj-0ab5b33db6224ce98b266d86b2e3f0b82020-11-24T22:01:19ZengPeerJ Inc.PeerJ2167-83592018-02-016e439010.7717/peerj.439016S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roostersJun Wang0Lucky T. Nesengani1Yongsheng Gong2Yujiang Yang3Wenfa Lu4College of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaPhotoperiod is an important factor in stimulating broiler performance in commercial poultry practice. However, the mechanism by which photoperiod affects the performance of broiler chickens has not been adequately explored. The current study evaluated the effects of three different photoperiod regimes (short day (LD) = 8 h light, control (CTR) = 12.5 h light, and long day (SD) = 16 h light) on the cecal microbiota of broiler roosters by sequencing bacterial 16S rRNA genes. At the phylum level, the dominant bacteria were Firmicutes (CTR: 68%, SD: 69%, LD: 67%) and Bacteroidetes (CTR: 25%, SD: 26%, and LD: 28%). There was a greater abundance of Proteobacteria (p < 0.01) and Cyanobacteria (p < 0.05) in chickens in the LD group than in those in the CTR group. A significantly greater abundance of Actinobacteria was observed in CTR chickens than in SD and LD chickens (p < 0.01). The abundance of Deferribacteres was significantly higher in LD chickens than in SD chickens (p < 0.01). Fusobacteria and Proteobacteria were more abundant in SD chickens than in CTR and LD chickens. The predicted functional properties indicate that cellular processes may be influenced by photoperiod. Conversely, carbohydrate metabolism was enhanced in CTR chickens as compared to that in SD and LD chickens. The current results indicate that different photoperiod regimes may influence the abundance of specific bacterial populations and then contribute to differences in the functional properties of gut microbiota of broiler roosters.https://peerj.com/articles/4390.pdfPhotoperiodRoosterCecal microbiota16S rRNA gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun Wang Lucky T. Nesengani Yongsheng Gong Yujiang Yang Wenfa Lu |
spellingShingle |
Jun Wang Lucky T. Nesengani Yongsheng Gong Yujiang Yang Wenfa Lu 16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters PeerJ Photoperiod Rooster Cecal microbiota 16S rRNA gene |
author_facet |
Jun Wang Lucky T. Nesengani Yongsheng Gong Yujiang Yang Wenfa Lu |
author_sort |
Jun Wang |
title |
16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters |
title_short |
16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters |
title_full |
16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters |
title_fullStr |
16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters |
title_full_unstemmed |
16S rRNA gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters |
title_sort |
16s rrna gene sequencing reveals effects of photoperiod on cecal microbiota of broiler roosters |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2018-02-01 |
description |
Photoperiod is an important factor in stimulating broiler performance in commercial poultry practice. However, the mechanism by which photoperiod affects the performance of broiler chickens has not been adequately explored. The current study evaluated the effects of three different photoperiod regimes (short day (LD) = 8 h light, control (CTR) = 12.5 h light, and long day (SD) = 16 h light) on the cecal microbiota of broiler roosters by sequencing bacterial 16S rRNA genes. At the phylum level, the dominant bacteria were Firmicutes (CTR: 68%, SD: 69%, LD: 67%) and Bacteroidetes (CTR: 25%, SD: 26%, and LD: 28%). There was a greater abundance of Proteobacteria (p < 0.01) and Cyanobacteria (p < 0.05) in chickens in the LD group than in those in the CTR group. A significantly greater abundance of Actinobacteria was observed in CTR chickens than in SD and LD chickens (p < 0.01). The abundance of Deferribacteres was significantly higher in LD chickens than in SD chickens (p < 0.01). Fusobacteria and Proteobacteria were more abundant in SD chickens than in CTR and LD chickens. The predicted functional properties indicate that cellular processes may be influenced by photoperiod. Conversely, carbohydrate metabolism was enhanced in CTR chickens as compared to that in SD and LD chickens. The current results indicate that different photoperiod regimes may influence the abundance of specific bacterial populations and then contribute to differences in the functional properties of gut microbiota of broiler roosters. |
topic |
Photoperiod Rooster Cecal microbiota 16S rRNA gene |
url |
https://peerj.com/articles/4390.pdf |
work_keys_str_mv |
AT junwang 16srrnagenesequencingrevealseffectsofphotoperiodoncecalmicrobiotaofbroilerroosters AT luckytnesengani 16srrnagenesequencingrevealseffectsofphotoperiodoncecalmicrobiotaofbroilerroosters AT yongshenggong 16srrnagenesequencingrevealseffectsofphotoperiodoncecalmicrobiotaofbroilerroosters AT yujiangyang 16srrnagenesequencingrevealseffectsofphotoperiodoncecalmicrobiotaofbroilerroosters AT wenfalu 16srrnagenesequencingrevealseffectsofphotoperiodoncecalmicrobiotaofbroilerroosters |
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1725840301985103872 |