Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies.
The in situ proximity ligation assay (isPLA) is an increasingly used technology for in situ detection of protein interactions, post-translational modifications, and spatial relationships of antigens in cells and tissues, in general. In order to test its performance we compared isPLA with immunofluor...
Main Authors: | Bernhard Zatloukal, Iris Kufferath, Andrea Thueringer, Ulf Landegren, Kurt Zatloukal, Johannes Haybaeck |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4010503?pdf=render |
Similar Items
-
p62/Sequestosome-1 Is Indispensable for Maturation and Stabilization of Mallory-Denk Bodies.
by: Pooja Lahiri, et al.
Published: (2016-01-01) -
The PPARα Agonist Fenofibrate Prevents Formation of Protein Aggregates (Mallory-Denk bodies) in a Murine Model of Steatohepatitis-like Hepatotoxicity
by: Aniket Nikam, et al.
Published: (2018-08-01) -
Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes
by: Axel Klaesson, et al.
Published: (2018-03-01) -
Improving precision of proximity ligation assay by amplified single molecule detection.
by: Rongqin Ke, et al.
Published: (2013-01-01) -
Sensitive detection of Aβ protofibrils by proximity ligation - relevance for Alzheimer's disease
by: Gustafsdottir Sigrun, et al.
Published: (2010-10-01)