Morphometric Analysis of Larval Rostellar Hooks in Taenia multiceps of Sheep in Iran and Its Association with Mitochondrial Gene Variability

• Main Authors: Sima Rostami, Robin Nicholas Beech, Reza Salavati, Mohammad Reza Baneshi, Hossein Kamyabi, Majid Fasihi Harandi
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2013-12-01
• Series: Iranian Journal of Parasitology
• Subjects: Hook morphometry; Iran; Mitochondrial gene; Sheep; Taenia multiceps
• Online Access: https://ijpa.tums.ac.ir/index.php/ijpa/article/view/452
• ISSN: 1735-7020; 2008-238X

  Background: The purposes of the present study were morphometric characteri-zation of rostellar hooks of Taenia multiceps and to investigate the association of hook length variation and the variability within two mitochondrial genes of sheep isolates of the parasite. Methods: Up to 4500 sheep brains were examined for the presence of C. cerebralis. Biometric characters based on the larval rostellar hook size were measured for each individual isolate. Representative mitochondrial CO1 and 12S rRNA gene se-quences for each of the isolates were obtained from NCBI GenBank. Morphomet-ric and genetic data were analyzed using cluster analysis, Interclass Correlation Co-efficient (ICC) and random effects model. Results: One hundred and fourteen sheep (2.5%) were found infected with the coenuri. The minimum and maximum number of scoleces per cyst was 40 and 550 respectively. Each scolex contained 22–27 hooks arranged in two rows of large and small hooks. The average total length of the large and small hooks was 158.9 and 112.1 μm, respectively. Using ICC, statistically significant clusters of different hook sizes were identified within the isolates. The length of the large and small hooks was significantly associated with the variability in mitochondrial 12S rRNA gene. Conclusion: Taenia multiceps, is a relatively important zoonotic infection in Iranian sheep with the prevalence rate of 2.5%. Hook length analysis revealed statistically significant difference among individual isolates. Associations between the rostellar hook length and variability in the mitochondrial 12S rRNA was documented.