A Compact Immunoassay Platform Based on a Multicapillary Glass Plate

• Main Authors: Shuhua Xue, Hulie Zeng, Jianmin Yang, Hizuru Nakajima, Katsumi Uchiyama
• Format: Article
• Language: English
• Published: MDPI AG 2014-05-01
• Series: Sensors
• Subjects: multicapillary glass plate; compact immunoassay; enzyme-linked immunosorbent assay
• Online Access: http://www.mdpi.com/1424-8220/14/5/9132

A highly sensitive, rapid immunoassay performed in the multi-channels of a micro-well array consisting of a multicapillary glass plate (MCP) and a polydimethylsiloxane (PDMS) slide is described. The micro-dimensions and large surface area of the MCP permitted the diffusion distance to be decreased and the reaction efficiency to be increased. To confirm the concept of the method, human immunoglobulin A (h-IgA) was measured using both the proposed immunoassay system and the traditional 96-well plate method. The proposed method resulted in a 1/5-fold decrease of immunoassay time, and a 1/56-fold cut in reagent consumption with a 0.05 ng/mL of limit of detection (LOD) for IgA. The method was also applied to saliva samples obtained from healthy volunteers. The results correlated well to those obtained by the 96-well plate method. The method has the potential for use in disease diagnostic or on-site immunoassays.